Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Effect of Atrazine, Perfluorooctanoic Acid and Zearalenone on IFNγ, TNFα, and IL-5 mRNA Expression in Jurkat Cells

  • Lee, Sung-Woo (College of Veterinary Medicine, Chungnam National University) ;
  • Son, Hwa-Young (College of Veterinary Medicine, Chungnam National University) ;
  • Yoon, Won-Kee (Korea Research Institute of Bioscience and Biotechnology) ;
  • Jung, Ju-Young (College of Veterinary Medicine, Chungnam National University) ;
  • Park, Bae-Keun (College of Veterinary Medicine, Chungnam National University) ;
  • Cho, Eun-Sang (College of Veterinary Medicine, Chungnam National University) ;
  • Park, Sang-Joon (College of Verinary Medicine, Kyungpook National University) ;
  • Kim, Tae-Hwan (College of Verinary Medicine, Kyungpook National University) ;
  • Ryu, Si-Yun (College of Veterinary Medicine, Chungnam National University)
  • Received : 2010.06.14
  • Accepted : 2010.07.01
  • Published : 2010.07.31
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Abstract

Cytokine production is a sensitive indicator for monitoring perturbations of the immune system by xenobiotics in animals and humans. In the present study, we evaluated the changes in $IFN{\gamma}$, IL-5 and $TNF{\alpha}$ mRNA expression after atrazine (ATZ), perfluorooctanoic acid (PFOA) or zearalenone (ZEA) exposure in Jurkat cells. The IC50 (concentration for a 50% inhibition of cell proliferation) of PFOA and ZEA after 3 days culture were $226.6\;{\mu}M$ and $52.6\;{\mu}M$, respectively. The effects of ATZ on cytokine expression followed in increasing order of $IFN{\gamma}$>IL-5>$TNF{\alpha}$ at $3\;{\mu}M$ and at the lower concentrations the degree of effects on three cytokines were less clear between the cytokines when compared to control level. PFOA had marked increasing effect in order of $IFN{\gamma}$>$TNF{\alpha}$>IL-5 mRNA expression at IC50, and these patterns were continued at the lower concentrations, IC50/2 and IC50/4. ZEA caused the overexpression of cytokine mRNAs in order of IL-5>$IFN{\gamma}$>$TNF{\alpha}$ at both IC50 and IC50/2, and at IC50/4 the overexpression order was IL-5>$TNF{\alpha}$. On other hand, $IFN{\gamma}$ was less distinct compared to the control. These data indicate that ATZ, PFOA and ZEA caused the overtranscription of $IFN{\gamma}$, IL-5 and $TNF{\alpha}$ mRNA, and the overproduction of these cytokines may eventually lead to immune disorders.

Keywords

References

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