The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Boyanghwano-Tang on the Development of Experimentally-induced Endometriosis in the Rats

보양환오탕(補陽還五湯)이 실험적으로 유발된 흰쥐의 자궁내막증에 미치는 영향

  • Lim, Sung-Min (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Lee, Dong-Nyung (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Seo, Il-Bock (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University) ;
  • Kim, Hyung-Jun (Dept. of Oriental Gynecology, College of Oriental Medicine, Se-Myung University)
  • 임성민 (세명대학교 한의과대학 한방부인과교실) ;
  • 이동녕 (세명대학교 한의과대학 한방부인과교실) ;
  • 서일복 (세명대학교 한의과대학 한방부인과교실) ;
  • 김형준 (세명대학교 한의과대학 한방부인과교실)
  • Received : 2010.07.28
  • Accepted : 2010.08.10
  • Published : 2010.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: This study was performed to investigate effects of Boyanghwano-Tang on the development of experimentally-induced endometriosis in rats. Methods: Endometriosis was induced in rats by autotransplanting uterine tissue to the peritoneum and divided them into three groups: (1) sham-operated group(n=8), (2) surgically induced endometriosis and untreated control group (n=8), (3) surgically induced endometriosis and Boyanghwano-Tang treated group. Boyanghwano-Tang was orally administrated for 15 days after operation. Then we measured the body weight, the volume of endometriotic implants, the weight of uterus and ovary, and investigated the concentration of cytokines (MCP-1, TNF-$\alpha$, IL-$1{\beta}$, IL-6) in peritoneal fluids. Histopathology, immunohistochemistry for COX-2 and VEGF, and histochemistry for mast cell in transplanted uterine tissue were performed. Results: - The volume($mm^3$) of endometriotic implants in Boyanghwano-Tang treated group ($122.3{\pm}45.0$) was significantly decreased(p<0.05) compared with control group($222.1{\pm}109.1$). - The concentration(pg/$m{\ell}$) of MCP-1 in peritoneal fluids in Boyanghwano-Tang treated group($1026.3{\pm}196.5$) was significantly decreased(p<0.05) compared with control group($1412.5{\pm}345.7$). - The concentration(pg/$m{\ell}$) of TNF-$\alpha$ in peritoneal fluids in Boyanghwano-Tang treated group($936.5{\pm}94.3$) was significantly decreased(p<0.01) compared with control group($1126.2{\pm}139.9$). - The concentration(pg/$m{\ell}$) of IL-$1{\beta}$ in peritoneal fluids in Boyanghwano-Tang treated group($78.5{\pm}13.3$) was significantly decreased(p<0.01) compared with control group($105.3{\pm}17.6$). - The concentration(pg/$m{\ell}$) of IL-6 in peritoneal fluids in Boyanghwano-Tang treated group($107.4{\pm}15.8$) was decreased compared with control group($122.8{\pm}19.3$). - Histopathologically, proliferation of endometriotic epithelia, infiltration of inflammatory cells and angiogenesis in transplanted uterine tissue of Boyanghwano-Tang treated group were weakly observed than those of control group. - The percentage of positive epithelial layers for COX-2 in Boyanghwano-Tang treated group($51.5{\pm}14.1$) was significantly decreased(p<0.01) compared with control group($75.1{\pm}16.3$). - The VEGF expression of endometriotic epithelia, neovascular endothelia and stromal cells in transplanted uterine tissue of Boyanghwano-Tang treated group were weakly observed than those of control group. - The number of mast cells in adjacent tissue of transplanted uterine tissue in Boyanghwano-Tang treated group($75.9{\pm}13.1$) was decreased compared with control group($91.0{\pm}28.3$). Conclusion: On the basis of these results, we concluded that Boyanghwano-Tang have inhibiting effects on the development of transplanted uterine tissue. And these effects may be related with decreased production of MCP-1, TNF-$\alpha$, IL-$1{\beta}$ and decreased expression of COX-2 and VEGF by administration of Boyanghwano-Tang.

Keywords

References

  1. Black AY, Jamieson MA. Adolescent endometriosis. Current opinion in obstetrics and gynecology. 2002;14 :467-74. https://doi.org/10.1097/00001703-200210000-00005
  2. 강길전. 여성생식의학. 서울:대관출판사. 1998:413-27.
  3. 서울대학교 의과대학 산부인과교실. 산부인과학. 서울:군자출판사. 1999:602-8.
  4. Kwok A, Lam A, Ford R. Deeply infiltrating endometriosis: implication, diagnosis and management. Obstetrical and gynecological survey. 2001;56(3):168-77. https://doi.org/10.1097/00006254-200103000-00024
  5. Solnik MJ. Chronic pelvic pain and endometriosis in adolescents. Current opinion in obstetrics and gynecology. 2006;18:511-8. https://doi.org/10.1097/01.gco.0000242953.94749.2b
  6. Martin DC, Ling FW. Endometriosis and pain. Clinical obstetrics and gynecology. 1999;42(3):664-86. https://doi.org/10.1097/00003081-199909000-00019
  7. Kim AH, Adamson GD. Surgical treatment options for endometriosis. Clinical obstetrics and gynecology. 1999;42(3):633-44. https://doi.org/10.1097/00003081-199909000-00017
  8. Witz CA. Current concepts in the pathogenesis of endometriosis. Clinical obstetrics and gynecology. 1999;42(3) :566-85. https://doi.org/10.1097/00003081-199909000-00013
  9. Ryan IP, Taylor RN. Endometriosis and infertility: new concepts. Obstetrical and gynecological survey. 1999;54(11) :197-203. https://doi.org/10.1097/00006254-199911001-00030
  10. Marsh EE, Laufer MR. Endometriosis in premenarcheal girls who do not have an associated obstructive anomaly. Fertil Steril. 2005;83:758-60. https://doi.org/10.1016/j.fertnstert.2004.08.025
  11. 이태균. 子宮內膜症에 대한 文獻的考察. 大韓韓方婦人科學會誌. 1995;8(1) :161-74.
  12. 韓醫婦人科學敎材編纂委員會. 韓醫婦人科學上卷. 서울:정담. 2002:175, 186-90.
  13. 윤영희 등. 少腹逐瘀湯이 실험적으로 유발된 흰쥐의 자궁내막증에 미치는 영향. 大韓韓方婦人科學會誌. 2006;19(2) :141-61.
  14. 김철수 등. 膈下逐瘀湯이 실험적으로 유발된 흰쥐의 자궁내막증에 미치는 영향. 大韓韓方婦人科學會誌. 2007;20(1):61-83.
  15. 장성환, 임은미, 이은. 桃紅四物湯이 자궁내막증을 유발한 흰쥐에 미치는 영향. 大韓韓方婦人科學會誌. 2006;19(1):125-38.
  16. 김여태 등. 加味歸朮破癥湯이 실험적으로 유발된 흰쥐의 자궁내막증에 미치는 영향. 大韓韓方婦人科學會誌. 2006;19(2):162-85.
  17. 이지연, 임은미, 권기록. 加味紅藤湯 직장내 투여가 자궁내막증을 유발한 흰쥐에 미치는 영향. 大韓韓方婦人科學會誌. 2006;19(3):109-20.
  18. 이미정, 임은미, 권기록. 八珍湯이 자궁내막증을 유발한 흰쥐에 미치는 영향. 大韓韓方婦人科學會誌. 2006;19(3):83-94.
  19. 김성혜, 권기록, 박희수. 산삼 약침이 자궁내막증 유발 흰쥐에 미치는 영향. 大韓鍼灸學會誌. 2006;23(5):55-67.
  20. 조현주, 김윤상, 임은미. 黃芪가 자궁 내막증을 유발 흰쥐에 미치는 영향. 大韓韓方婦人科學會誌. 2007;20(2):43-59.
  21. 황우준. (國譯)醫林改錯. 익산:圓光大學校出版局. 1998:126, 27.
  22. 윤용갑. 東醫方劑와 處方解說. 서울: 醫聖堂. 2007:690, 91.
  23. 김동웅. 졸중풍 후유증에 의한 운동장애 환자의 치료를 위한 통용방으로서 萬金湯과 補陽還五湯의 효과에 관한 비교 연구. 大韓韓醫學會誌. 1994 ;15(2):233-40.
  24. 문성수, 이상용. 加減補陽還五湯이 생쥐의 학습과 기억의 감퇴 및 cetylcholinesterase의 억제에 미치는 영향. 동의신경정신과학회지. 2000;11(1) :19-36.
  25. 정용준, 김동웅. 뇌혈관질환에 의한 편마비 환자에서 補陽還五湯의 효과. 大韓東醫病理學會誌. 1999;13(2):54-8.
  26. 이윤재 등. 산화적 손상에 의해 유발된 심근세포 독성에 대한 補陽還五湯의 방어 효과. 大韓韓方內科學會誌.2005;26(2):409-19.
  27. 이진우, 정우열. 活血化瘀藥物을 응용한 새로운 암 치료제 개발 연구. 大韓東醫病理學會誌. 2000;14(1):45-50.
  28. 하종천 등. 補陽還五湯에 의한 비특이적 세포독성 T세포 활성 증강. 생약학회지. 2001;32(3):226-32.
  29. 김영현. 항어혈 치료제인 補陽還五湯의 면역 조절 작용. 전주대학교대학원. 2002.
  30. Vernon MW, Wilson EA. Studies on the surgical induction of endometriosis in the rat. Fertil Steril. 1985;44(5) :684-94. https://doi.org/10.1016/S0015-0282(16)48988-0
  31. 대한산부인과학회. 부인과학 제3판. 서울:칼빈서적. 1997:566-97.
  32. Winkel CA. Combined medical and surgical treatment of women with endometriosis. Clinical obstetrics and gynecology. 1999;42(3):645-63. https://doi.org/10.1097/00003081-199909000-00018
  33. Buyalos RP, Agarwal SK. Endometriosis -associated infertility. Current opinion in obstetrics and gynecology. 2000;12 :377-81. https://doi.org/10.1097/00001703-200010000-00006
  34. Burns WN, Schenken RS. Pathophysiology of Endometriosis associated infertility. Clinical obstetrics and gynecology. 1999;42(3):586-610. https://doi.org/10.1097/00003081-199909000-00014
  35. Honore GM. Extrapelvic endometriosis. Clinical obstetrics and gynecology. 1999;42(3):699-711. https://doi.org/10.1097/00003081-199909000-00021
  36. 이경호 등. 자궁내막증 환자에서 microlaparoscopy를 이용한 pain mapping 의 유용성. 대한산부회지. 2000;43(12) :2166.
  37. 이정호 등. 자궁내막증의 임상적 고찰. 대한산부회지. 1990;33(6):770-5.
  38. 김동호, 이재찬, 배도환. 진단 및 수술적 골반경하에서 자궁내막증의 빈도와 임상적 고찰. 대한산부회지. 1996;39(11):2093.
  39. 임용택. 자궁내막증 처치의 최신 지견. 대한산부회지. 2005;48(3):535
  40. Reddy S, Rock JA. Treatment of endometriosis. Clinical obstetrics and gynecology. 1998;41(2):387-92. https://doi.org/10.1097/00003081-199806000-00020
  41. Ferrero et al. Future perspectives in the medical treatment of endometriosis. Obstetrical and gynecological survey. 2005;60(12):817-26. https://doi.org/10.1097/01.ogx.0000189153.87365.dc
  42. Revelli A et al. Recurrent endometriosis: a review of biological and clinical aspects. Obstetrical and gynecological survey. 1995;50(10):747-54. https://doi.org/10.1097/00006254-199510000-00022
  43. 최승훈. 韓方病理學. 서울:일중사. 1997;161-74.
  44. 채우석. 면역 질환의 韓方槪念과 治療에 관한 文獻的考察. 大韓韓醫學會誌. 1990;11(2):54-91.
  45. 김동일, 이동규, 이태균. 자궁내막증의 韓醫學治療에 관한 연구. 大韓韓方婦人科學會誌. 2002;15(2):126-43.
  46. 郭陳烈, 金宰源. 醫林改錯評譯. 서울:成輔社. 1998:166-74.
  47. 韓醫科大學方劑學敎授. 方劑學. 서울: 永林社. 1999:414-6.
  48. 이귀녕, 권오헌. 임상병리파일. 서울:의학문화사. 2000:717-31.
  49. Barcz E, Kaminski P, Marianowski L. Role of cytokines in pathogenesis of endometriosis. Medical science monitor. 2000;6(5):1042-6.
  50. Jolicoeur C et al. Incrased expression of monocyte chemotactic protein-1 in the endometrium of women with endometriosis. American journal of pathology. 1998;152(1):125-33.
  51. Zeyneloglu HB et al. The role of monocyte chemotactic protein-1 in intraperitoneal adhesion formation. Human reproduction. 1998;13(5):1194-9. https://doi.org/10.1093/humrep/13.5.1194
  52. Bedaiwy MA et al. Prediction of endometriosis with serum and peritoneal fluid markers: a prospective controlled trial. Human reproduction. 2002;17(2) :426-31. https://doi.org/10.1093/humrep/17.2.426
  53. Shakiba K, Falcone T. Tumor necrosis factor-$\alpha$ blockers: potential limitations in the manegement of endometriosis. Human reproduction. 2006;21(9):2417-20. https://doi.org/10.1093/humrep/del179
  54. Iwabe T et al. Tumor necrosis factor-$\alpha$ promote proliferation of endometriotic stromal cells by inducing interleukin-8 gene and protein expression. The journal of endocrinology and metabolism. 2000 ;85(2):824-9. https://doi.org/10.1210/jc.85.2.824
  55. Cheong YC et al. IL-1, IL-6 and TNF-$\alpha$ concentrations in the peritoneal fluid in women with pevic adhesions. Human reproduction. 2002;17(1):69-75. https://doi.org/10.1093/humrep/17.1.69
  56. Levobic DI et al. Induction of an angiogenic phenotype in endometriotic stromal cell cultures by interleukin -$1\beta$. Molecular human production. 2000;6(3):269-75. https://doi.org/10.1093/molehr/6.3.269
  57. Meresman GF et al. Effect of GnRH analogues on apoptosis and release of interleukin-$1\beta$ and Vascular endothelial growth factor in endometrial cell cultures from patients with endometriosis. Human reproduction. 2003;18(9):1767-71. https://doi.org/10.1093/humrep/deg356
  58. Yoshida S et al. A combination of interleukin-6 and its soluble receptor impairs sperm motility: implications in infertility associated with endometriosis. Human reproduction. 2004;19(8):1821-5. https://doi.org/10.1093/humrep/deh324
  59. Somigliana E et al. Use of the concomitant serum dosage of CA 125, CA 19-9 and interleukin-6 to detect the presence of endometriosis. Human reproduction. 2004;19(8):1871-6. https://doi.org/10.1093/humrep/deh312
  60. Ota H, Igarashi S, Sasaki M, Tanaka T. Distribution of cyclooxygenase-2 in eutopic and ectopic endometrium in endometriosis and adenomyosis. Human reproduction. 2001;16(3):561-6. https://doi.org/10.1093/humrep/16.3.561
  61. Fagotti A et al. Analysis of cyclooxygenase-2(COX-2) expression in different sites of endometriosis and correlation with clinico-pathological parameters. Human reproduction. 2004;19(2):393-7. https://doi.org/10.1093/humrep/deh054
  62. Wu MH et al. Distinct mechanisms regulate cyclooxygenase-1 and -2 in peritoneal macrophages in women with and without endometriosis. Molecular human reproduction. 2002;8(12):1103-10. https://doi.org/10.1093/molehr/8.12.1103
  63. Mclaren J et al. Vascular endothelial growth factor(VEGF) concentrations are elevated in peritoneal fluid of women with endometriosis. Obstetrical and gynecological survey. 1996;51(8) :488-90. https://doi.org/10.1097/00006254-199608000-00020
  64. Mclaren J et al. Vascular endothelial growth factor is produced by peritoneal fluid macrophages in endometriosis and is regulated by ovarian steroids. The journal of clinical investigation. 1996;98(2):482-9. https://doi.org/10.1172/JCI118815
  65. Nguyen M, Pace AJ, Koller BH. Age-induced reprogramming of mast cell degranulation. The journal of immunology. 2005;175:5701-7. https://doi.org/10.4049/jimmunol.175.9.5701
  66. Sugamata M, Ihara T, Uchiide I. Increase of activated mast cells in human endometriosis. American journal of reproductive immunology. 2005; 53(3):120-5. https://doi.org/10.1111/j.1600-0897.2005.00254.x
  67. Xu X et al. Roles of mast cells and myofibloblasts in human peritoneal adhesion formation. Annals of surgery. 2002;236(5):593-601. https://doi.org/10.1097/00000658-200211000-00009