Browse > Article

Effect of Methyl-Beta-Cyclodextrin (MBCD) on Cryopreservation of Bull Spermatozoa  

Lee, Kyung-Jin (College of Animal Life Sciences, Kangwon National University)
Seo, Gi-Beom (College of Animal Life Sciences, Kangwon National University)
Lee, Yong-Seung (College of Animal Life Sciences, Kangwon National University)
Yoo, Han-Jun (College of Animal Life Sciences, Kangwon National University)
Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University)
Lee, Seung-Hwan (National Institute of Animal Science)
Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University)
Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
Publication Information
Reproductive and Developmental Biology / v.35, no.3, 2011 , pp. 295-300 More about this Journal
Abstract
The objective of this study was to evaluate efficiency of methyl-beta-cyclodextrin (MBCD) in the sperm preservation of bull. For this study, the freezing of diluted semen were added with Triladyl containing 20% egg-yolk and/or 0, 1, 5, 10 and 20 mM MBCD before freezing process. Analysis of viability in frozen-thawed sperm was estimated by SYBR14/PI double stain, hypoosmotic swelling test(HOST) and acrosome damage with FITC-PNA, and mitochondria activation with Rhodamin123 by flow-cytometry. The sperm viability was significantly in 0 mM and 5 mM concentrations of MBCD than other groups (p<0.05). However, the HOST was significantly lower at 20 mM concentration of MBCD than other concentrations (p<0.05). In addition, acrosome damage and mitochondria activation rates were significantly lower at 20 mM concentration of MBCD than other groups (p<0.05). In conclusion, the viability of sperm was not significantly different among concentrations of MBCD 0, 5 and 10 mM, but MBCD 20 mM was significantly lower than other groups. In addition, as concentrations of MBCD was high, HOST, acrosome damage and mitochondria activation rates had a negative effect in bull sperm.
Keywords
Methyl-beta-cyclodextrin; Cholesterol; Cryopreservation; Flow-cytometry; Bull sperm viability;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Amirat L, Tainturier D, Jeanneau L, Thorin C, Gerard O, Courtens JL, Anton M (2004): Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with optidyl, a commercial egg yolk extender. Theriogenology 61:895-907.   DOI   ScienceOn
2 Pike LJ, Miller JM (1998): Cholesterol depletion delocalizes phosphatidylinositol bisphosphate and inhibits hormone-stimulated phosphatidylinositol turnover. J Biol Chem 273:22298-22304.   DOI
3 Hartel S, Diehl HA, Ojeda SF (1998): Methyl-betacyclodextrins and liposomes as water-soluble carriers for cholesterol incorporation into membranes and its evaluation by a microenzymatic fluorescence assay and membrane fluidity-sensitive dyes. Anal Biochem 258:277-284.   DOI   ScienceOn
4 McGrath JJ (1988): Membrane transport properties. In: McGrath JJ, Diller KR, eds. Low Temperature Biotechnology: Emerging Applications and Engineering Contribution. New York, NY: ASME Press, BEDVol 10, HTD-Vol 98, 273-330.
5 Christian AE, Haynes MP, Phillips MC, Rothblat GH (1997): Use of cyclodextrins for manipulating cellular cholesterol content. J Lipid Res 38:2264-2272.
6 Visconti PE, Galantino-Homer H, Ning X, Moore GD, Valenzuela JP, Jorgue CJ, Alvarez JG, Kopf GS (1999): Colesterol efflux-mediated signal transduction in mammalian sperm beta-cyclodextrins initiate transmembrans signaling leading to an increase in protein tyrosine phosphorylation and capacitation. J Biol Chem 274:3235-42.   DOI
7 Jeong YJ, Kim MK, Song HJ, Kang EJ, Ock SA, Mohana Kumar B, Balasubramanian S, Rho GJ (2009): Effect of $\alpha$-tocopherol supplementation during boar semen cryopreservation on sperm characteristics and expression of apoptosis related genes. Cryobiology 58:181-189.   DOI   ScienceOn
8 Parks JE, Lynch DV (1992): Lipid composition and thermotropic phase behaviour of boar, bull, stallion and rooster sperm membranes. Cryobiology 29:255- 266.   DOI   ScienceOn
9 White IG (1993): Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reprod Fertil Dev 5 639-658.   DOI   ScienceOn
10 Yeagle PL (1985): Cholesterol and the cell membrane. Biochim Biophys Acta 822:267-287.   DOI   ScienceOn
11 Combes GB, Varner DD, Schroeder F, Burghardt RC, Blanchard TL (2000): Effect of cholesterol on the motility and plasma membrane integrity of frozen equine spermatozoa after thawing. J Reprod Fertil Suppl 56:127-132.
12 Holt, WV (2000): Fundamental aspects of sperm cryobiology: The importance of species and individual differences. Theriogenology 53:47-58.   DOI   ScienceOn
13 Thundathil J, Gil J, Januskauskas A, Larsson B, Soderquist L, Mapletoft R, Rodriguez-Martinez H(1999): Relationship between the proportion of capacitated spermatozoa present in frozen-thawed bull semen and fertility with artificial insemination. Int J Androl 22:366-373.   DOI   ScienceOn
14 Hammerstedt RH, Graham JK, Nolan JP (1990): Cryopreservation of mammalian sperm: What we ask them to survive. J Androl 11:73-88.
15 Curry MR (2000): Cryopreservation of semen from domestic livestock. Rev Reprod 5:46-52.   DOI   ScienceOn
16 Watson PF (1981): The effects of cold shock on sperm cell membranes. Pages 189-218 in Effects of Low Temperatures on Biological Membranes. G. J. Morris and A. Clarke, ed. Academic Press, London, UK.
17 Purdy PH, Graham JK (2004): Effect of adding cholesterol to bull sperm membranes on sperm capacitation, the acrosome reaction, and fertility. Biol Reprod 71:522-527.   DOI   ScienceOn
18 Visconti PE, Galantino-Homer H, Ning X, Moore GD, Valenzuela JP, Jorgez CJ, Alvarez JG, Kopf GS (1999): Cholesterol efflux-mediated signal transduction in mammalian sperm. beta-cyclodextrins initiate transmembrane signaling leading to an increase in protein tyrosine phosphorylation and capacitation. J Biol Chem 274:3235-3242.   DOI
19 Navratil AM, Bliss SP, Berghorn KA, Haughian JM, Farmerie TA, Graham JK, Clay CM, Roberson MS (2003): Constitutive localization of the gonadotropinreleasing hormone (GnRH) receptor to low density membrane microdomains is necessary for GnRH signaling to ERK. J Biol Chem 278:31593-31602.   DOI
20 Mercado E, De, Hernandez M, Sanz E, Rodriguez A, Gomez E, Vazquez JM, Martinez EA, Roca J (2008): Evaluation of L-glutamine for cryopreservation of boar spermatozoa. Anim Reprod Sci 115:149- 157.
21 Salisbury GW, Van Demark NL, Lodge JR (1978):Physiology of Reproduction and Artificial Insemination of Cattle. W. H. Freeman and Co. 2nd (eds), San Francisco, USA.
22 Holt WV, North RD (1984): Partially irreversible cold-induced lipid phase transitions in mammalian sperm plasma membrane domains : freeze-fracture study. J Exp Zool 230:473-483.   DOI   ScienceOn
23 Purdy PH, Graham JK (2004): Effect of cholesterolloaded cyclodextrin on the cryosurvival of bull sperm. Cryobiology 48:36-45.   DOI   ScienceOn
24 Neild DM, Gadella BM, Maria GC, Marcelo H, Miragaya C, Colenbrander B, Aguero A (2003): Membrans changes during different stages of a freezethaw protocol for equine semen cryopreservation. Theriogenology 59:1693-1705.   DOI   ScienceOn
25 Chiu PC, Chung MK, Tsang HY, Koistinen R, Koistinen H, Seppala M, Lee KF and Yeung WS (2005): Glycodelin-S in human seminal plasma reduces cholesterol effux and inhibits capacitation of spermatozoa. J Biol Chem 280:25580-25589.   DOI
26 Morrier A, The riault M, Castonguay F, Bailey JL (2004): Effect of cholesterol loaded methyl-$\beta$-cyclodextrin on ram sperm during cryopreservation, coldshock and artificial insemination. Page 239 (abstract 636) in Proc. Soc. Study of Reproduction Mtg., Vancouver, BC, Canada. Biology of Reproduction, Madison, WI.
27 Moore AI, Squires EL, Graham JK (2005): Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival. Cryobiology 51:241-249.   DOI   ScienceOn