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http://dx.doi.org/10.15204/jkobgy.2010.23.3.026

Anti-oxidant Effects of Samultang-Gami on MEF Cells  

Jung, Jae-Joong (Dept. of OB & GY, Graduate school of Oriental Medicine, Dong-Guk Univ.)
Goo, Sun-Young (Dept. of Life Science, Dong-Guk Univ.)
Go, Eun-Bi (Dept. of Life Science, Dong-Guk Univ.)
Sung, Jung-Suk (Dept. of Life Science, Dong-Guk Univ.)
Kim, Dong-Il (Dept. of OB & GY, Graduate school of Oriental Medicine, Dong-Guk Univ.)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.23, no.3, 2010 , pp. 26-37 More about this Journal
Abstract
Purpose: This experiment is designed to find out anti-oxidant effects of Samultang-Gami which was composed of Rehmanniae Radix(RR), Angelicae Gigantis Radix, Cnidii Rhizoma(CR), Paeoniae Radix(PR), Cortex Moutan Radicis, Hedyotis Diffusa(HD) and Caesalpinia Sappan on MEF cells. Methods: In vitro antioxidant effects were measured by MTT assay, DPPH assay, cell cycle analysis, AnnexinV-FITC/PI assay and DAPI staining using MEF cells treated with various concentrations of 70% ethanol extract of Samultang-Gami. Results: 1. In the scavenging for DPPH radical, the each treated groups of PR, CR and HD showed positive effects. RR and CR increased the viability of oxidative damaged MEF cells in a dose-dependent manner. 2. 70% ethanol extract of Samultang-Gami was shown best antioxidative effect in the concentration of $0.5mg/m{\ell}$. 3. The treatment of Samultang-Gami in oxidative damaged MEF cells didn't have any effect on cell cycle restoration. but it could lower late apoptosis rate a little and be observed the protection of nucleus. Conclusion: It can be concluded that Samultang-Gami, RR and CR have antioxidant effects on MEF cells.
Keywords
Samultang-Gami; MEF cells; Anti-oxidant;
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