[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4062/biomolther.2010.18.3.294

Effect of Platycodin D on Airway MUC5AC Mucin Production and Gene Expression Induced by Growth Factor and Proinflammatory Factor

Lee, Hyun-Jae (Department of Pharmacology, School of Medicine, Chungnam National University)
Lee, Su-Yel (Department of Pharmacology, School of Medicine, Chungnam National University)
Jeon, Byeong-Kyou (Department of Radiologic Technology, Daegu Health College)
Lee, Jae-Woo (LG Life Science)
Kim, Young-Sik (Department of Pharmaceutical Sciences, College of Pharmacy, Seoul National University)
Lee, Mi-Nam (Department of Pharmacology, School of Medicine, Chungnam National University)
Lee, Choong-Jae (Department of Pharmacology, School of Medicine, Chungnam National University)

Publication Information

Biomolecules & Therapeutics / v.18, no.3, 2010 , pp. 294-299 More about this Journal

Abstract

In this study, we tried to investigate whether platycodin D significantly affects MUC5AC mucin production and gene expression induced by epidermal growth factor (EGF), phorbol ester (PMA) and tumor necrosis factor- $\alpha$ (TNF- $\alpha$ ) from human airway epithelial cells. Confluent NCI-H292 cells were pretreated with varying concentrations of platycodin D for 30 min and then stimulated with EGF, PMA and TNF- $\alpha$ for 24h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA. The results were as follows: (1) Platycodin D was found to inhibit the production of MUC5AC mucin protein induced by EGF, PMA, and TNF- $\alpha$ , respectively. (2) It also inhibited the expression of MUC5AC mucin gene induced by the same inducers. These results suggest that platycodin D can regulate mucin gene expression and production of mucin protein, by directly acting on human airway epithelial cells.

Keywords

Airway mucin; MUC5AC; Platycodin D;

Citations & Related Records

Times Cited By KSCI : 1 (Citation Analysis)
Times Cited By Web Of Science : 0 (Related Records In Web of Science)
Times Cited By SCOPUS : 0

Reference
Cited By KSCI

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1	Apigenin and Wogonin Regulate Epidermal Growth Factor Receptor Signaling Pathway Involved in MUC5AC Mucin Gene Expression and Production from Cultured Airway Epithelial Cells / [Sikder, Md. Asaduzzaman;Lee, Hyun Jae;Ryu, Jiho;Park, Su Hyun;Kim, Ju-Ock;Hong, Jang-Hee;Seok, Jeong Ho;Lee, Choong Jae;] / Tuberculosis and Respiratory Diseases
2	Effect of Prunetin on TNF- ${\alpha}$ -Induced MUC5AC Mucin Gene Expression, Production, Degradation of $I{\kappa}B$ and Translocation of NF- ${\kappa}B$ p65 in Human Airway Epithelial Cells / [Ryu, Jiho;Lee, Hyun Jae;Park, Su Hyun;Sikder, Md. Asaduzzaman;Kim, Ju-Ock;Hong, Jang-Hee;Seok, Jeong Ho;Lee, Choong Jae;] / Tuberculosis and Respiratory Diseases