The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Anti-oxidant Effects of Samultang-Gami on MEF Cells

사물탕가미방(四物湯加味方)의 항산화 활성에 대한 실험적 연구

  • Jung, Jae-Joong (Dept. of OB & GY, Graduate school of Oriental Medicine, Dong-Guk Univ.) ;
  • Goo, Sun-Young (Dept. of Life Science, Dong-Guk Univ.) ;
  • Go, Eun-Bi (Dept. of Life Science, Dong-Guk Univ.) ;
  • Sung, Jung-Suk (Dept. of Life Science, Dong-Guk Univ.) ;
  • Kim, Dong-Il (Dept. of OB & GY, Graduate school of Oriental Medicine, Dong-Guk Univ.)
  • 정재중 (동국대학교 한의과대학 한방부인과교실) ;
  • 구선영 (동국대학교 바이오시스템대학 생명과학과) ;
  • 고은비 (동국대학교 바이오시스템대학 생명과학과) ;
  • 성정석 (동국대학교 바이오시스템대학 생명과학과) ;
  • 김동일 (동국대학교 한의과대학 한방부인과교실)
  • Received : 2010.07.28
  • Accepted : 2010.08.10
  • Published : 2010.08.31
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Abstract

Purpose: This experiment is designed to find out anti-oxidant effects of Samultang-Gami which was composed of Rehmanniae Radix(RR), Angelicae Gigantis Radix, Cnidii Rhizoma(CR), Paeoniae Radix(PR), Cortex Moutan Radicis, Hedyotis Diffusa(HD) and Caesalpinia Sappan on MEF cells. Methods: In vitro antioxidant effects were measured by MTT assay, DPPH assay, cell cycle analysis, AnnexinV-FITC/PI assay and DAPI staining using MEF cells treated with various concentrations of 70% ethanol extract of Samultang-Gami. Results: 1. In the scavenging for DPPH radical, the each treated groups of PR, CR and HD showed positive effects. RR and CR increased the viability of oxidative damaged MEF cells in a dose-dependent manner. 2. 70% ethanol extract of Samultang-Gami was shown best antioxidative effect in the concentration of $0.5mg/m{\ell}$. 3. The treatment of Samultang-Gami in oxidative damaged MEF cells didn't have any effect on cell cycle restoration. but it could lower late apoptosis rate a little and be observed the protection of nucleus. Conclusion: It can be concluded that Samultang-Gami, RR and CR have antioxidant effects on MEF cells.

Keywords

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