Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Availability of CLSI method and MicroScan MICroSTREP plus panel for Antimicrobial Susceptibility Testing of α-hemolytic streptococci Isolated from Patients

환자(患者)에서 분리한 연쇄상구균(連鎖狀球菌)에 대한 CLSI방법(方法)과 MicroScan MICroSTREP plus panel의 항균제(抗菌劑) 감수성(感受性) 검사(檢査)의 유용성(有用性) 고찰(考察)

  • Kim, Sang-Ha (Department of Public Health and Welfare Graduate school of Konyang University) ;
  • Kim, Sang-Ha (Department of Laboratory Medicine of Sangju Hospital, The Republic of National Red Cross) ;
  • Kim, Young-Kwon (Department of Biomedical Laboratory Science of Konyang University)
  • 김상하 (건양대학교병원 진단검사의학과) ;
  • 김상하 (대한적십자사상주병원 진단검사의학과) ;
  • 김영권 (건양대학교 임상병리학과)
  • Received : 2011.08.23
  • Accepted : 2011.11.10
  • Published : 2011.11.30
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Abstract

The purpose of this study was availability for the classical test method. The test were called CLSI(Clinical and Laboratory Standards Institute) that was disk diffusion method, the newly designed E-test(made use disk diffusion method) can estimate the MIC and modified broth microdilution method that was standardized. Those tests were observed by MicroScan MicroSTREP plus panel. Target strains were 53 strains of S.pneumoniae and 51 strains of ${\alpha}$-hemolytic streptococci which were separated from the inpatient in university hospital for 6 months from February to August, 2009. The 9 antimicrobial agent of target evaluation were cefotaxime, chloramphenicol, clindamycin, erythromycin, levofloxacin, penicillin, tetracycline, trimethoprim/sulfamethoxazole, and vancomycin. researched comparative analysis both S.pneumoniae and ${\alpha}$-hemolytic streptococci. The result of the high concordance rates in ${\alpha}$-hemolytic streptococci was recognized formally in clinical microbiology laboratory.

본 연구에서는 전 세계적으로 표준화된 전통적 항균제 감수성 검사방법인 CLSI(C linical and Laboratory Standards Institute)디스크 확산법과 디스크 확산법을 이용 해 MIC를 측정할 수 있도록 새롭게 고안된 E-test 방법과 최근 도입된 미량 액체 배지 희석법을 이용한 항균제 감수성 검사 자동화 장비인 MicroScan MICroSTR EP plus panel를 이용한 MIC 측정방법들의 일치도를 비교하여 MicroScan MICroS TREP plus panel 검사방법의 유용성을 관찰하고자 하였다. 대상균주는 2009년 2월부터 8월까지 6개월 동안 대전의 한 대학병원 임상환자에서 분리된 폐렴사슬알균 53균주와 알파용혈 사슬알균 51균주를 대상으로 하였으며 항 균제는 cefotaxime, chloramphenicol, clindamycin, erythromycin, levofloxacin, penicillin, tetracycline, trimethoprim/sulfamethoxazole, vancomycin 등 9가지 항균제를 평가 대상으로 하였다. 폐렴사슬알균 뿐만 아니라 알파용혈 사슬균에 대해서도 비교분석을 하였고 그 결과 알파용혈 사슬균에 대해 높은 일치율이 관찰되어 임상미생물 검사실에서 신속 정확한 최소발율 억제농도를 측정하는데 많은 도움이 되리라 생각된다.

Keywords

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