Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Coculture of Bovine Chondrocytes with Demineralized Bone Matrix in Alginate Bead and Pellet Cultures

알긴산 배양과 펠렛 배양에서 소연골세포와 탈회골기질의 공배양

  • Sutradhar, Bibek Chandra (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Hong, Gyeong-Mi (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Park, Jin-Uk (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Choi, Seok-Hwa (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Gon-Hyung (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University)
  • Accepted : 2010.03.23
  • Published : 2010.04.30
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Abstract

Bio-integration of cartilage grafts with subchondral bone is a significant clinical challenge. To date, the use of demineralized bone matrix (DBM) has been one of the most effective strategies for bone cell proliferation in vivo. Here, we investigated whether coculture of chondrocytes and DBM could serve as a single-platform system containing all the essential elements for purposive bone and cartilage induction. The aim of this study was to evaluate and compare the phenotype and proliferation of bovine chondrocytes cocultured with DBM in two different culture systems, pellet and alginate bead culture. In alginate bead culture, we observed an increase in chondrocyte number and formation of cell clusters. Typical chondrocytic phenotype was maintained for entire eight weeks. Histological analysis showed that chondrocytes maintained a typical round, plump morphology and there was a gradual increase in lacunae. Both coculture systems yielded an expanded cell population as compared to the controls (chondrocytes alone). The production of glycosaminoglycans was also increased in the coculture systems as compared to controls.

연골밑뼈와 연골이식편 사이의 생유합성은 임상적으로 중요한 과제이다. 현재까지 탈회골기질의 이용은 생체내 뼈세포 증식에 있어서 가장 효과적인 방법이다. 본 연구에서는 연골세포와 탈회골기질의 공배양을 통해 뼈와 연골의 유도 목적에 부합되는 모든 필수적인 요소를 갖는 재료로 이용가능 여부를 확인하고자 실시되었다. 본 연구의 목적은 두 종류의 배양법 즉, 펠렛 배양과 알긴산 배양에서 탈회골기질과 공배양된 소 연골세포의 증식과 표현형을 비교, 평가하는 것이다. 알긴산 배양에서는 세포 군집의 형성 및 연골세포의 수적 증가가 관찰되었다. 전형적인 연골세포의 표현형이 시험기간인 8주에 걸쳐 유지되었으며, 조직학적인 검사에서 연골세포는 일반적인 원형의 형태를 유지하였고, 연골세포방과 연골세포가 점진적으로 증가하였다. 대조군(연골세포 단독배양)에 비해 탈회골기질과 공배양한 두군 모두에서 많은 세포증식이 관찰되었으며, 글리코사미노글리칸의 생성 또한 증가되었다.

Keywords

References

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