Journal of Korean Society of Occupational and Environmental Hygiene (한국산업보건학회지)

Korean Industrial Hygiene Association (한국산업보건학회)
권호 표지

Levels and Related Factors of Airborne Fungi in Microbial and Chemistry Laboratories in Universities

일부 대학교 미생물실험실 및 화학실험실에서의 진균 분포 및 관련인자

  • Hwang, Sung-Ho (Department of Environmental Health, School of Public Health and Institute of Health and Environment, Seoul National University) ;
  • Jo, Hyun-Woo (Department of Environmental Health, School of Public Health and Institute of Health and Environment, Seoul National University) ;
  • Park, Dong-Uk (Department of Environmental Health, Korea National Open University) ;
  • Yoon, Chung-Sik (Department of Environmental Health, School of Public Health and Institute of Health and Environment, Seoul National University) ;
  • Ryu, Kyong-Nam (Department of Environmental Health, School of Public Health and Institute of Health and Environment, Seoul National University) ;
  • Ha, Kwon-Chul (Department of Biochemistry and Health Science, Changwon National University)
  • 황성호 (서울대학교 보건대학원 환경보건학과, 보건환경연구소) ;
  • 조현우 (서울대학교 보건대학원 환경보건학과, 보건환경연구소) ;
  • 박동욱 (한국방송통신대학교 환경보건학과) ;
  • 윤충식 (서울대학교 보건대학원 환경보건학과, 보건환경연구소) ;
  • 류경남 (서울대학교 보건대학원 환경보건학과, 보건환경연구소) ;
  • 하권철 (창원대학교 보건의과학과)
  • Received : 2009.11.25
  • Accepted : 2010.03.15
  • Published : 2010.03.30
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Abstract

The purpose of this study is to assess the level of fungi concentration in the university laboratories in Seoul, Korea, and to investigate factors contributing to these concentrations. The samples were taken from three spots in each laboratory; the top of sink, the center of laboratory, and the front of ventilation system, i.e fume hood at the chemical laboratory and clean bench/biosafety cabinet at the microbial laboratory. Air samples were collected using the single-stage Anderson sampler (Quick Take 30) at a flow rate of 28.3 l/min for 5 min on nutrient media in Petri-dishes located on the impactor. Fifty-two air samples were collected from 19 different laboratories (13 microbiology laboratories, 6 chemistry laboratories) in the university, and concentrations of airborne fungi showed no significant difference (p>0.05) between microbiology and chemistry laboratory, and also no significant difference at three locations (sink, center, front of ventilation system) in microbiology and chemistry laboratories. Average concentrations of fungi in 19 laboratories ranged from 7 to 459 cfu/$m^3$, with an overall Geometric Mean of 52 cfu/$m^3$. Airborne fungi concentrations of 6 samples (12 %) exceeded 150 cfu/$m^3$, the guideline of WHO. The ratios of Indoor/Outdoor for airborne fungi ranged from 0.2 to 4.8 (mean = 1.6). Related factors were measured such as relative humidity, temperature, and laboratory area. Temperature and laboratory area showed no significant relations to concentrations of airborne fungi except for relative humidity in the laboratory Concentrations of fungi were significant different (p<0.01) between rainy or cloudy and sunny. However, there was no significant difference between general ventilation and nongeneral ventilation.

Keywords

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