YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Knock-down of Type 2 Inositol 1,4,5-Trisphosphate Receptors using Adenovirus in Adult Ventricular Myocytes

아데노바이러스를 이용한 성체 심실 근세포 이노시톨 1,4,5-삼인산 수용체 제 2 아형의 발현 억제

  • Received : 2009.08.26
  • Accepted : 2009.12.03
  • Published : 2010.02.28
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Abstract

Inositol 1,4,5-trisphosphate ($IP_3$) receptor ($IP_3R$)-mediated signaling pathway is involved in many cellular processes including fertilization, apoptosis and neuronal function. Although cardiac myocytes express the $IP_3R$, its pathophysiological role has not been clearly understood because of limited selectivity of currently available pharmacological blockers. In the present study we constructed shRNA-expressing adenovirus to knock-down the type 2 $IP_3R$ ($IP_3R2$), a major subtype in cardiac ventricular myocytes, and demonstrated that the virus successfully eliminated the expression and localization of the $IP_3R2$. These results may provide a reliable tool for probing pathophysiological roles of the $IP_3R2$ in isolated intact cardiac myocytes.

Keywords

References

  1. Berridge, M. J. : Inositol trisphosphate and calcium signaling. Nature 361, 315 (1993). https://doi.org/10.1038/361315a0
  2. Furuichi, T., Kohda, K., Miyawaki, A. and Mikoshiba, K. : Intracellular channels. Curr. Opin. Neurobiol. 4(3), 294 (1994). https://doi.org/10.1016/0959-4388(94)90089-2
  3. Gyorke, S. and Fill, M. : Ryanodine receptor adaptation; Control mechanism of $ca^{2+}$-induced $ca^{2+}$ release in heart. Science 260, 807 (1993). https://doi.org/10.1126/science.8387229
  4. Smith, J. S., Imagawa, T., Ma, J., Fill, M., Campbell, K. P. and Coronado, R. : Purified ryanodine receptor from rabbit skeletal muscle is the calcium-release channel of sarcoplasmic reticulum. J. Gen. Physiol. 92, 1 (1988). https://doi.org/10.1085/jgp.92.1.1
  5. Lipp, P., Laine, M., Tovey, S. C., Burrell, T. K., Berridge, M. J., Li, W. and Bootman, M. D. : Functional InsP3 receptors that may modulate excitation-contraction coupling in the heart. Curr. Biol. 10, 939 (2000). https://doi.org/10.1016/S0960-9822(00)00624-2
  6. Maruyama, T., Kanaji, T., Nakada, S., Kanno, T. and Mikoshiba, K. : 2APB, 2-amino ethoxydiphenyl borate, a membranepenetrable modulator of Ins(1,4,5)$p_3$-induced $ca^{2+}$ release. J. Biochem. 122, 498 (1997). https://doi.org/10.1093/oxfordjournals.jbchem.a021780
  7. Gregory, R. B., Rychkov, G. and Barritt, G. J. : Evidence that 2-aminoethyl diphenylborate is a novel inhibitor of storeoperated $ca^{2+}$ channels in liver cells, and acts through a mechanism which does not involve inositol trisphosphate receptors. J. Biochem. 354, 285 (2001). https://doi.org/10.1042/0264-6021:3540285
  8. Ma, H. T., Venkatachalam, K., Li, H. S., Montell, C., Kurosaki, T., Patterson, R. L. and Gill, D. L. : Assessment of the inositol 1,4,5-trisphosphate receptor in the activation of transient receptor potential channels and store-operated $ca^{2+}$ entry channels. J. Biol. Chem. 276(22), 18888 (2001). https://doi.org/10.1074/jbc.M100944200
  9. Prakriya, M. and Lewis, R. S. : Potentiation and inhibition of $ca^{2+}$ release-activated $ca^{2+}$ channels by 2-aminoethyldiphenyl borate (2-APB) occurs independently of $IP_3$ receptors. J. Physiol. 536, 3 (2007).
  10. Bishara, N. B., Murphy, T. V. and Hill, M. A. : Capacitative $ca^{2+}$ entry in vascular endothelial cells is mediated via pathways sensitive to 2 aminoethoxydiphenyl borate and xestospongin C. J. Br. Phamacol. 135, 119 (2002). https://doi.org/10.1038/sj.bjp.0704465
  11. De Smet, P., Parys, J. B., Callewaert, G., Weidema, A. F., Hill, E., De Smedt, H., Erneux, C., Sorrentino, V. and Missiaen, L. : Xestospongin C is an equally potent inhibitor of the inositol 1,4,5-trisphosphate receptor and the endoplasmicreticulum $ca^{2+}$ pumps. Cell Calcium. 26(1/2), 9 (1999). https://doi.org/10.1054/ceca.1999.0047
  12. Ehrlich, B. E., Kaftan, E., Bezprozvannaya, S. and Bezprozvanny, I. : The pharmacology of intracellular $ca^{2+}$-release channels. Trends. Pharmacol. Sci. 15(5), 145 (1994). https://doi.org/10.1016/0165-6147(94)90074-4
  13. Taylor, C. W. and Broad, L. M. : Pharmacologial analysis of intracellular $ca^{2+}$ signaling. Problems and pitfalls. TiPS 19, 370 (1998).
  14. Li, X., Zima, A. V., Sheikh, F., Blatter, L. A. and Chen, J. : Endothelin-1-induced arrhythmogenic $ca^{2+}$ signaling is abolished in atrial myocytes of inositol 1,4,5-trisphosphate ($IP_3$)-receptor type 2-deficient mice. Circ. Res. 1274 (2005).
  15. Woo, S. H., Cleemann, L. and Morad, M. : Ca2+ current-gated focal and local $ca^{2+}$ release in rat atrial myocytes: Evidence from rapid 2-D confocal imaging. J. Physiol. 543, 439 (2002). https://doi.org/10.1113/jphysiol.2002.024190
  16. Zhou, Y. Y., Wang, S. Q., Zhu, W. Z., Chruscinski, A., Kobilka, B. K., Ziman, B., Wang, S., Lakatta, E. G., Cheng, H. and Xiao, R. P. : Culture and adenoviral infection of adult mouse cardiac myocytes: Methods for cellular genetic physiology. Am. J. Physiol. Heart. Circ. Physiol. 279, H429 (2000) https://doi.org/10.1152/ajpheart.2000.279.1.H429
  17. Perez, P. J., Ramos-Franco, J., Fill, M. and Mignery, G. A. : Identification of functional reconstitution of the type 2 inositol 1,4,5-trisphosphate receptor from ventricular cardiac myocytes. J. Biol. Chem. 272(38), 23961 (1997). https://doi.org/10.1074/jbc.272.38.23961