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Knock-down of Type 2 Inositol 1,4,5-Trisphosphate Receptors using Adenovirus in Adult Ventricular Myocytes  

Son, Min-Jeong (College of Pharmacy, Chungnam National University)
Subedi, Krishna P. (College of Pharmacy, Chungnam National University)
Woo, Sun-Hee (College of Pharmacy, Chungnam National University)
Publication Information
YAKHAK HOEJI / v.54, no.1, 2010 , pp. 8-12 More about this Journal
Abstract
Inositol 1,4,5-trisphosphate ($IP_3$) receptor ($IP_3R$)-mediated signaling pathway is involved in many cellular processes including fertilization, apoptosis and neuronal function. Although cardiac myocytes express the $IP_3R$, its pathophysiological role has not been clearly understood because of limited selectivity of currently available pharmacological blockers. In the present study we constructed shRNA-expressing adenovirus to knock-down the type 2 $IP_3R$ ($IP_3R2$), a major subtype in cardiac ventricular myocytes, and demonstrated that the virus successfully eliminated the expression and localization of the $IP_3R2$. These results may provide a reliable tool for probing pathophysiological roles of the $IP_3R2$ in isolated intact cardiac myocytes.
Keywords
inositol 1,4,5-trisphosphate receptor ($IP_3R$); adenoviral knock-down; adult ventricular myocytes;
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