한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제33권2호
  • /
  • Pages.199-206
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  • 2010
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지

Nano powder propolis 투여가 streptozotocin으로 유발된 당뇨병 rat의 혈당에 미치는 영향

Hypoglycemic effects of nano powder propolis on streptozotocin-induced diabetic rats

  • 투고 : 2010.04.20
  • 심사 : 2010.06.10
  • 발행 : 2010.06.30
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초록

To evaluate the hypoglycemic effect of nano powder propolis, streptozotocin (STZ) induced diabetic rats were divided into 2 groups : diabetic control group and nano powder propolis (0.9ml) group. Then the rats were fed with nano-powder-propolis for 4 weeks. After 4 weeks, oral glucose tolerance test (oral GTT) was performed and blood sugar levels after 16 hours fasting, body weights, and blood lipid levels were measured. Finally, pancreas were collected and examined by histopathology and immunohistochemistry. In conclusion, the nano-powder-propolis was effective in the treatment of diabetes due to the reduction of blood sugar level and the regeneration of damaged ${\beta}$-cells observed in streptozotocin-induced diabetic rats.

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참고문헌

  1. 김희성, 이동배, 조영채, 정년기, 하창수. 2008. Nano Emulsion 기술을 이용한 Propolis Nano Powder 제조기술 개발. 지식경제부. (주)가보팜스 연구보고서: 1-72.
  2. 송효남. 2006. Propolis 수용성 분말 제조 및 이를 첨가한 빵의 저장 중 품질변화. 한국식품조리과학회 22(6): 905-913.
  3. 오근영. 1987. 조직병리학실기. 대학서림, 서울: 90-92.
  4. 이동배, 조영채, 정년기. 2004. 무알코올 수용성 프로폴리스의 제조방법과 질병효능실험. (주)가보팜스, 충남대학교 연구보고서: 1-18.
  5. 정년기, 이동배, 조영채, 하창수. 2005. Propolis 투여가 Streptozotocin으로 유발된 당뇨병 rat의 혈당에 미치는 영향. 한국환경보건학회지 31(1): 47-54.
  6. 한충택. 2003. HPLC에 의한 프로폴리스의 성분 분석 및 Streptozotocin 당뇨 유발 흰쥐에서의 수용성 프로폴리스의 항당뇨 효과. 강원대학교 대학원(박사학위논문).
  7. 허용갑. 2006. 국산 프로폴리스를 이용한 무알콜 수용성의 기능성 식품 개발과 임상 실험을 통한 항산화 및 면역증강효과의 검증. 농림부. 서울프로폴리스(주) 연구보고서: 1-149.
  8. Beukelman CJ, De Vries PJF, Schaafsma A, Quaries van Ufford HC, Keuenen J, Kroes BH, Van den Worm E, van den Berg AJJ, Labadie RP, van Dijk R. 1997. Immunomodulating properties of propolis. Pharm Pharmacol Lett 7(2/3): 75-77.
  9. Cardinal JW, Margison GP, Mynett KJ, Yates AP, Cameron DP, Elder RH. 2001. Increased susceptibility to streptozotocin-induced beta-cell apoptosis and delayed autoimmune diabetes in alkylpurine-DNA-N-glycosylase- deficient mice. Mol Cell BioI 21 (16): 5605-5613. https://doi.org/10.1128/MCB.21.16.5605-5613.2001
  10. Erhan Eroglu H, Ozkul Y, Tatlisen A, Silici S. 2008. Anticarcinogenic and antimitotic effects of Turkish propolis and mitomycin-Con tissue cultures of bladder cancer. Nat Prod Res 22(12): 1060-1066. https://doi.org/10.1080/14786410802263972
  11. Fuliang HU, Hepburn HR, Xuan H, Chen M, Daya S, Radloff SE. 2005. Effects of propolis on blood glucose, blood lipid and free radicals in rats with diabetes mellitus. Pharmacol Res 51(2): 147-152. https://doi.org/10.1016/j.phrs.2004.06.011
  12. Gomez-Romero M, Arraez-Roman D, Moreno-Torres R, Garcia-Salas P, Segura-Carretero A, Femandez-Gutierez A. 2007. Antioxidant compounds of propolis determined by capillary electrophoresis-mass spectrometry. J Sep Sci 30(4): 595-603. https://doi.org/10.1002/jssc.200600354
  13. Grange JM, Davey RW. 1990. Antibacterial properties of propolis (bee glue). J R Soc Med 83(3): 159-160.
  14. Greenaway W, May J, Scaysbrook T, Whatley FR. 1991. Identification by gas Chromatography-mass spectrometry of 150 compounds in propolis. Z Naturforsch 46(C): 111-121.
  15. Hausen BM, Wollenweber E, Senff H, Post B. 1987. Propolis allergy. (I). Origin, properties, usage and literature review. Contact Dermatitis 17(3): 163-170. https://doi.org/10.1111/j.1600-0536.1987.tb02699.x
  16. Junod A, Lambert AE, Orci L, Pictet R, Gonet AE, Renold AE. 1967. Studies of the diabetogenic action of streptozotocin. Proc Soc Exp BioI Med 126(1): 201-205.
  17. Kanbur M, Eraslan G, Silici S. 2009. Antioxidant effect of propolis against exposure to propetamphos in rats. Ecotoxicol Environ Saf 72(3): 909-915. https://doi.org/10.1016/j.ecoenv.2007.12.018
  18. Liu H. 2005. Nano-propolis a new kind of antibacterial agents. Food Res Develop 26(6): 107-109 .
  19. Lotfy M, Badra G, Burham W, Alenzi FQ. 2006. Combined use of honey, bee propolis and myrrh in healing a deep, infected wound in a patient with diabetes mellitus. Br J Biomed Sci 63(4): 171-173.
  20. Matsui T, Ebuchi S, Fujise T, Abesundara KJ, Doi S, Yamada H, Matsumoto K. 2004. Strong antihyperglycemic effects of water-soluble fraction of Brazilian propolis and its bioactive constituent, 3,4,5-tri-O-caffeoylquinic acid. BioI Pharm Bull 27(11): 1797-1803. https://doi.org/10.1248/bpb.27.1797
  21. Matsushige K, Basnet P, Hase K, Kadota S, Tanaka S, Namba T. 1996. Propolis protects pancreatic $\beta$-cells against the toxicity of streptozotocin (STZ). Phytomedicine 3(2): 203-209. https://doi.org/10.1016/S0944-7113(96)80037-7
  22. Murata K, Yatsunami K, Fukuda E, Onodera S, Mizukami O, Hoshino G, Kamei T. 2004. Antihyperglycemic effects of propolis mixed with mulberry leaf extract on patients with type 2 diabetes. Altern Ther Health Med 10(3): 78-79.
  23. Monti M. Berti E. Carminati G. Cusini M. 1983. Occupational and cosmetic dermatitis from propolis. Contact Dermatitis 9(2): 163-164.
  24. Park EH, Kim SH, Park SS. 1996. Anti-inflammatory activity of propolis. Arch Pharm Res 19(5): 337-341. https://doi.org/10.1007/BF02976375
  25. Sabu MC, Smitha K, Kuttan R. 2002. Anti-diabetic activity of green tea polyphenols and their role in reducing oxidative stress in experimental diabetes. J Ethnopharmacol 83(1-2): 109-116. https://doi.org/10.1016/S0378-8741(02)00217-9
  26. Said MM, Abedel-Aziz SF. 1990. Some biological aspects of propolis (bee-glue). 2-propolis as hypoglycemic agent. J Drug Res (Egypt) 19: 245-53
  27. Schnitzler P, Neuner A, Nolkemper S, Zundel C, Nowack H, Sensch KH, Reichling J. 2010. Antiviral activity and mode of action of propolis extracts and selected compounds. Phytother Res 24 (Suppl 1): S20-28. https://doi.org/10.1002/ptr.2868
  28. Silici S, Koc NA, Ayangil D, Cankaya S. 2005. Antifungal activities of propolis collected by different races of honeybees against yeasts isolated from patients with superficial mycoses. J Pharmacol Sci 99(1): 39-44. https://doi.org/10.1254/jphs.FPE05002X
  29. Villonueva VR, Barbier M, Gonnet M. Lavie P. 1970. Les flavonoides de la propolis. Isolement d'une nouvelle substance bacterioststique. la pinocembrine, Annals Inst, Pasteur. Paris 118: 84-87.
  30. Penelope W. Crane E. 1987. Constituents of propolis. Apidologie 18(4): 327-334. https://doi.org/10.1051/apido:19870404
  31. Zamami Y, Takatori S, Koyama T, Goda M, Iwatani Y, Doi S, Kawasaki H. 2007. Effect of propolis on insulin resistance in fructose-drinking rats. Yakugaku Zasshi 127(12): 2065-2073. https://doi.org/10.1248/yakushi.127.2065
  32. 高海琳, 孟培德 馬益嬌. 柳富海. 許止鼎. 廉所梅 2002. 峰膠在糖尿病綜合治療中的作用. 北京大學 中文核心基幹要目 總攬 2: 1-3.
  33. 董捷, 閻繼紅, 孫麗萍. 2003. 蜍膠複合軟膠褒降糖作用的實驗研究, 養蜂科技 5: 2-4.
  34. 王南舟. 2004. 蜂膠制濟對實驗性糖尿病免血糖的影響. 中國獻醫雜誌 40(4): 7-9.
  35. 胡福良, 玄紅專. 2003. 蜂膠對糖尿病鼠血糖的影響. 蜜蜂雜誌 10: 6-8.
  36. 胡福良, 玄紅專, 陳民利, 應和忠, 朱滅. 2004a. 蜂膠對糖尿病 SD大鼠的 影響. 浙江大學學報 30(2): 205-209.
  37. 胡福良, 玄紅專, 耀詹鋒. 2004b. 蜂膠對糖尿病大鼠蛋白質代射的 影響. 養蜂科技 1:2-3.
  38. 胡福良, 玄紅專, 耀詹鋒. 2004c. 蜂膠對糖尿病大鼠腎臟的 影響. 蜜蜂雜誌 2:3-4.