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http://dx.doi.org/10.15204/jkobgy.2010.23.3.078

Effects of Clematidis Radix Extract on Osteoclastogenesis and Gene Expression in RANKL-induced RAW 264.7 Cell  

Song, Young-Hun (Dept. of Gynecology, College of Oriental Medicine, Dae-Jeon University)
Yoo, Jeong-Eun (Dept. of Gynecology, College of Oriental Medicine, Dae-Jeon University)
Lim, Hyun-Jung (Dept. of Gynecology, College of Oriental Medicine, Dae-Jeon University)
Yoo, Dong-Youl (Dept. of Gynecology, College of Oriental Medicine, Dae-Jeon University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.23, no.3, 2010 , pp. 78-90 More about this Journal
Abstract
Purpose: This study was performed to evaluate the effect of Clematidis Radix extract(CB) on osteoclast differentiation and gene expression. The osteocastogenesis and gene expression were determined in RANKL-induced RAW 264.7 cell. Methods: RANKL-induced RAW 264.7 cell with Clematidis Radix extract was stained by TRAP which is expressive marker of osteoclast. The gene expression of RANK, $TNF{\alpha}$, IL-6, iNOS and Cathepsin, those are factors related to bone resorption, was estimated by using Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Results: Clematidis Radix extract decreased the number of TRAP-positive multi nuclei cell, and decreased the gene expression of RANK, $TNF{\alpha}$, IL-6, iNOS and Cathepsin K in RANKL-induced RAW 264.7 cell. Conclusion: It is concluded that Clematidis Radix extract might decrease the bone resorption resulted from decrease of osteoclast differentiation and it's related gene expression.
Keywords
RANKL-induced RAW 264.7 Cell; Clematidis Radix; Osteoporosis;
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Times Cited By KSCI : 2  (Citation Analysis)
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