Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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A Study on the Sensory Characteristic of Yogurt and Antimicrobial Activity of Lactobacillus plantarum LHC52 Isolated from Kimchi

김치에서 분리한 Lactobacillus plantarum LHC52의 항균활성과 요구르트의 관능성 연구

  • Lee, Seung-Gyu (Division of Quality Control and Utilization of Animal Products, National Institute of Animal Science, RDA) ;
  • Han, Ki-Sung (Division of Quality Control and Utilization of Animal Products, National Institute of Animal Science, RDA) ;
  • Jeong, Seok-Geun (Division of Quality Control and Utilization of Animal Products, National Institute of Animal Science, RDA) ;
  • Oh, Mi-Hwa (Division of Quality Control and Utilization of Animal Products, National Institute of Animal Science, RDA) ;
  • Jang, Ae-Ra (Division of Quality Control and Utilization of Animal Products, National Institute of Animal Science, RDA) ;
  • Kim, Dong-Hun (Division of Quality Control and Utilization of Animal Products, National Institute of Animal Science, RDA) ;
  • Bae, In-Hyu (Department of Animal Science and Technology, Sunchun University) ;
  • Ham, Jun-Sang (Division of Quality Control and Utilization of Animal Products, National Institute of Animal Science, RDA)
  • 이승규 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 한기성 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 정석근 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 오미화 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 장애라 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 김동훈 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 배인휴 (순천대학교 동물자원과학과) ;
  • 함준상 (농촌진흥청 국립축산과학원 축산물이용과)
  • Received : 2009.11.20
  • Accepted : 2010.04.13
  • Published : 2010.04.30
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Abstract

The aim of our study was to develop a new starter culture for fermented milk. Polymerase chain reaction screening of 103 acid-producing isolates from Kimchi identified 72 Lactobacillus strains. The ability of the strains to inhibit the growth of the food-borne human pathogens (Escherichia coli, Salmonella Enteritidis, Staphylococcus aureus) was measured, using a conventional paper disk method. Among the 72 strains, strain LHC52 displayed potent antagonistic activity. Use of 16S rDNA sequencing and the API 50CHL system identified the strain as Lactobacillus plantarum and it was designated L. plantarum LHC52. Biochemical analyses revealed especially high antibacterial activity against E. coli. Yogurt produced using L. plantarum LHC52 did not show different microbiological and physicochemical properties compared to conventionally-prepared yogurt, implicating L. plantarum LHC52 as a useful, potently antibacterial starter culture for yogurt preparation.

이 연구는 김치에서 분리한 항균활성이 우수한 요구르트 제조용 유산균 스타터를 개발하기 위함이다. 분리한 103개의 산생성 균주를 PCR로 screening하여 72개의 유산균을 분리하였다. 분리균의 배양액을 paper disk method를 사용하여 병원성 미생물(E. coli, S. Enteritidis, S. aureus)에 대한 항균활성을 측정하였고, 활성이 강한 균주를 선별하여 API 50CHL과 16S rRNA sequencing 방법으로 균을 동정하였다. 균은 L. plantarum으로 확인되어 L.plantarum LHC52로 명명하였다. L. plantarum LHC52는 특히 E. coli에 대해 높은 항균성을 나타내었다. L.plantarum LHC52를 사용하여 제조한 요구르트의 미생물학적, 이화학적 특성과 관능검사 결과 대조구와의 유의적인 차이는 없었다. 그 결과 김치에서 분리한 L. plantarum LHC52의 항균활성이 우수한 요구르트 제조 스타터 균주로서 사용 가능함을 확인하였다.

Keywords

References

  1. DeDios, Pozo-Olano, J., Warran, J. H., Gomez, G. G., and Cavazos, M. G. (1978) Effect of a lactobacilli preparation on traveler's diarrhea. Gastroenterol. 74, 829-839.
  2. Deurenberg, R. H., Vink, C., Kalenic, S., Friedrich, A. W., Bruggeman, C. A., and Stobberingh, E. E. (2007) The molecular evolution of methicillin-resistant Staphylococcus aureus. Clin. Microbiol. Infect. 13, 222-235. https://doi.org/10.1111/j.1469-0691.2006.01573.x
  3. Francis, A. W. (1995) Staphylococcus aureus (including toxic shock syndrom). pp. 1754-1755, Mandell Douglas and Bennetts Principles and Infections Disease. 4th eds., Churchill Livingstone.
  4. Han, S. J., Jung, P. M., Kim, H. G. Hwang, E. H., and Seong, I. W. (1999) Multiple intestinal ulcerations and perforations secondary to methicillin-resistant Staphylococcus aureus enteritis in infants. J. Pediatr. Surg. 34, 381-386. https://doi.org/10.1016/S0022-3468(99)90481-5
  5. Hong, K. H., Nam, E. S., and Park, S. I. (2003) Effect of Omija (Schizandra chinensis) extract on the growth inhibition of food borne pathogens in yoghurt. Kor. J. Food. Sci. Ani. Resour. 23, 342-349.
  6. Jeon, S. R., Song, T. S., Kim, J. Y., Shin, W. C., Her, S. U., and Yoon, S. S. (2007) Identification and characterization of lactic acid bacteria starters isolated from the commercial drink-yogurt products. Kor. J. Food Sci. Ani. Resour. 27, 509-516. https://doi.org/10.5851/kosfa.2007.27.4.509
  7. Jeong, H. J., Kim, W. J., Kim, M. J., and Park, S. C. (1995) Nosocomial infection surveillance in the care unit. Kor. J. Infect. Dis. 27, 105-117.
  8. Kim, S. I., Kim, I. C., and Chang, H. C. (1999) Isolation and identification of antimicrobial agent producing microorganisms and sensitive strain from soil. J. Kor. Soc. Food Sci. Nutr. 28, 526-533.
  9. Klaenhammer, T. R. (1988) Bacteriocins of lactic acid bacteria. Bichimie. 70, 337-349. https://doi.org/10.1016/0300-9084(88)90206-4
  10. Ko, Y. D., Kim, H. J., Chun, S. S., and Sung, N. K. (1994) Development of control system for Kimchi fermentation and storage using refrigerator. Kor. J. Food Sci. Technol. 26, 199-203.
  11. Lee, I. S., Park, W. S., Koo, Y. J., and Kang, K. H. (1994) Changes in some characteristics of brined chinese cabbage of fall cultivars during storage. Kor. J. Food Sci. Technol. 26, 239-245.
  12. Lee, S. G., Lee, Y. J., Kim, M. K., Han, K. S., Jeong, S. G., Jang, A., Chae, H. S., Kim, D. H., and Ham, J. S. (2009) Quality characteristics and inhibitory activity against Staphylococcus aureus KCCM 40510 of Yogurts manufactured with garlic juice. Kor. J. Food Sci. Ani. Resour. 29, 500-505. https://doi.org/10.5851/kosfa.2009.29.4.500
  13. Lim, S. M. and Im, D. S. (2007) Bactericidal effect of bacteriocin of Lactobacillus plantarum K11 isolated from Dongchimi on Escherichia coli O157. J. Food Hyg. Saf. 22, 151-158.
  14. Lim, S. M. and Im, D. S. (2009) Screening and characterization of probiotic lactic acid bacteria isolated from Korean fermented foods. J. Microbiol. Biotechnol. 19, 178-186. https://doi.org/10.4014/jmb.0804.269
  15. Mheen, T. I. and Kwon, T. W. (1984) Effect of temperature and salt concentration on Kimchi fermentation. Kor. J. Food Sci. Technol. 16, 443-450.
  16. Muller, D. M., Carrasco, M. S., Tonarelli, G. G., and Simonetta, A. C. (2009) Characterization and purification of a new bacteriocin with a broad inhibitory spectrum produced by Lactobacillus plantarum lp31 strain isolated from dry-fermented sausage. J. Appl. Microbiol. 106, 2031-2040. https://doi.org/10.1111/j.1365-2672.2009.04173.x
  17. Nguyen, T. D., Kang, J. H., and Lee, M. S. (2007) Characterization of Lactobacillus plantarum PH04, a potential probiotic bacterium with cholesterol-lowering effects. 2007. Int. J. Food Microbiol. 113, 358-361. https://doi.org/10.1016/j.ijfoodmicro.2006.08.015
  18. Park, S. G., Hwang, Y. O., Jung, J. H., and Lee, K. M. (2001) Biological characteristics of Staphylococcus aureus isolated from food-borne patients in Seoul. J. Food. Hyg. Saf. 16, 159-167.
  19. Park, W. S., Lee, I. S., Han, Y. S., and Koo, Y. J. (1994) Kimchi preparation with brined Chinese cabbage and seasoning mixture stored separately. Kor. J. Food Sci. Technol. 26, 231-238.
  20. Rhee, Y. H. and Kang, M. S. (1996) Characteristics of betagalactosidase activity in Lactobacillus plantarum from Kimchi. Agr. Chem. Biotechnol. 39, 60-66.
  21. Rhee, Y. H. and Kang, M. S. (1996) Physico-chemical characteristics and -$\beta$galactosidase activity of Lactobacillus plantarum from Kimchi. Agr. Chem. Biotechnol. 39, 54-59.
  22. Sanders, M. E. and Huis in't Veld, J. (1999) Bringing a probiotic- containing functional food to the market:microbiological, product, regulatory and labeling issues. Antonie Van Leeuwenhoek. 76, 293-315. https://doi.org/10.1023/A:1002029204834
  23. So, M. H. and Kim, Y. B. (1995) Cultural characteristics psychrotrophic lactic acid bacteria isolated from Kimchi. Kor. J. Food Sci. Technol. 27, 506-515.
  24. Straume, D., Johansen, R. F., Bjoras, M., Nes, I. F., and Diep, D. B. (2009) DNA binding kinetics of two response regulators, PlnC and PlnD, from the bacteriocin regulon of Lactobacillus plantarum C11. BMC. Biochem. 10, 17-27. https://doi.org/10.1186/1471-2091-10-17
  25. Taylor, J., Hirsch, A., and Mattick, A. R. T. (1949) The treatment of bovine streptococcal and staphylococcal mastitis with nisin. Vet. Rec. 61, 197-198
  26. Tsai, T. y., Chu, L. H., Lee, C. L., and Pan, T. M. (2009) Atherosclerosis- preventing activity of lactic acid bacteria-fermneted milk-soymilk supplemented with Momordica charantia. J. Agr. Food Chem. 57, 2065-2071. https://doi.org/10.1021/jf802936c
  27. Wang, Y., Xu, N., Xi, A., Ahmed, Z., Zhang, B., and Bai, X. (2009) Effects of Lactobacillus plantarum MA2 isolated from Tibet kefir on lipid metabolism and intestinal microflora of rats fed on high-cholesterol diet. Appl. Microbiol. Biotechnol. 84, 341-347. https://doi.org/10.1007/s00253-009-2012-x
  28. Yang, E. J. and Chang, H. C. (2007) Characterization of bacteriocin- like substances produced by Bacillus subtilis MJP1. Kor. J. Microbiol. Biotechnol. 35, 339-346.
  29. Yoon, J. H., Lee, S. T., and Park, Y. H. (1996) Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int. J. Syst. Bacteriol. 48, 187-194.

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