Browse > Article
http://dx.doi.org/10.5762/KAIS.2011.12.11.4951

Availability of CLSI method and MicroScan MICroSTREP plus panel for Antimicrobial Susceptibility Testing of α-hemolytic streptococci Isolated from Patients  

Kim, Sang-Ha (Department of Public Health and Welfare Graduate school of Konyang University)
Kim, Sang-Ha (Department of Laboratory Medicine of Sangju Hospital, The Republic of National Red Cross)
Kim, Young-Kwon (Department of Biomedical Laboratory Science of Konyang University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.12, no.11, 2011 , pp. 4951-4958 More about this Journal
Abstract
The purpose of this study was availability for the classical test method. The test were called CLSI(Clinical and Laboratory Standards Institute) that was disk diffusion method, the newly designed E-test(made use disk diffusion method) can estimate the MIC and modified broth microdilution method that was standardized. Those tests were observed by MicroScan MicroSTREP plus panel. Target strains were 53 strains of S.pneumoniae and 51 strains of ${\alpha}$-hemolytic streptococci which were separated from the inpatient in university hospital for 6 months from February to August, 2009. The 9 antimicrobial agent of target evaluation were cefotaxime, chloramphenicol, clindamycin, erythromycin, levofloxacin, penicillin, tetracycline, trimethoprim/sulfamethoxazole, and vancomycin. researched comparative analysis both S.pneumoniae and ${\alpha}$-hemolytic streptococci. The result of the high concordance rates in ${\alpha}$-hemolytic streptococci was recognized formally in clinical microbiology laboratory.
Keywords
S.pneumoniae 53 strains; ${\alpha}$-hemolytic streptococci 51 strains; CLSI;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Woo HY, Nam MH, LEE NY: Streptococcus pn eumoniae antimicrobial susceptibility testing of the VITEK-2 System for the assessment. Journ al of Clinical Pathology 2001; 21: 129-34
2 Antimicrobial susceptibility testing of fungal pn eumonia known for evaluation of the Phoenix System. Department of Laboratory Medicine 20 09 :29:212-7.
3 Klugman, K. P. 1990. Pneumococcal resistance to antibiotics. Clin. Microbiol. Rev1990;3:171-96.   DOI
4 LEE SH, KIM YS, Woo JH, Ryu JS, Bae JH, Cephalosporin Resistance 2 cases of meningitis caused by Streptococcus pneumoniae. Journal of Clinical Pathology 1997; 17: 629-35
5 Lee H, Yong D, Lee K, Hong SG, Kim EC, Jeong SH, et al. Antimicrobial resistance of clinically important bacteria isolated from 12 hospitals in Korea in 2004. Korean J Clin Microbiol 2005;8:66-73.
6 SONG JH, Jeong SI, Go GS, KIM NY, SON JS of Asia for some areas of pneumococcal antibio tic resistance patterns, and change status. Soci ety of Infection and Chemotherapy 2004; 48:2101-7.
7 Shin JH, Jung HJ, Kim HR, Jeong J, Jeong SH, Kim S, Lee EY, Lee JN,Ch ang CL. Prevalence, characteristics, and molecular epidemiology of macrolide and fluoroquinolone resistance in clinical isolates of Streptococcus pneumoni ae at five tertiary-care hospitals in Korea. Antimicrob Agents Chemother 51:2625-7, 2007   DOI
8 Treatment Guidelines for Community-acquired Pneumonia in Korea: An Evidence-based Approach to Appropriate Antimicrobial. Therapy. DOI : 10.3947/ic. 2009.41.3.133   DOI
9 Song JH, Oh WS, Kang CI, Chung DR, Peck KR, Ko KS, et al. Epidemiolgy and clinical outcomes of community-acquired pneumonia in adult patients in Asian countries: a prospective study by the Asian network for surve illance of resistant pathogens. International Journal of Antimicrobial Agents 2008;31:107-14.   DOI
10 Diekema DJ, Beach ML, Pfaller MA, Jones RN; SENTRYP articipants Group. Antimicrobial resistance in viridans group streptococci among patients with and without the diagnosis of cancer in the USA, Canada and Latin Amer ica. Clin Microbiol Infect 2001;7:152-7.   DOI
11 Kim HS, Kim JS, Ha CK, Song W, Lee KM. Evaluation of MicroScanMICro STREP Plus Antimicrobial susceptibility panel for testing Streptococcus pneumoniae. Korean J Clin Microbiol 2008;11:18-22.   DOI
12 LEE HD, Yong DE, LE KW, Hong SG, KIM UJ, Jeong SH. 2004, 12 hospitals in Korea sep arated from the main bacterial antibiotic naese ongryul. Journal of Clinical Microbiology 2005;8:66-73).
13 Musher DM. Infections caused by Streptococcus pneumoniae :clinical spectrum, pathogenesis, immunity and treatment. Clin Infect Dis 1992, 14:801-7.   DOI
14 Clinical and Laboratory Standards Institute. Performance standards for anti microbial susceptibility testing. Eighteenth informational supplement, M100-S18. Wayne, PA: Clinical and Laboratory Standards Institute, 2008.
15 Jorgensen JH, McElmeel ML, Crawford SA. Evaluation of the Dade MicroScan MICroSTREP antimicrobial susceptibility testing panel with selected Strep tococcus pneumoniae challenge strains and recent clinical isolates. J Clin Microbiol 1998;36:788-91.
16 Reichler, M. R., A. A. Allphin, R. F. Breiman, J.R. Schreiber, J. E. Arnold, L. K. McDougal, R. R. Facklam, B. Boxerbaum, D. May, R. O. Walton, and M R. Jacobs. 1992. The spread of multiply resistant Streptococcus pneumoniae at a day care center in Ohio. J. Infect. Dis. 166:1346-1353   DOI
17 Kim KH, Sohn YM, Kang JH, Kim KN, Kim DS, Kim JH, et al. The causa tive organisms of bacterial meningitis in Korean children, 1986-1995. J Kore an Med Sci 1998;13: 60-4.   DOI
18 Wilson WR, Karchmer AW, Dajani AS, Taubert KA, Bayer A, Kaye D, et al. Antibiotic treatment of adults with infective endocarditis due to streptococci, enterococci, staphylococci, and HACEK microorganisms. JAMA 1995; 274:1706-13.   DOI
19 Bochud PY, Calandra T, Francioli P. Bacteremia due to viridans streptococci in neutropenic patients: a review. Am JMed 1994;97:256-64.
20 Ruoff KL, Whiley RA, Beighton D.Streptococcus. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, eds. Manual of Clinical Microbiol ogy.7th ed.Washington DC: American Society for Microbiology, 1999: 283-96.
21 Heo JW, Na J, LEE SH, bae JH, Streptococcus pneumoniae penicillin and cefotaxim susceptibility of E-test the usefulness of a search Corporation. Journal of Clinical Pathology 1996; 16:33 0-9.
22 Lalitha MK, Manayani DJ, Priya L, Jesu dason MV, Thomas K, Steinhoff MC. E test as an alternative to conventional MIC determination for surveillance of drug resistant Streptococcus pneumoniae. Indian JMed Res1997;10 6:500-3.
23 Spellerberg B and Brandt C. Streptococcus. In: Murray PR, Baron EJ, et aleds. Manual of clinical microbiology. 9th ed. Washington DC: ASM press, 2007:412-25.