Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of Methane Oxidation by a Methanotroph Isolated from a Landfill Cover Soil, South Korea

  • Lee, Eun-Hee (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Yi, Tae-Woo (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Moon, Kyung-Eun (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Park, Hyun-Jung (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Ryu, Hee-Wook (Department of Chemical Engineering, Soongsil University) ;
  • Cho, Kyung-Suk (Department of Environmental Science and Engineering, Ewha Womans University)
  • Received : 2011.02.01
  • Accepted : 2011.04.26
  • Published : 2011.07.28
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Abstract

A methane-oxidizing bacterium was isolated from the enriched culture of a landfill cover soil. The closest relative of the isolate, designated M6, is Methylocystis sp. Based on a kinetic analysis, the maximum specific methane oxidation rate and saturation constant were 4.93 mmol gdry cell $weight^{-1}{\cdot}h^{-1}$ and 23${\mu}M$, respectively. This was the first time a kinetic analysis was performed using pure methanotrophic culture. The methane oxidation by M6 was investigated in the presence of aromatic (m- and pxylene and ethylbenzene) or sulfur (hydrogen sulfide, dimethyl sulfide, methanthiol) compounds. The methane oxidation was inhibited by the presence of aromatic or sulfur compounds.

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References

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