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Antibacterial Activity of Silver-nanoparticles Against Staphylococcus aureus and Escherichia coli  

Kim, Soo-Hwan (Department of Smart Foods and Drugs, Inje University)
Lee, Hyeong-Seon (Department of Smart Foods and Drugs, Inje University)
Ryu, Deok-Seon (Department of Smart Foods and Drugs, Inje University)
Choi, Soo-Jae (Department of Smart Foods and Drugs, Inje University)
Lee, Dong-Seok (Department of Smart Foods and Drugs, Inje University)
Publication Information
Microbiology and Biotechnology Letters / v.39, no.1, 2011 , pp. 77-85 More about this Journal
Abstract
The antibacterial activities of silver nanoparticles (Ag-NPs) were studied with respect to Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli by observing the bacterial cells treated or not with Ag-NPs by FE-SEM as well as measuring the growth curves, formation of bactericidal ROS, protein leakage, and lactate dehydrogenase activity involved in the respiratory chain. Bacterial cells were treated with Ag-NPs powder, and the growth rates were investigated under varying concentrations of Ag-NPs, incubation times, incubation temperatures, and pHs. As a result, S. aureus and E. coli were shown to be substantially inhibited by Ag-NPs, and the antibacterial activity of Ag-NPs did not fluctuate with temperature or pH. These results suggest that Ag-NPs could be used as an effective antibacterial material.
Keywords
Staphylococcus aureus; Escherichia coli; silver nanoparticles (Ag-NPs); antibacterial activity;
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