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Production of Repetitive Polypeptides for an Efficient DNA Analysis on a Microchip  

Yi, Hyeon-Jin (Department of Chemical Engineering, Hongik University)
Choi, Seok-Jin (Department of Chemical and Biomolecular Engineering (BK21 program) and Institute for the BioCentry, KAIST)
Seo, Tae-Seok (Department of Chemical and Biomolecular Engineering (BK21 program) and Institute for the BioCentry, KAIST)
Won, Jong-In (Department of Chemical Engineering, Hongik University)
Publication Information
KSBB Journal / v.25, no.2, 2010 , pp. 199-204 More about this Journal
Abstract
We generated the feasibility of DNA separation in free-solution using genetically engineered repetitive polypeptides as drag-tags. Two different-sized repetitive polypeptides were designed, expressed in E. coli, and purified. They were conjugated to a fluorescently labeled DNA (100 base), and the electrophoretic mobilities of these conjugate molecules were analyzed on a microchip. The results of these studies indicate that genetically engineered repetitive polypeptide is a prominent candidate for rapid and high-throughput genetic mutation detection, such as SNP analysis.
Keywords
repetitive polypeptide; end-labeled free-solution electrophoresis (ELFSE); drag-tag; capillary electrophoresis; microchip;
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Times Cited By KSCI : 1  (Citation Analysis)
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