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http://dx.doi.org/10.15204/jkobgy.2010.23.3.014

Inhibitory effects of Nardostachys Jatamansi on the maturation of dendritic cells  

O, Kwang-Woo (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University)
Jeong, Ji-Hye (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University)
Cheong, Hyun-Cheol (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University)
Cho, Han-Baek (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University)
Kim, Song-Baeg (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University)
Choe, Chang-Min (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Won-Kwang University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.23, no.3, 2010 , pp. 14-25 More about this Journal
Abstract
Purpose: The purpose of this study is to investigate inhibitory effect on the maturation of dendritic cells from aqueous extract from Nardostachys Jatamansi(NJ). Methods: I examined the phenotypic maturation(class II MHC, CD40, CD86), expression of pro-inflammatory cytokine(TNF-$\alpha$, IL-6, IL-12) and endocytosis of FITC-Dextran in the LPS-induced bone marrow-derived dendritic cells(BMDCs) of mice. Furthermore, the Western-blot analysis reveals the mechanism of inhibitory effect. Results: 1. The NJ extract inhibited the phenotypic maturation of BMDCs in a dose-dependent manner. 2. The NJ extract inhibited the LPS induced cytokine production of BMDCs in a dose-dependent manner. 3. The NJ extract enhanced the endocytosis of Dex-FITC in LPS treated DC. 4. The NJ extract inhibited the activation of JNK and p38 phosphorylation, but not ERK phosphorylation of MAPK family and doesn't inhibit Ik-Ba degradation in LPS-stimulated BMDCs. Conclusion: These results suggest that NJ extract is able to attenuate the inflammation and maturation in BMDCs and may inhibit proliferation of T cells. In conclusion, this experiment suggests that NJ extract may be useful in hypersensitivity disease including autoimmune disease.
Keywords
Nardostachys Jatamansi(NJ); Dendritic cells; Maturation;
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