The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Simple and Reliable DNA Extraction Method for the Dark Pigmented Fungus, Cercospora sojina

  • Kim, Ji-Seong (Department of Environmental Horticulture, The University of Seoul) ;
  • Seo, Sang-Gyu (Department of Environmental Horticulture, The University of Seoul) ;
  • Jun, Byung-Ki (Department of Environmental Horticulture, The University of Seoul) ;
  • Kim, Jin-Won (Department of Environmental Horticulture, The University of Seoul) ;
  • Kim, Sun-Hyung (Department of Environmental Horticulture, The University of Seoul)
  • 투고 : 2010.07.18
  • 심사 : 2010.08.08
  • 발행 : 2010.09.01
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초록

This study used a modified cetyltrimethylammonium bromide (CTAB) method to efficiently extract DNA from the plant pathogenic fungus Cercospora sojina. Total DNA yield obtained by this method was approximately 1 mg/g of mycelia (fresh weight), and the mean ratio of A260/A280 and A260/A230 were 2.04 and 2.1, respectively. The results of random amplified polymorphic DNA (RAPD) analysis, digestion with restriction enzymes, and Southern hybridization indicated that polysaccharides were effectively removed by this method, and the resulting DNA was sufficient for use in subsequent molecular analysis.

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참고문헌

  1. Asakura, T., Adachi, K. and Schwartz, E. 1978. Stabilizing effectof various organic solvents on protein. J. Biol. Chem. 25:6423-6425.
  2. Chi, M. W., Park, S. Y. and Lee, Y. H. 2009. A quick and safemethod for fungal DNA extraction. Plant Pathol. J. 25:108-111. https://doi.org/10.5423/PPJ.2009.25.1.108
  3. Daub, M. E. and Chung, K. R. 2007. Cercosporin: a photoactivatedtoxin in plant disease. APSnet Feature Story. Februarywww.apsnet.org
  4. Farkas, V. 1979. Biosynthesis of cell walls of fungi. Microbiol.Reviews 43:117-144.
  5. Goodwin, S. B., Dunkle, L. D. and Zismann, V. L. 2001. Phylogeneticanalysis of Cercospora and Mycosphaerella based on theinternal transcribed spacer region of ribosomal DNA. Phytopathology91:648-658. https://doi.org/10.1094/PHYTO.2001.91.7.648
  6. Hartman, G. L., Sinclair, J. B. and Rupe, J. C. 1999. Frogeye leafspot. In: Compendium of soybean diseases, 4rd edn. TheAmerican Phytopathological Society Press. St Paul. pp. 20-21.
  7. Karakousis, A., Tan, L., Ellis, D., Alexiou, H. and Wormald, P. J.2006. An assessment of the efficiency of fungal DNA extractionmethods for maximizing the detection of medicallyimportant fungi using PCR. J. Microbiol. Methods 65:38-48. https://doi.org/10.1016/j.mimet.2005.06.008
  8. Kim, S. H. and Hamada, T. 2005. Rapid and reliable method ofextracting DNA and RNA from sweetpotato, Ipomoea batatas(L). Lam. Biotechnol. Lett. 27:1841-1845. https://doi.org/10.1007/s10529-005-3891-2
  9. Kuhad, R. C., Kapoor, R. K. and Lal, R. 2004. Improving theyield and quality of DNA isolated from white-rot fungi. FoliaMicrobiol. 49:112-116. https://doi.org/10.1007/BF02931383
  10. Loeffler, J., Hebart, H., Shumacher, U., Reitze, H. and Einsele, H.1997. Comparison of different methods for extraction of DNAof fungal pathogens from cultures and blood. J. Clin. Microbiol.35: 3311-3312.
  11. Mahuku, G. S. 2004. A simple extraction method suitable for PCRbased analysis of plant, fungal, and bacterial DNA. Plant Mol.Biol. Rep. 22:71-81. https://doi.org/10.1007/BF02773351
  12. Manna, B., Gambhir, A. and Ghosh, P. 1996. Production andrheological characteristics of the microbial polysaccharide gellan.Lett. Applied Microbiol. 23:141-145.
  13. Moller, E. M., Bahnweg, G., Sandermann, H. and Geiger, H. H.1992. A simple and efficient protocol for isolation of highmolecular weight DNA from filamentous fungi, fruit bodies,and infected plant tissues. Nucleic Acids Res. 20:6115-6116. https://doi.org/10.1093/nar/20.22.6115
  14. Pandey, R. N., Adams, R. P. and Flournoy, L. E. 1996. Inhibitionof random amplified polymorphic DNAs (RAPDs) by plantpolysaccharides. Plant Mol. Biol. Rep. 14:17-22. https://doi.org/10.1007/BF02671898
  15. Sreenivasaprasad, S. 1999. Isolation of fungal nucleic acids. In R.Rapley (ed) The Nucleic Acid Protocols Handbook, HumanaPress. Totowa. pp 37-45.

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