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Coculture of Bovine Chondrocytes with Demineralized Bone Matrix in Alginate Bead and Pellet Cultures  

Sutradhar, Bibek Chandra (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University)
Hong, Gyeong-Mi (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University)
Park, Jin-Uk (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University)
Choi, Seok-Hwa (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University)
Kim, Gon-Hyung (Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University)
Publication Information
Journal of Veterinary Clinics / v.27, no.2, 2010 , pp. 147-153 More about this Journal
Abstract
Bio-integration of cartilage grafts with subchondral bone is a significant clinical challenge. To date, the use of demineralized bone matrix (DBM) has been one of the most effective strategies for bone cell proliferation in vivo. Here, we investigated whether coculture of chondrocytes and DBM could serve as a single-platform system containing all the essential elements for purposive bone and cartilage induction. The aim of this study was to evaluate and compare the phenotype and proliferation of bovine chondrocytes cocultured with DBM in two different culture systems, pellet and alginate bead culture. In alginate bead culture, we observed an increase in chondrocyte number and formation of cell clusters. Typical chondrocytic phenotype was maintained for entire eight weeks. Histological analysis showed that chondrocytes maintained a typical round, plump morphology and there was a gradual increase in lacunae. Both coculture systems yielded an expanded cell population as compared to the controls (chondrocytes alone). The production of glycosaminoglycans was also increased in the coculture systems as compared to controls.
Keywords
coculture; bovine chondrocytes; demineralized bone matrix;
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