Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Characterization of Novel Plasmid p1B146 from Corynebacterium tuberculostearicum

  • Wieteska, Lukasz (Department of Medical Biochemistry, Medical University of Lodz) ;
  • Szewczyk, Eligia M. (Department of Pharmaceutical Microbiology, Medical University of Lodz) ;
  • Szemraj, Janusz (Department of Medical Biochemistry, Medical University of Lodz)
  • Received : 2011.10.10
  • Accepted : 2011.05.15
  • Published : 2011.08.28
Download PDF
⟨ Previous Next ⟩

Abstract

Corynebacterium tuberculostearicum B146, a strain derived from healthy human skin, contains a medium copy plasmid, p1B146. This plasmid was cloned and its complete nucleotide sequence determined. As a result, p1B146 was found to be 4,2 kb in size with a 53% G+C content, plus six open reading frames (ORFs) were distinguished. According to a computer-assisted alignment, two of the ORFs exhibited significant similarities to already-known common plasmid proteins, the first being the RepA gene, responsible for plasmid replication via a rolling-circle mechanism, and the second being an FtsK-like protein, the function of which remains unclear. The presence and quantity of RNA fragments in the putative ORFs were also evaluated.

Keywords

References

  1. Besemer, J. and M. Borodovsky. 1999. Heuristic approach to deriving models for gene finding. Nucleic Acids Res. 27: 3911- 3920. https://doi.org/10.1093/nar/27.19.3911
  2. Birnboim, H. C. and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 24: 1513-1523.
  3. Carapuca, E., A. R. Azzoni, D. M. F. Prazeres, G. A. Monteiro, and F. J. M. Mergulhao. 2007. Time-course determination of plasmid content in eukaryotic and prokaryotic cells using realtime PCR. Mol. Biotechnol. 37: 120-126. https://doi.org/10.1007/s12033-007-0007-3
  4. Deb, J. K. and N. Nat. 1999. Plasmids of corynebacteria. FEMS Microbiol. Lett. 175: 11-20. https://doi.org/10.1111/j.1574-6968.1999.tb13596.x
  5. Donachie, W. D. 2002. FtsK: Maxwell's Demon? Mol. Cell 9: 206-207. https://doi.org/10.1016/S1097-2765(02)00457-4
  6. Feurer, C., D. Clermont, F. Bimet, A. Candrea, M. Jackson, P. Glaser, C. Bizet, and C. Dauga. 2004. Taxonomic characterization of nine strains isolated from clinical and environmental specimens, and proposal of Corynebacterium tuberculostearicum sp. nov. Int. J. Syst. Evol. Microbiol. 54: 1055-1061. https://doi.org/10.1099/ijs.0.02907-0
  7. Gi-Seong, M., U. Wegmann, P. Gunning, M. J. Gasson, and A. Narbad. 2009. Isolation and characterization of a theta-type cryptic plasmid from Bifidobacterium longum FI10564. J. Microbiol. Biotechnol. 19: 403-408. https://doi.org/10.4014/jmb.0806.378
  8. Grohmann, E., G. Muth, and M. Espinosa. 2003. Conjugative plasmid transfer in Gram-positive bacteria. Microbiol. Mol. Biol. Rev. 67: 277-301. https://doi.org/10.1128/MMBR.67.2.277-301.2003
  9. Hottat, F., M. Coene, and C. Cocito. 1988. DNA methylation in leprosy-associated bacteria: Mycobacterium leprae and Corynebacterium tuberculostearicum. Med. Microbiol. Immunol. 177: 33-45.
  10. Kaneko, H., T. Tanaka, and K. Sakaguchi. 1979. Isolation and characterisation of a plasmid from Brevibacterium lactofermentum. Agric. Biol. Chem. 43: 867-868. https://doi.org/10.1271/bbb1961.43.867
  11. Khan, S. A. 1997. Rolling-circle replication of bacterial plasmids. Microbiol. Mol. Biol. Rev. 61: 442-455.
  12. Kono, M., M. Sasatsu, and T. Aoki. 1983. R plasmid in Corynebacterium xerosis strains. Antimicrob. Agents Chemother. 23: 506-508. https://doi.org/10.1128/AAC.23.3.506
  13. Mignard, S. and J. P. Flandrois. 2006. 16S rRNA sequencing in routine bacterial identification: A 30-month experiment. J. Microbiol. Methods 67: 574-581. https://doi.org/10.1016/j.mimet.2006.05.009
  14. Mikucka, A. 2003. Significance of Corynebacterium spp. in hospital infections. Post. Mikrobiol. 42: 403-418.
  15. Nath, N. and J. K. Deb. 1995. Partial characterization of small plasmids from Corynebacterium renale. Plasmid 34: 229-233. https://doi.org/10.1006/plas.1995.0009
  16. O'Riordan, K. and G. F. Fitzgerald. 1999. Molecular characterisation of a 5.75-kb cryptic plasmid from Bifidobacterium breve NCFB 2258 and determination of mode of replication. FEMS Microbiol. Lett. 174: 285-294. https://doi.org/10.1111/j.1574-6968.1999.tb13581.x
  17. Park, M. S., D. W. Shin, K. H. Lee, and G. E. Ji. 1999. Sequence analysis of plasmid pKJ50 from Bifidobacterium longum. Microbiology 145: 585-592. https://doi.org/10.1099/13500872-145-3-585
  18. Paviour, S., S. Musaad, S. Roberts, G. Taylor, S. Taylor, K. Shore, S. Lang, and D. Holland. 2003. Corynebacterium species isolated from patients with mastitis. Clin. Infect. Dis. 36: 1434- 1440.
  19. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
  20. Sangrador-Vegas, A., C. Stanton, and D. van Sinderen. 2007. Characterization of plasmid pASV479 from Bifidobacterium pseudolongum subsp. globosum and its use for expression vector construction. Plasmid 58: 140-147. https://doi.org/10.1016/j.plasmid.2007.02.004
  21. Soding, J. 2005. Protein homology detection by HMM-HMM comparison. Bioinformatics 21: 951-960. https://doi.org/10.1093/bioinformatics/bti125
  22. Tauch, A., O. Kaiser, T. Hain, A. Goesmann, B. Weisshaar, A. Albersmeier, et al. 2005. Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora. J. Bacteriol. 187: 4671-4682. https://doi.org/10.1128/JB.187.13.4671-4682.2005
  23. Tsuchida, Y., S. Kimura, N. Suzuki, M. Inui, and H. Yukawa. 2009. Characterization of a new 2.4-kb plazmid of Corynebacterium casei and development of stable corynebacterial cloning vector. Appl. Microbiol. Biotechnol. 81: 1107-1115. https://doi.org/10.1007/s00253-008-1746-1
  24. Venkova-Canova, T., M. Patek, and J. Nesvera. 2004. Characterization of the cryptic plasmid pCC1 from Corynebacterium callunae and its use for vector construction. Plasmid 51: 54-60. https://doi.org/10.1016/j.plasmid.2003.09.002
  25. Wang, Z. and G. Wang. 2004. APD: The antimicrobial peptide database. Nucleic Acids Res. 32: D590-D592. https://doi.org/10.1093/nar/gkh025