Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Safety Evaluation of Epimedium koreanum Water Extract in Sprague-Dawley Rats

음양곽 물추출물의 독성 평가

  • Kim, Joo-Wan (Department of Internal Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Lim, Mee-Kyung (Department of Food Science and Nutrition, College of Human Ecology, Kyungpook National University) ;
  • Kim, Hong-Tae (Busan Metropolitan City Institute of health & Environment) ;
  • Ku, Sae-Kwang (Department of Anatomy and histology, College of Daegu Haany University) ;
  • Chang, Hey-Sook (Department of Internal Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Oh, Tae-Ho (Department of Internal Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Keun-woo (Department of Internal Medicine, College of Veterinary Medicine, Kyungpook National University)
  • Accepted : 2010.04.08
  • Published : 2010.04.30
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Abstract

To evaluate of oral toxicity of Epimedium koreanum nakai (EKN) water extract, three doses of EKN water extract (10, 100 and 1000 mg/kg/day) were administered to healthy Sprague-Dawley rats for 4 weeks. The results showed that there are no abnormal signs during the experimental period. The weights of testis and epididymis were increased dose-dependently, but seminal vesicle was decreased compared with control group. In hematology and serum chemistry, changes were observed within the normal ranges. In histological finding, slight to moderated changes were found but those were commonly or rarely encountered in the normal rats. These results suggest that no observed adverse effect level (NOAEL) of the oral application of EKN was considered to be more than 1000 mg/kg in SD rats under the conditions employed in this study.

음양곽 물추출물의 랫에서의 안전성을 검증하기 위하여 그룹당 5마리씩 총 20마리의 수컷 SD 랫에 각각 음양곽 물추출물을 체중당 10, 100, 1000 mg/kg을 1일 1회 4주간 경구투여 하였다. 대조군으로는 생리식염수를 동량 투여하였다. 임상증상, 체중 및 사료섭취량, 혈액, 혈액생화학 및 조직학적 검사를 실시하였다. 시험기간 중 임상증상, 체중, 사료섭취량의 이상변화를 관찰할 수 없었으며, 투약종료 다음날 부검결과 고환, 부고환의 무게가 용량의존적으로 증가하였으나, 정낭선은 감소경향을 나타내었다. 조직학적 검사결과 일부 변화가 관찰되었으나 건강한 쥐에서도 발견 되는 변화였다. 따라서 체중당 1000 mg까지의 투여는 임상적으로 안전한 것으로 사료된다.

Keywords

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