한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제18권4호
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  • Pages.224-230
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  • 2010
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

고지방 식이로 유도된 비만 흰쥐의 간에서 식이섬유와 녹차혼합물의 항산화 및 항염증 효과

Anti-Oxidative and Anti-Inflammatory Effects of Green Tea Mixture and Dietary Fiber on Liver of High Fat Diet-Induced Obese Rats

  • 김종대 (강원대학교 의료바이오신소재 융복합연구 사업단) ;
  • 이병일 (강원대학교 의료바이오신소재 융복합연구 사업단) ;
  • 전윤희 (건국대학교 의료생명대학 생명과학부) ;
  • 박종필 (건국대학교 의료생명대학 생명과학부) ;
  • 김해란 (건국대학교 의료생명대학 생명과학부) ;
  • 임병우 (건국대학교 의료생명대학 생명과학부)
  • Kim, Jong-Dai (The Regional Core Research Program/Medical & Bio-Material Research Center, Kangwon National University) ;
  • Lee, Byung-Il (The Regional Core Research Program/Medical & Bio-Material Research Center, Kangwon National University) ;
  • Jeon, Yun-Hui (Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk University) ;
  • Bak, Jong-Phil (Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk University) ;
  • Jin, Hai-Lan (Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk University) ;
  • Lim, Beong-Ou (Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk University)
  • 투고 : 2010.04.26
  • 심사 : 2010.07.28
  • 발행 : 2010.08.30
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초록

This study was conducted to investigate anti-oxidative and inflammatory inhibition effects of green tea and dietary fiber mixture on liver of high fat diet-induced obese rats. 21 male rats were divided into 3 dietary groups and control group (A), high fat diet-induced group (B), and high fat (HF) diet-induced + EQ diet-$S^{(R)}$ diet group (C). Immunoblotting and RT-PCR analysis revealed protein expression, and anti-oxidant analysis revealed MDA (malondialdehyde), GSH (glutathione), and free DPPH radical. As a results, Body weight and food consumption were not significantly different between groups. The levels of MDA and GSH were lower in HF + $EQS^{(R)}$ group than in HF group. Also, the $EQS^{(R)}$ demonstrated to be more effective than HF group for a DPPH radicals scavenging activities. In addition, protein and mRNA level of TNF-$\alpha$ in HF + $EQS^{(R)}$ group showed relatively more potent pro-inflammatory activity inhibition compared to HF group. These results suggest that green tea mixture (EQ diet-$S^{(R)}$) provide positive effects on anti-oxidative and inflammatory inhibition effects on obese animal model or obesity related diseases.

키워드

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