The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Inhibitory effects of Nardostachys Jatamansi on the maturation of dendritic cells

감송향이 수지상세포 성숙에 미치는 영향

  • O, Kwang-Woo (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University) ;
  • Jeong, Ji-Hye (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University) ;
  • Cheong, Hyun-Cheol (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University) ;
  • Cho, Han-Baek (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University) ;
  • Kim, Song-Baeg (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University) ;
  • Choe, Chang-Min (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University)
  • 오광우 (원광대학교 한의과대학 부인과학교실) ;
  • 정지혜 (원광대학교 한의과대학 부인과학교실) ;
  • 정현철 (원광대학교 한의과대학 부인과학교실) ;
  • 조한백 (원광대학교 한의과대학 부인과학교실) ;
  • 김송백 (원광대학교 한의과대학 부인과학교실) ;
  • 최창민 (원광대학교 한의과대학 부인과학교실)
  • Received : 2010.07.29
  • Accepted : 2010.08.10
  • Published : 2010.08.31
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Abstract

Purpose: The purpose of this study is to investigate inhibitory effect on the maturation of dendritic cells from aqueous extract from Nardostachys Jatamansi(NJ). Methods: I examined the phenotypic maturation(class II MHC, CD40, CD86), expression of pro-inflammatory cytokine(TNF-$\alpha$, IL-6, IL-12) and endocytosis of FITC-Dextran in the LPS-induced bone marrow-derived dendritic cells(BMDCs) of mice. Furthermore, the Western-blot analysis reveals the mechanism of inhibitory effect. Results: 1. The NJ extract inhibited the phenotypic maturation of BMDCs in a dose-dependent manner. 2. The NJ extract inhibited the LPS induced cytokine production of BMDCs in a dose-dependent manner. 3. The NJ extract enhanced the endocytosis of Dex-FITC in LPS treated DC. 4. The NJ extract inhibited the activation of JNK and p38 phosphorylation, but not ERK phosphorylation of MAPK family and doesn't inhibit Ik-Ba degradation in LPS-stimulated BMDCs. Conclusion: These results suggest that NJ extract is able to attenuate the inflammation and maturation in BMDCs and may inhibit proliferation of T cells. In conclusion, this experiment suggests that NJ extract may be useful in hypersensitivity disease including autoimmune disease.

Keywords

References

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