Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Comparison of characteristics of xylitol-sensitive and xylitol-resistant Streptococcus mutans by use of various carbohydrates

다양한 탄수화물 분해에 따른 xylitol-sensitive Streptococcus mutans와 xylitol-resistant Streptococcus mutans의 특성 비교

  • Kim, Ji-Hye (Dept. of Preventive Dentistry, School of Dentistry, Kyungpook National University) ;
  • Lee, Young-Eun (Dept. of Dental Hygiene, Daegu Health College) ;
  • Ahn, Sang-Hun (Dept. of Dental Hygiene, Daegu Health College) ;
  • Choi, Youn-Hee (Dept. of Preventive Dentistry, School of Dentistry, Kyungpook National University) ;
  • Song, Keun-Bae (Dept. of Preventive Dentistry, School of Dentistry, Kyungpook National University)
  • 김지혜 (경북대학교 치의학전문대학원 예방치과학교실) ;
  • 이영은 (대구보건대학 치위생과) ;
  • 안상헌 (대구보건대학 치위생과) ;
  • 최연희 (경북대학교 치의학전문대학원 예방치과학교실) ;
  • 송근배 (경북대학교 치의학전문대학원 예방치과학교실)
  • Received : 2011.08.22
  • Accepted : 2011.10.06
  • Published : 2011.10.31
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Abstract

The aim of this study was to investigate the capacity of the xylitol-sensitive(Xs) and xylitol-resistant(Xr) S. mutans to induce dental caries in the presence of various carbohydrate. S. mutans KCTC3065 was cultured with 0.4% glucose and 1% xylitol in TYE medium for 30 days at $37^{\circ}C$, 10% $CO_2$ to form Xr S. mutans. Both Xs and Xr strains were cultured in four different carbohydrate environments; 0.5% glucose containing basal culture TYE medium(G-TYE), G-TYE plus 0.5% sucorse, G-TYE plus 0.5% fructose, G-TYE plus 0.5% maltose. Then cell growth, acid production, and extracellular polysaccharides synthesis were analyzed. The final growth level and extracellular polysaccharides contents in the Xr strain were significantly lower than in the Xs strain in all carbohydrates except fructose. While, acid production was no significantly difference between Xs and Xr strain. These results indicate that the virulence of Xr strains is significantly lower than that of Xs strains, which supports Xr strains may be less cariogenic than Xs strains.

본 연구는 다양한 탄수화물 아래에서 자일리톨 감성균주와 내성균주의 치아우식과 관련된 특성을 비교해보고자 하였다. 이를 위하여 1% 자일리톨이 첨가된 TYE 배지에서 S. mutans를 30일간 배양하여 자일리톨 내성균주를 형성하였으며, 이후 0.5% 포도당이 포함된 배지에 자당, 과당, 엿당을 첨가하여 성장, 산생성, 세포외부 다당류 합성정도를 평가하였다. 자일리톨 내성균주는 자일리톨 감성균주에 비해 과당을 제외한 모든 탄수화물 상에서 최종 성장이 유의하게 낮았으며(p<0.05), 산생성에는 유의한 차이가 없었다(p>0.05). 자일리톨 내성균주의 세포외부 다당류 합성은 과당을 제외한 모든 탄수화물 상에서 대체로 자일리톨 감성균주에 비해 낮았으며, 특히 엿당 상에서 뚜렷하게 낮았다. 이상의 결과들을 종합해 보았을 때, 자일리톨 감성균주와 내성균주의 독력인자 발현 정도는 탄수화물의 종류에 따라 차이를 보이기는 하나, 자일리톨 내성균주의 독력이 자일리톨 감성균주에 비해 대체로 낮으므로, 자일리톨의 장기적인 섭취에 의해 형성된 구강 내 자일리톨 내성균주는 자일리톨 감성균주에 비해 치아우식예방에 더 유리할 것으로 생각된다.

Keywords

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