The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Clematidis Radix Extract on Osteoclastogenesis and Gene Expression in RANKL-induced RAW 264.7 Cell

위령선(威靈仙)이 RANKL 처리 RAW 264.7 Cell에 미치는 영향

  • Song, Young-Hun (Dept. of Gynecology, College of Oriental Medicine, Dae-Jeon University) ;
  • Yoo, Jeong-Eun (Dept. of Gynecology, College of Oriental Medicine, Dae-Jeon University) ;
  • Lim, Hyun-Jung (Dept. of Gynecology, College of Oriental Medicine, Dae-Jeon University) ;
  • Yoo, Dong-Youl (Dept. of Gynecology, College of Oriental Medicine, Dae-Jeon University)
  • 송영훈 (대전대학교 한의과대학 부인과교실) ;
  • 유정은 (대전대학교 한의과대학 부인과교실) ;
  • 임현정 (대전대학교 한의과대학 부인과교실) ;
  • 유동열 (대전대학교 한의과대학 부인과교실)
  • Received : 2010.07.29
  • Accepted : 2010.08.10
  • Published : 2010.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: This study was performed to evaluate the effect of Clematidis Radix extract(CB) on osteoclast differentiation and gene expression. The osteocastogenesis and gene expression were determined in RANKL-induced RAW 264.7 cell. Methods: RANKL-induced RAW 264.7 cell with Clematidis Radix extract was stained by TRAP which is expressive marker of osteoclast. The gene expression of RANK, $TNF{\alpha}$, IL-6, iNOS and Cathepsin, those are factors related to bone resorption, was estimated by using Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Results: Clematidis Radix extract decreased the number of TRAP-positive multi nuclei cell, and decreased the gene expression of RANK, $TNF{\alpha}$, IL-6, iNOS and Cathepsin K in RANKL-induced RAW 264.7 cell. Conclusion: It is concluded that Clematidis Radix extract might decrease the bone resorption resulted from decrease of osteoclast differentiation and it's related gene expression.

Keywords

References

  1. 대한내분비학회. 골다공증 진단 및 치료지침 2007. 대한내분비학회지. 2008; 23(2):76.
  2. Canalis E. Mechanisms of Glucocorticoid action in Bone. Implications to glucocorticoid-induced osteoporosis. J Clion Endocri Met. 1996;81:3441-7. https://doi.org/10.1210/jc.81.10.3441
  3. Pietschmann P et al. Osteoporosis, an age-related and gender-specific disease. Gerontology. 2008;55(1):3-12. https://doi.org/10.1159/000166209
  4. Pacifici R. Estrogen deficiency, T cells and bone loss. Cell Immunol. 2008; 252(1-2):68-80. https://doi.org/10.1016/j.cellimm.2007.06.008
  5. Kumamoto H et al. Expression of parathyroid hormone-related protein (PTHrP), osteoclast differentiation factor (ODF)/receptor activator of nuclear factor-kappaB ligand(RANKL) and osteoclastogenesis inhibitory factor(OCIF) /osteoprotegerin(OPG) in ameloblastomas. J. Oral Pathol. Med. 2004;33(1):46-52. https://doi.org/10.1111/j.1600-0714.2004.00204.x
  6. Reddy SV. Regulatory mechanisms operative in osteoclasts. Crit Rev. Eukaryot Gene Expr. 2004;14(4):255-70. https://doi.org/10.1615/CritRevEukaryotGeneExpr.v14.i4.20
  7. Boyce BF et al. Roles for NFkappaB and c-Fos in osteoclasts. J Bone Miner Metab. 2005;23:11-5. https://doi.org/10.1007/BF03026317
  8. Murakami A et al. Phenethyl isothiocyanate suppreases receptor activator of NF-kappaB ligand (RANKL)-induced osteoclastogenesis by blocking activation of ERK1/2 and p38 MAPK in RAW264.7 macrophages. Biofactors. 2007;30(1) :1-11. https://doi.org/10.1002/biof.5520300101
  9. Pang M et al. AP-1 stimulates the cathepsin K promoter in RAW 264.7 cells. Gene. 2007;403(1-2):151-8. https://doi.org/10.1016/j.gene.2007.08.007
  10. Lee ZH et al. Signal transduction by receptor activator of nuclear factor kappa B in osteoclasts. Biochem. Biophys. Res. Commun. 2003;305(2) :211-4. https://doi.org/10.1016/S0006-291X(03)00695-8
  11. 王氷注. 黃帝內經素問. 서울:大成出版社. 1986:21, 101, 212, 274, 340, 768.
  12. 이응세 등. 골다공증의 동의학적 임상문헌에 관한 고찰. 한방재활의학회지. 1997:7(1):437-56.
  13. 藏位壓. 王和鳴編:中國骨病學. 北京:人民衛生出版社1990:249-58.
  14. 陳可培. 中國傳統康復醫學. 北京:人民衛生出版社. 1998:555-8.
  15. 蔡新吉. 腎虛證與骨廣物含量的關係. 山東:山東中醫學院學報. 1992:16(2):52.
  16. 呂犱政. 常見丙最新療法. 北京:中國中醫學出版社. 1994:371-3.
  17. 한방재활의학과학회. 한방재활의학. 군자출판사. 2003:110, 168.
  18. 정재숙. 生血補髓湯이 난소적출 흰쥐 대퇴골의 형태계측학적 변화 및 골대사 관련 인자에 미치는 영향. 世明大學校大學院, 2005.
  19. 金根模. 鹿茸및 六味地黃湯加鹿茸이 白鼠의 運動抑制性골다공증에 미치는 影響, 慶山大學校大學院. 1994.
  20. 朴鍾徹. 左歸飮과 右歸飮이 卵巢摘出白鼠의 性호르몬과 脂質및 骨代謝에 미치는 影響. 大韓韓方婦人科學會誌.1995;8(1):1-27.
  21. 李相坤. 四物湯과 六味地黃湯이 卵巢摘出로 誘導된 白鼠의 골다공증에 미치는 影響. 慶山大學校大學院, 1996.
  22. 曺永任. 借力丸變方이 卵巢摘出로 誘導된 白鼠의 골다공증에 미치는 影響. 大田大學校大學院, 1999.
  23. 전국한의과대학본초학교수. 본초학. 서울:영림사, 2000:307.
  24. 안덕균. 한국본초도감. 서울:교학사.1999:304.
  25. 한방약리학교재편찬위원회. 한방약리학. 서울:신일상사. 2005:201, 202.
  26. 송승원. 威靈仙이 류마토이드 관절염 환자에서 분리한 활액세포의 면역반응에 미치는 영향. 大田大學校大學院.2003.
  27. 유석. 威靈仙이 관절염 유발 동물모델의 소염, 진통 및 해열작용에 미치는 영향. 東國大學校大學院. 2004.
  28. 대한 병리학회. 병리학. 서울:고문사 1991:1183-5.
  29. 徐舞圭. 成人病老人醫學서울:고려의학. 1993:483-6.
  30. 민헌기. 임상 내분비학. 서울:고려의학. 1990:220-2, 415-21, 489-98.
  31. 杜鎬京. 東醫腎系學. 서울:東洋醫學硏究院. 1992:578-84, 1240-53.
  32. 羅元愷. 中醫婦科學. 河北省:人民衛生出版社. 1988:17-22, 161-3.
  33. 陳自明. 婦人良方大全1卷. 文光圖書有限公司. 1987:38.
  34. 葉天士. 葉天士女科. 서울:大星文化社. 1989:212, 213.
  35. 최영길. 내분비학. 의학출판사. 1994:483-90, 573-80.
  36. 김영설. 골다공증의 약물치료. 제44회 대한내과학회 추계학회지. 1992:50-3.
  37. Meiyanto E et al. Osteoclast differentiation factor modulates cell cycle machinery and causes a delay in s phase progression in RAW 264.7 cells. Biochem. Biophys. Res. Commun. 2001:282(1):278-83. https://doi.org/10.1006/bbrc.2001.4564
  38. John T et al. Parathyroid hormone -dependent signaling pathways regulating genes in bone cells, Gene. 2002; 282(1-2):1-17. https://doi.org/10.1016/S0378-1119(01)00798-3
  39. Cho ES et al.. Rolipram, a phosphodiesterase 4 inhibitor, stimulates osteoclast formation by inducing TRANCE expression in mouse calvarial cells. Arch. Pharm. Res. 2004;27(12):1258-62. https://doi.org/10.1007/BF02975891
  40. Khosla S. Minireview. the OPG/ RANKL/RANK system. Endocrinology. 2001;142(12):5050-5. https://doi.org/10.1210/en.142.12.5050
  41. Gallea S et al. Activation of mitogen -activated protein kinase cascades is involved in regulation of bone morphogenetic protein-2-induced osteoblast differentiation in pluripotent C2, C12 cells, Bone. 2001;28(5):491-8. https://doi.org/10.1016/S8756-3282(01)00415-X
  42. Yao Z et al. Tumor necrosis factor -alpha increases circulating osteoclast precursor numbers by promoting their proliferation and differentiation in the bone marrow through upregulation of c-Fos expression, J. Biol. Chem. 2006;281(17):11846-55. https://doi.org/10.1074/jbc.M512624200
  43. Liu XH et al. Interactive effect of interleukin-6 and prostaglandin E2 on osteoclastogenesis via the OPG/ RANKL/RANK system, Ann NY. Acad. Sci. 2006;1068:225-33. https://doi.org/10.1196/annals.1346.047
  44. Lin TH et al. PPAR gamma inhibits osteogenesis via the down-regulation of the expression of COX-2 and iNOS in rats, Bone. 2007;41(4):562-74. https://doi.org/10.1016/j.bone.2007.06.017
  45. Zheng H et al. Collin Osdoby P.. RANKL stimulates inducible nitric -oxide synthetase expression and nitric oxide production in developing osteoclasts. An autocrine negative feedback mechanism triggered by RANKLinduced interferon-beta via NF-kappa B that restrains osteoclastogenesis and bone resorption, J. Biol. Chem. 2006:281(23):15809-20. https://doi.org/10.1074/jbc.M513225200
  46. Matsumoto M et al. Essential role of p38 mitogen-activated protein kinase in cathepsin K gene expression during osteoclastogenesis through association of NFATc1 and PU.1, J. Biol. Chem. 2004:279(44):45969-79.