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http://dx.doi.org/10.4014/jmb.1101.01011

Characterization of Novel Plasmid p1B146 from Corynebacterium tuberculostearicum  

Wieteska, Lukasz (Department of Medical Biochemistry, Medical University of Lodz)
Szewczyk, Eligia M. (Department of Pharmaceutical Microbiology, Medical University of Lodz)
Szemraj, Janusz (Department of Medical Biochemistry, Medical University of Lodz)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.8, 2011 , pp. 796-801 More about this Journal
Abstract
Corynebacterium tuberculostearicum B146, a strain derived from healthy human skin, contains a medium copy plasmid, p1B146. This plasmid was cloned and its complete nucleotide sequence determined. As a result, p1B146 was found to be 4,2 kb in size with a 53% G+C content, plus six open reading frames (ORFs) were distinguished. According to a computer-assisted alignment, two of the ORFs exhibited significant similarities to already-known common plasmid proteins, the first being the RepA gene, responsible for plasmid replication via a rolling-circle mechanism, and the second being an FtsK-like protein, the function of which remains unclear. The presence and quantity of RNA fragments in the putative ORFs were also evaluated.
Keywords
Corynebacterium; plasmid; rolling-circle mechanism; FtsK-like protein;
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