Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지

Evaluation of Antibacterial Effects of a Combination of Coptidis Rhizoma, Lonicerae Flos, Paeonia Japonica Extracts, and Dioctahedral Smectite Against Salmonella Typhimurium in Murine Salmonellosis

  • Jung, Won-Chul (Research Institute of Live Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Yeon-Ok (Senong Co., Ltd.) ;
  • Cha, Chun-Nam (Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University) ;
  • Lee, Yeo-Eun (Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University) ;
  • Kim, Gon-Sup (Research Institute of Live Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Research Institute of Live Sciences, College of Veterinary Medicine, Gyeongsang National University)
  • Received : 2010.01.02
  • Accepted : 2010.01.27
  • Published : 2010.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The present study was undertaken to estimate the antibacterial effect of a combination of C. rhizoma, L. Flos, and P japonica (1:1:1) extracts (CLP1000) and a combination of the herbal extract mixture and dioctahedral smectite (CLPS1000) against murine salmonellosis. At the concentration of CLP1000 and CLPS1000 0.5 mg/ml, the antibacterial effect was not showed on Salmonella typhimurium (S. typhimurium). On the other hand, the antibacterial effect against S. typhimurium was observed at the concentration of CLP1000 and CLPS1000 1.0 mg/ml. Oral administration of Smectite, CLPl000, and CLPS1000 at the dose of 10 mg/ml showed a therapeutic effect for S. typhimurium infected BALB/c mice. The mortality of Smectite, CLP1000 and CLPS1000-treated mice was 90%, 90%, and 70% at 12 days, respectively, while that of untreated mice was 100% at 9 days after a lethal dose of S. typhimurium infection. The results of our study strongly indicate that CLPS1000 has potential as an effective of salmonellosis.

본 연구는 황련, 금은화, 그리고 백작약 혼합분말(1:1:1)의 메탄올 추출물(CLP1000)과, 이에 dioctahedral smectite를 혼합한 합제 (CLPS1000)의 살모넬라에 대한 항균효과를 평가하기 위해 수행되었다. CLP1000와 CLPS1000는, 0. 5mg/ml 농도에서 S. typhimurium에 대한 항균효과를 보이지 않았으나, 1.0 mg/ml의 농도에서는 S. typhimurium에 대한 항균활성이 관찰되었다. BALB/c 마우스를 이용한 살모넬라 감염시험에서 Smectite, CLP1000 그리고 CLPS1000을 각각 10 mg/ml 농도로 12일 동안 투여한 결과, 사망률이 각각 90%, 90% 그리고 70%을 보여, CLPS1000이 마우스 살모넬라증에 강력한 효과를 갖고 있는 것으로 나타났다.

Keywords

References

  1. Cleaveland, S., Laurenson, M.K., Taylor, L.H.: Diseases of humans and their domestic mammals: pathogen characteristics, host range and the risk of emergence. B. Biol. Sci. 356, 991-999 (2001).
  2. Kim, G.S., Kim, D.H., Lim, J.J., Lee, J.J., Han, D.Y., Lee, W.M., Jung, W.C., Min, W.G., Won, C.G., Rhee, M.H., Lee, H.J., Kim, S.: Biological and antibacterial activities of the natural herb Houttuynia cordata water extract against the intracellular bacterial pathogen Salmonella within the raw 64.7 macrophage. Biol. Pharm. Bull. 31, 2012-2017 (2009).
  3. Kim, D.H., Lim, J.J., Lee, J.J., Jung, W.C., Shin, H.J., Lee, H.J., Kim, G.S., Kim, S.: Antibacterial and therapeutic effects of houttuynia cordata ethanol extract for murine salmonellosis. Kor. J. Environ. Agricul. 27, 156-162 (2008). https://doi.org/10.5338/KJEA.2008.27.2.156
  4. Valle, E., Guiney, D.G.: Characterization of salmonella-induced cell death in human macrophage-like THP-1 cells. Infection and. Immunity 73, 2835-2840 (2005). https://doi.org/10.1128/IAI.73.5.2835-2840.2005
  5. Angulo, F.J., Johnson, K.R., Tauxe, R.V., Cohen, M.L.: Origins and consequences of antimicrobial-resistant nontyphoidal Salmonella: implications for the use of fluoroquinolones in food animals. Microb. Drug Resist. 6, 77-83 (2000). https://doi.org/10.1089/mdr.2000.6.77
  6. Gross, U., Tschape, H., Bednarek, I., Frosch, M.: Antibiotic resistance in Salmonella enterica serotype Typhimurium. Eur. J. Clin. Microbiol. Infect. Dis. 17, 385-387 (1998).
  7. O'Brien, T.F.: Emergence, spread, and environmental effect of antimicrobial resistance: how use of an antimicrobial anywhere can increase resistance to any antimicrobial anywhere else. Clin. Infect. Dis. 34, S78-S84 (2002). https://doi.org/10.1086/340244
  8. Merken, H.M., Merken, C.D., Beecher, G.R.: Kinetics method for the quantitation of athocyanidins, flavonols, and flavones in foods. J. Agric. Food Chem. 49, 2727-2732 (2001). https://doi.org/10.1021/jf001266s
  9. Zheng, W., Wang, S.Y.: Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food Chem. 49, 5165-5170 (2001). https://doi.org/10.1021/jf010697n
  10. Tang, J., Feng, Y., Tsao, S., Wang, N., Curtain, R., Wang, Y.: Berberine and Coptidis rhizoma as novel antineoplastic agents: a review of traditional use and biomedical investigations. J. Ethnopharmacol. 126, 5-17 (2009). https://doi.org/10.1016/j.jep.2009.08.009
  11. Iizuka, N., Miyamoto, K., Hazama, S., Yoshino, S., Yoshimura, K., Okita, K., Fukumoto, T., Yamamoto, S., Tangoku, A., Oka, M.: Anticachectic effects of Coptidis rhizoma, an antiinflammatory herb, on esophageal cancer cells that produce interleukin 6. Cancer Lett. 158, 35-41 (2000). https://doi.org/10.1016/S0304-3835(00)00496-1
  12. Son, D.H., Lee, S.I., Chung, Y.G.: Antioxidative of medicinal plants on pathogenic bacteria. J. Korean Soc. Hygiene Sci. 7, 103-108 (2001).
  13. Kwon, H.A., Kwon, Y.J., Kwon, D.Y., Lee, J.H.: Evaluation of antibacterial effects of a combination of Coptidis Rhizoma, Mume Fructus, and Schizandrae Fructus against Salmonella. Int. J. Food Microbiol. 127, 180-183 (2008). https://doi.org/10.1016/j.ijfoodmicro.2008.06.020
  14. Bae, J.H., Lee, SM.: Identification of antimicrobial substances from Prunus mume on the growth of food-borne pathogens. Food Sci. Biotechnol. 12, 128-132 (2003).
  15. Yun, Y.G., Kim, G.M., Lee, S.J., Ryu, S.H., Jang, S.I.: Inhibitory effect of aqueous extract from Lonicera japonica flower on LPS-induced inflammatory mediators in RAW 264.7 macrophages. Kor. J. Herbology 22, 117-125 (2007).
  16. Park, U.Y., Chang, D.S., Cho, H.R.: Screening of antimicrobial activity for medical herb extracts. J. Korean Soc. Food Nutr. 21, 91-96 (1992).
  17. Tae, J., Han, S.W., Yoo, J.Y., Kim, J.A., Kang, O.H., Baek, O.S., Lim, J.P., Kim, D.K., Kim, Y.H., Bae, K.H., Lee, Y.M.: Anti-inflammatory effect of Lonicera japonica in proteinaseactivated receptor 2-mediated paw edema. Clin. Chim. Acta 330, 165-171 (2003). https://doi.org/10.1016/S0009-8981(03)00017-2
  18. Choi, H.Y.: Antimicrobial activity of Paeonia japonica extract and its quality characteristic effects in Sulgidduk. Korean J. Food Cookery Sci. 25, 435-444 (2009).
  19. Kang, S.S., Kim, W.K., Park, K.K., Cho, Y.H., Park, H.S.: Platelet anti-aggregation of Paeony root. Kor. J. Pharmacogn. 22, 215-218 (1991).
  20. Bang, M.H., Song, J.C., Lee, S.Y., Park, N.K., Baek, N.I.: Isolation and structure determination of antioxidants from the root of Paeonia lactiflora. J. Korean Soc. Agric. Chem. Biotechnol. 42, 170-175 (1991).
  21. Kim, J., Kim, H.S.: The immunomodulating effects of the supplementation of Paeonia Japonica extracts in mice. Nutr. Sci. 5, 60-67 (2002).
  22. Jeong, I.Y., Lee, J.S., Oh, H., Jung, U.H., Park, H.R., Jo, S.K.: Inhibitory effect of hot-water extract of Paeonia japonica on oxidative stress and identification of its active components. J. Korean Soc. Food Sci. Nutr. 32, 739-744 (2003). https://doi.org/10.3746/jkfn.2003.32.5.739
  23. Muragami, N., Saka, M., Shimada, H., Matsuda, H., Yamahara, J., Yoshikawa, M.: New bioactive monoterpene glycosides from Paeoniae radix. Chem. Pharm. Bull. 44, 1279- 1281 (1996). https://doi.org/10.1248/cpb.44.1279
  24. Abrahams, P.W.: Soils: their implications to human health. Sci. Total Environ. 291, 1-32 (2002). https://doi.org/10.1016/S0048-9697(01)01102-0
  25. Aufreiter, S., Mahaney, W.C., Milner, M.W., Huffman, M.A., Hancock, R.G., Wink, M., Reich, M.: Mineralogical and chemical interactions of soils eaten by chimpanzees of the Mahale Mountains and Gombe Stream National Parks, Tanzania. J. Chem. Ecol. 27, 285-311 (2001). https://doi.org/10.1023/A:1005628405321
  26. Haydel, S.E., Remenih, C.M., Williams, L.B.: Broad-spectrum in vitro antibacterial activities of clay minerals against antibiotic-susceptible and antibiotic-resistant bacterial pathogens. J. Antimicrob. Chemother. 61, 353-361 (2008).
  27. Chang, F.Y., Lu, C.L., Chen, C.Y., Luo, J.C.: Efficacy of dioctahedral smectite in treating patients of diarrhea-predominant irritable bowel syndrome. J. Gastroenterol. Hepatol. 22, 2266-2272 (2007). https://doi.org/10.1111/j.1440-1746.2007.04895.x
  28. Szajewska, H., Dziechciarz, P., Mrukowicz, J.: Meta-analysis: Smectite in the treatment of acute infectious diarrhoea in children. Aliment. Pharmacol. Ther. 23, 217-227 (2006). https://doi.org/10.1111/j.1365-2036.2006.02760.x
  29. Martirosian, G., Rouyan, G., Zalewski, T., Meisel-Mikolajczyk, F.: Dioctahedral smectite neutralization activity of Clostridium difficile and Bacteroides fragilis toxins in vitro. Acta Microbiol. Pol. 47, 177-183 (1998).
  30. Kim, J.D.: The growth inhibition effect of E. coli KCTC 1039 by combination of natural products bearing antioxdative capacity. Korean J. Biotechnol. Bioeng. 17, 490-496 (2002).
  31. Park, E., Kum, S., Wang, C., Park, S.Y., Kim, B.S., Schuller- Levis, G.: Anti-inflammatory activity of herbal medicines: inhibition of nitric oxide production and tumor necrosis factor- $\alpha$ secretion in an activated macrophage-like cell line. Am. J. Chin. Med. 33, 415-424 (2005). https://doi.org/10.1142/S0192415X05003028