Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Effect of Platycodin D on Airway MUC5AC Mucin Production and Gene Expression Induced by Growth Factor and Proinflammatory Factor

  • Lee, Hyun-Jae (Department of Pharmacology, School of Medicine, Chungnam National University) ;
  • Lee, Su-Yel (Department of Pharmacology, School of Medicine, Chungnam National University) ;
  • Jeon, Byeong-Kyou (Department of Radiologic Technology, Daegu Health College) ;
  • Lee, Jae-Woo (LG Life Science) ;
  • Kim, Young-Sik (Department of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Lee, Mi-Nam (Department of Pharmacology, School of Medicine, Chungnam National University) ;
  • Lee, Choong-Jae (Department of Pharmacology, School of Medicine, Chungnam National University)
  • Received : 2010.07.08
  • Accepted : 2010.07.19
  • Published : 2010.07.31
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Abstract

In this study, we tried to investigate whether platycodin D significantly affects MUC5AC mucin production and gene expression induced by epidermal growth factor (EGF), phorbol ester (PMA) and tumor necrosis factor-$\alpha$ (TNF-$\alpha$) from human airway epithelial cells. Confluent NCI-H292 cells were pretreated with varying concentrations of platycodin D for 30 min and then stimulated with EGF, PMA and TNF-$\alpha$ for 24h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA. The results were as follows: (1) Platycodin D was found to inhibit the production of MUC5AC mucin protein induced by EGF, PMA, and TNF-$\alpha$, respectively. (2) It also inhibited the expression of MUC5AC mucin gene induced by the same inducers. These results suggest that platycodin D can regulate mucin gene expression and production of mucin protein, by directly acting on human airway epithelial cells.

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References

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