KSBB Journal

  • 제25권2호
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  • Pages.199-204
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  • 2010
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Microchip상에서 효율적인 DNA 분석을 위한 반복단위 단백질의 생산

Production of Repetitive Polypeptides for an Efficient DNA Analysis on a Microchip

  • 이현진 (홍익대학교 화학공학과) ;
  • 최석진 (한국과학기술원 생명화학공학과) ;
  • 서태석 (한국과학기술원 생명화학공학과) ;
  • 원종인 (홍익대학교 화학공학과)
  • Yi, Hyeon-Jin (Department of Chemical Engineering, Hongik University) ;
  • Choi, Seok-Jin (Department of Chemical and Biomolecular Engineering (BK21 program) and Institute for the BioCentry, KAIST) ;
  • Seo, Tae-Seok (Department of Chemical and Biomolecular Engineering (BK21 program) and Institute for the BioCentry, KAIST) ;
  • Won, Jong-In (Department of Chemical Engineering, Hongik University)
  • 투고 : 2010.03.02
  • 심사 : 2010.04.21
  • 발행 : 2010.04.28
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초록

Drag-tag으로 사용될 반복단위 단백질을 생물학적인 방법을 통해 생산함으로써 수용액 내에서 DNA 분리가 가능함을 확인하였다. 서로 다른 크기를 갖는 두 종류의 반복단위 단백질을 디자인하였고, 이를 발현시킨 뒤 정제하였다. 정제된 반복단위 단백질에 형광 dye를 포함하고 있는 100 base의 DNA를 연결하였고, 이 연결 물질을 모세관 내부가 수용액으로 충진된 microchip 상에서 전기영동 하였다. 그 결과 생물학적으로 생산된 반복단위 단백질이 SNP 분석과 같은 빠르고 효율적인 DNA 분석에 적합한 후보물질로 사용될 수 있음을 확인하였다.

We generated the feasibility of DNA separation in free-solution using genetically engineered repetitive polypeptides as drag-tags. Two different-sized repetitive polypeptides were designed, expressed in E. coli, and purified. They were conjugated to a fluorescently labeled DNA (100 base), and the electrophoretic mobilities of these conjugate molecules were analyzed on a microchip. The results of these studies indicate that genetically engineered repetitive polypeptide is a prominent candidate for rapid and high-throughput genetic mutation detection, such as SNP analysis.

키워드

참고문헌

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