비듬유발균 (Pityrosporum ovale) 에 대한 은행잎으로부터 추출한 Ginkgolide 및 Bilobalide의 항진균 효과

Antifungal Effect of Bilobalide and Ginkgolide Extracted from Leaves of Ginkgo biloba Against Pityrosporum ovale

  • 이인화 (조선대학교 환경공학과 BK21 바이오가스기반 수소생산 전문인력양성팀) ;
  • 김미진 (조선대학교 환경공학과 BK21 바이오가스기반 수소생산 전문인력양성팀) ;
  • 최준호 (조선대학교 환경공학과 BK21 바이오가스기반 수소생산 전문인력양성팀) ;
  • 김치현 (조선대학교 환경공학과 BK21 바이오가스기반 수소생산 전문인력양성팀) ;
  • 최승현 ((주)식물나라)
  • Lee, In-Hwa (Department of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University) ;
  • Kim, Mi-Jin (Department of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University) ;
  • Choi, Jun-Ho (Department of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University) ;
  • Kim, Chi-Hyun (Department of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University) ;
  • Choi, Seung-Hyun (Phytoworld Co., Ltd.)
  • 투고 : 2009.12.30
  • 심사 : 2010.04.20
  • 발행 : 2010.04.28

초록

Antifungal effect of Ginkgo biloba leaves extracts conducted for Pityrosporum ovale. Antifungal effect verified by diffusion test, optical density test and colony counting test under various concentration. Extract of ginkgo biloba leaves performed with 40% ethanol and 60% water solution at $60^{\circ}C$ and major components analyzed by HPLC. The concentrated extract have bilobalide and ginkgolide A and ginkgolide B and their concentration were 153.0 mg/L, 8403.5 mg/L and 2723.0 mg/L respectively. Ginkgo biloba leaves extracts gave 99.1% of antifungal effect for Pityrosporum ovale examined by colony counting method.

키워드

참고문헌

  1. Seo, S. B., S. R, Jung, and S. H. Oh (1990) Distribution of causative fungi in home environment of parients with tinea capitis caused bu marcrosporum canis. Korean J. Dermatol. 28: 165-173.
  2. Hang, S. O. (2002) Thnea capitis in a postmenopausal woman. Korean J. Dermatol. 38: 556-558.
  3. McGinley, K. J., J. J. Leyden, and R. R. Marples (1979) Quantitative microbiology of the scalp in non-dandruff, dandruff and seborrheic dermatitis. J. Invest. Dermatol. 64: 401-405.
  4. Lee, H. K. (2003) A Study on the Effect of Natural Essential Oil in Dandruff Germ. A Master's Thesis. University of Yongin, Korea.
  5. Jung, H. J., D. W. Kim, J. B. Jun, and S. L. Chung, (1993) Statistical and mycologic studies on dandruff. Korean J. Dermatol. 31: 164-174.
  6. Textbook Compilation Committee (2001) Dematology. Korean Dermatological Association. p. 325. Yeomongak Press, Korea.
  7. Lee, K. J. and J. M. Kwon (1998) Antimicrobial activity of zirconium pyrithione complex. Korean J. Pharm. Sci. 18: 107-111.
  8. Jang, S. Y., S. Y. Ryu, and S. D. Kim (2003) Antifungal activity of plant extracts against Pityrosporum ovale and Candida albicans. Kor. J. Pharmacogn. 34: 303-307.
  9. Nelson, J. D. and G. A. Hyde (1981) Sodium and zinc omadine antimicrobials as cosmetic preservative. Cosmetics Toiletries 96: 87-90.
  10. Lin, C. C. S. and D. Y. C. Fung (1983) Effect of BHA, BHT, TBHQ and PG on growth and toxigenesis of selected Aspergilli. J. Food Sci. 48: 578-583.
  11. Mazzanti, G., M. T. Mascellino, L. Battinelli, D. Coluccia, M. Manganaro, and L. Saso (2003) Antimicrobial investigation of semipurified factions of Ginkgo biloba leaves. J. Ethnopharmacol. 71: 83-88.
  12. Boonkaew, T. and N. D. Camper (2005) Biological activities of ginkgo extracts. Phytomedicine 12: 318-323. https://doi.org/10.1016/j.phymed.2003.06.008
  13. Huang, X., W. J. Xie, and Z. Z. Gong (2002) Characteristics and antifungal activity of a chitin binding protein from Ginkgo biloba, FEBS Letters 478: 123-126.
  14. Jaracz, S., S. Malik, and K. Nakanishi (2004) Isolation of ginkgolides A, B, C, J and bilobalide from G. biloba extracts. Phytochem. 65: 2897-2902. https://doi.org/10.1016/j.phytochem.2004.08.026
  15. Tang, C., X. Wei, and C. Yin (2003) Analysis of ginkgolides and bilobalide in Ginkgo biloba L. extract injections by high-performance liquid chromatography with evaporative light scattering detection. J. Pharm. Biomed. Anal. 33: 811-817. https://doi.org/10.1016/S0731-7085(03)00309-1
  16. Deng, F. and S. W. Zito (2003) Development and validation of a gas chromatographic-mass spectrometric method for simultaneous identification and quantification of maker compounds including bilobalide, ginkgolides and flavonoids in Ginkgo biloba L. extract and pharmaceutical preparations. J. Chromatogr. A. 986: 121-127. https://doi.org/10.1016/S0021-9673(02)01921-0
  17. Cheung, F., Y. L. Siow, and O. Karmin (2001) Inhibition by ginkbolides and bilobalide of the production of nitric oxide in macrophages (THP-1) but not in endothelial cells (HUVEC). Biochem Pharmacol. 61: 503-510. https://doi.org/10.1016/S0006-2952(00)00567-0
  18. Arredondo, M. F., F. Blasina, C. Echeverry, A. Morquio, M. Ferreira, J. A. Abin-Carriquiry, L. Lafon, and F. Dajas (2004) Cytoprotection by achyrocline satureioides (Lam) D.C. and some of its main flavonoids against oxidative stress. J. Ethnophamacology 91: 13-20. https://doi.org/10.1016/j.jep.2003.11.012
  19. Kim, S. I., C. Park, M. H. Oh, H. C. Cho, and Y. J. Ahn (2003) Conact and fumigant activities of aromatic plant extracts and essential oils against Lasioderma serricorne (Coleoptera: Anobiidae). J. Stored Prod. Res. 39: 11-19. https://doi.org/10.1016/S0022-474X(02)00013-9
  20. Suzuki, R., H. Sugie, K. Sasaki, T. Yoshimura, K. Wada, and T. Tanaka (2004) Preventive effects of extract of leaves of ginkgo (Ginkgo biloba) and its component bilobalide on azoxymethane-induced colonic aerrant crypt foci in rats. Cancer Lett. 210: 159-169. https://doi.org/10.1016/j.canlet.2004.01.034
  21. Chen, Y. S., C. J. Liu, C. Y. Cheng, and C. H. Yao (2004) Effect of bilobalide on peripheral nerve regeneration. Biomaterials 25: 509-514. https://doi.org/10.1016/S0142-9612(03)00548-9
  22. Lee, I. W., S. Yoon, S. H. Choi, J. Y. Park, S. Y. Han, J. Y. Song, and S. J. Yoon (2006) A study in the antimicrobial effect of Ginkgo biloba leaves extracts according to concentration of ethanol for Staphylococcus aureus. Korean J. Biotechnol. Bioeng. 21: 312-316.