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Anti-Oxidative and Anti-Inflammatory Effects of Green Tea Mixture and Dietary Fiber on Liver of High Fat Diet-Induced Obese Rats  

Kim, Jong-Dai (The Regional Core Research Program/Medical & Bio-Material Research Center, Kangwon National University)
Lee, Byung-Il (The Regional Core Research Program/Medical & Bio-Material Research Center, Kangwon National University)
Jeon, Yun-Hui (Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk University)
Bak, Jong-Phil (Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk University)
Jin, Hai-Lan (Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk University)
Lim, Beong-Ou (Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk University)
Publication Information
Korean Journal of Medicinal Crop Science / v.18, no.4, 2010 , pp. 224-230 More about this Journal
Abstract
This study was conducted to investigate anti-oxidative and inflammatory inhibition effects of green tea and dietary fiber mixture on liver of high fat diet-induced obese rats. 21 male rats were divided into 3 dietary groups and control group (A), high fat diet-induced group (B), and high fat (HF) diet-induced + EQ diet-$S^{(R)}$ diet group (C). Immunoblotting and RT-PCR analysis revealed protein expression, and anti-oxidant analysis revealed MDA (malondialdehyde), GSH (glutathione), and free DPPH radical. As a results, Body weight and food consumption were not significantly different between groups. The levels of MDA and GSH were lower in HF + $EQS^{(R)}$ group than in HF group. Also, the $EQS^{(R)}$ demonstrated to be more effective than HF group for a DPPH radicals scavenging activities. In addition, protein and mRNA level of TNF-$\alpha$ in HF + $EQS^{(R)}$ group showed relatively more potent pro-inflammatory activity inhibition compared to HF group. These results suggest that green tea mixture (EQ diet-$S^{(R)}$) provide positive effects on anti-oxidative and inflammatory inhibition effects on obese animal model or obesity related diseases.
Keywords
High Fat; Green tea; MDA; GSH; DPPH; TNF-$\alpha$;
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