Journal of Microbiology and Biotechnology

  • 제21권8호
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  • Pages.796-801
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  • 2011
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Characterization of Novel Plasmid p1B146 from Corynebacterium tuberculostearicum

  • Wieteska, Lukasz (Department of Medical Biochemistry, Medical University of Lodz) ;
  • Szewczyk, Eligia M. (Department of Pharmaceutical Microbiology, Medical University of Lodz) ;
  • Szemraj, Janusz (Department of Medical Biochemistry, Medical University of Lodz)
  • 투고 : 2011.10.10
  • 심사 : 2011.05.15
  • 발행 : 2011.08.28
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초록

Corynebacterium tuberculostearicum B146, a strain derived from healthy human skin, contains a medium copy plasmid, p1B146. This plasmid was cloned and its complete nucleotide sequence determined. As a result, p1B146 was found to be 4,2 kb in size with a 53% G+C content, plus six open reading frames (ORFs) were distinguished. According to a computer-assisted alignment, two of the ORFs exhibited significant similarities to already-known common plasmid proteins, the first being the RepA gene, responsible for plasmid replication via a rolling-circle mechanism, and the second being an FtsK-like protein, the function of which remains unclear. The presence and quantity of RNA fragments in the putative ORFs were also evaluated.

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