Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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GENE EXPRESSION PROFILING IN HUMAN DENTAL PULP CELLS TREATED WITH MINERAL TRIOXIDE AGGREGATE

Mineral trioxide aggregate가 인간치수세포에서 유전자 발현에 미치는 영향

  • Kim, Yong-Beom (Department of Conservative Dentistry, Dental Research Institute, BK21 Program, School of Dentistry, Seoul National University) ;
  • Shon, Won-Jun (Department of Conservative Dentistry, Dental Research Institute, BK21 Program, School of Dentistry, Seoul National University) ;
  • Lee, Woo-Cheol (Department of Conservative Dentistry, Dental Research Institute, BK21 Program, School of Dentistry, Seoul National University) ;
  • Kum, Kee-Yeon (Department of Conservative Dentistry, Dental Research Institute, BK21 Program, School of Dentistry, Seoul National University) ;
  • Baek, Seung-Ho (Department of Conservative Dentistry, Dental Research Institute, BK21 Program, School of Dentistry, Seoul National University) ;
  • Bae, Kwang-Shik (Department of Conservative Dentistry, Dental Research Institute, BK21 Program, School of Dentistry, Seoul National University)
  • 김용범 (서울대학교 치의학대학원 치과보존학교실, 치의학연구소, BK21 Program) ;
  • 손원준 (서울대학교 치의학대학원 치과보존학교실, 치의학연구소, BK21 Program) ;
  • 이우철 (서울대학교 치의학대학원 치과보존학교실, 치의학연구소, BK21 Program) ;
  • 금기연 (서울대학교 치의학대학원 치과보존학교실, 치의학연구소, BK21 Program) ;
  • 백승호 (서울대학교 치의학대학원 치과보존학교실, 치의학연구소, BK21 Program) ;
  • 배광식 (서울대학교 치의학대학원 치과보존학교실, 치의학연구소, BK21 Program)
  • Received : 2010.03.20
  • Accepted : 2010.04.12
  • Published : 2010.05.31
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Abstract

This study investigated the changes in gene expression when mineral trioxide aggregate (MTA) was applied in vitro to human dental pulp cells (HDPCs). MTA in a teflon tube (diameter 10 mm, height 2 mm) was applied to HDPCs. Empty tube-applied HDPCs were used as negative control. For microarray analysis, total RNA was extracted at 6, 24, and 72 hrs after MTA application. The results were confirmed selectively by performing reverse transcriptase polymerase chain reaction for genes that showed changes of more than two-fold or less than half. Of the 24,546 genes, 109 genes were up-regulated greater than twofold (e.g., FOSB, THBS1, BHLHB2, EDN1, IL11, FN1, COL10A1, and TUFT1) and 69 genes were down-regulated below 50% (e.g., SMAD6 and DCN). These results suggest that MTA, rather than being a bio-inert material, may have potential to affect the proliferation and differentiation of pulp cells in various ways.

이 연구에서는 mineral trioxide aggregate (MTA)를 in vitro로 인간치수세포에 적용하였을 때 유전자들의 변화를 조사하였다. 실험군은 MTA를 teflon tube (직경 10 mm 길이 2 mm)에 담아 4시간 경화시킨 후HDPCs에 적용하였고, 대조군은 빈tube만을 적용하였다. 6, 24, 72시간 후 total RNA를 추출하여 microarray를 이용하여 분석하여, 2배 이상 또는 절반 이하의 변화를 보이는 유전자 중 선택적으로 역전사 중합효소 연쇄반응(reverse transcriptase polymerase chain reaction)을 사용하여 발현을 확인하였다. 24,546개의 유전자 중에서 109개의 유전자가 2배 이상 up-regulation되었으며(예. FOSB, THBS1, BHLHB2, EDN1,IL11, FN1, COL10A1, TUFT1) 69개의 유전자가 50%이하로 down-regulation되었다(예. SMAD6, DCN). MTA는 bio-inert한 재료라기 보다는 치수세포에 다양한 경로로 영향을 주는 재료로 사료된다. 특히 치수세포의 분화와 증식에 관여하는 유전자의 변화에 영향을 주며 석회화 과정에 관여하는 유전자의 변화에 직접적인 영향을 주리라 사료된다.

Keywords

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