The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Microwave Irradiation-assisted RNA Extraction from Woody Tissues for Plant Virus Detection

  • Duong, Thanh Van (Department of Biotechnology, Catholic University of Daegu) ;
  • Shin, Dong-Il (Department of Biotechnology, Catholic University of Daegu) ;
  • Park, Hee-Sung (Department of Biotechnology, Catholic University of Daegu)
  • 투고 : 2010.07.07
  • 심사 : 2010.08.03
  • 발행 : 2010.09.01
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초록

Plant tissue homogenization using a mortar or mechanical equipment has been the preferred method for obtaining high yields of total RNA; this method, however, is both time-consuming and expensive. Additionally, homogenization may generate excessive endogenous RNases, polyphenolics, and other substances that reduce the quality and quantity of RNA. In this study, we describe the microwave irradiation-assisted RNA extraction (MIRE) technique which, without tissue disruption and homogenization, allows for the cost-effective and rapid generation of intact RNA from apple cane shavings and the reliable detection of apple virus by RT-PCR.

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참고문헌

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피인용 문헌

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