Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effect of Methyl-Beta-Cyclodextrin (MBCD) on Cryopreservation of Bull Spermatozoa

소 정액의 동결 보존시 Methyl-Beta-Cyclodextrin (MBCD)의 영향

  • Lee, Kyung-Jin (College of Animal Life Sciences, Kangwon National University) ;
  • Seo, Gi-Beom (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Yong-Seung (College of Animal Life Sciences, Kangwon National University) ;
  • Yoo, Han-Jun (College of Animal Life Sciences, Kangwon National University) ;
  • Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University) ;
  • Lee, Seung-Hwan (National Institute of Animal Science) ;
  • Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
  • 이경진 (강원대학교 동물생명과학대학) ;
  • 서기범 (강원대학교 동물생명과학대학) ;
  • 이용승 (강원대학교 동물생명과학대학) ;
  • 유한준 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의과대학) ;
  • 이승환 (국립축산과학원) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 박춘근 (강원대학교 동물생명과학대학)
  • Received : 2011.08.29
  • Accepted : 2011.09.05
  • Published : 2011.09.30
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Abstract

The objective of this study was to evaluate efficiency of methyl-beta-cyclodextrin (MBCD) in the sperm preservation of bull. For this study, the freezing of diluted semen were added with Triladyl containing 20% egg-yolk and/or 0, 1, 5, 10 and 20 mM MBCD before freezing process. Analysis of viability in frozen-thawed sperm was estimated by SYBR14/PI double stain, hypoosmotic swelling test(HOST) and acrosome damage with FITC-PNA, and mitochondria activation with Rhodamin123 by flow-cytometry. The sperm viability was significantly in 0 mM and 5 mM concentrations of MBCD than other groups (p<0.05). However, the HOST was significantly lower at 20 mM concentration of MBCD than other concentrations (p<0.05). In addition, acrosome damage and mitochondria activation rates were significantly lower at 20 mM concentration of MBCD than other groups (p<0.05). In conclusion, the viability of sperm was not significantly different among concentrations of MBCD 0, 5 and 10 mM, but MBCD 20 mM was significantly lower than other groups. In addition, as concentrations of MBCD was high, HOST, acrosome damage and mitochondria activation rates had a negative effect in bull sperm.

Keywords

References

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