[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5423/PPJ.2010.26.3.289

Simple and Reliable DNA Extraction Method for the Dark Pigmented Fungus, Cercospora sojina

Kim, Ji-Seong (Department of Environmental Horticulture, The University of Seoul)
Seo, Sang-Gyu (Department of Environmental Horticulture, The University of Seoul)
Jun, Byung-Ki (Department of Environmental Horticulture, The University of Seoul)
Kim, Jin-Won (Department of Environmental Horticulture, The University of Seoul)
Kim, Sun-Hyung (Department of Environmental Horticulture, The University of Seoul)

Publication Information

The Plant Pathology Journal / v.26, no.3, 2010 , pp. 289-292 More about this Journal

Abstract

This study used a modified cetyltrimethylammonium bromide (CTAB) method to efficiently extract DNA from the plant pathogenic fungus Cercospora sojina. Total DNA yield obtained by this method was approximately 1 mg/g of mycelia (fresh weight), and the mean ratio of A260/A280 and A260/A230 were 2.04 and 2.1, respectively. The results of random amplified polymorphic DNA (RAPD) analysis, digestion with restriction enzymes, and Southern hybridization indicated that polysaccharides were effectively removed by this method, and the resulting DNA was sufficient for use in subsequent molecular analysis.

Keywords

Cercospora sojina; DNA extraction; fungus; RAPD; Restriction enzyme;

Citations & Related Records

Times Cited By KSCI : 1 (Citation Analysis)
Times Cited By Web Of Science : 0 (Related Records In Web of Science)

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Cited By KSCI

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