Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Screening of Vietnamese Medicinal Plants for Cytotoxic Activity

  • Received : 2009.12.01
  • Accepted : 2010.03.17
  • Published : 2010.03.31
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Abstract

Thirty-two methanol extracts of thirty-one Vietnamese medicinal plants were evaluated for the cytotoxic activity against five human cancer cell lines, including A549, MCF-7, HT 1080, Huh-7, and HepG2. Of these, the nine extracts of Acanthopanax trifoliatus (4), Acanthopanax gracilistylus (5), Siegesbeckia orientalis (10), Betula alnoides (11), Passiflora edulis (18), Zanthoxylum simulans (leaf, 23), Adenosma caeruleum (26), Solanum verbascifolium (29), and Alpinia malaccensis (31), exhibited high potent cytotoxic activity showing a certain degree of selectivity against the different cell types, with $IC_{50}$ values ranging from 2.1 to $3.8\;{\mu}g/mL$.

Keywords

References

  1. American Cancer Society, Global cancer facts and figures 2007, Atlanta, Georgia, pp. 1-45 (2007).
  2. Barbosa, P.R., Valvassori, S.S., Bordignon, C.L.J., Kappel, V.D., Martins, M.R., Gavioli, E.C., Quevedo, J., and Reginatto, F.H., The aqueous extracts of Passiflora alata and Passiflora edulis reduce anxietyrelated behaviors without affecting memory process in rats. J. Med. Food., 11, 282-288 (2008). https://doi.org/10.1089/jmf.2007.722
  3. Boik, J., Natural Compounds in Cancer Therapy: Promising Nontoxic Antitumor Agents From Plants & Other Natural Sources. Oregon Medical Press, Minnesota, USA, pp. 25 (2001).
  4. Brandin, H., Viitanen, E., Myrberg, O., and Arvidsson, A.K., Effects of herbal medicinal products and food supplements on induction of CYP1A2, CYP3A4 and MDR1 in the human colon carcinoma cell line LS180. Phytother. Res., 21, 239-244 (2007). https://doi.org/10.1002/ptr.2057
  5. Cardellina II, J.H., Fuller, R.W., Gamble, W.R., Westergaard, C., Boswell, J., Munro, M.H.G., Currens, M., and Boyd, M.R., Evolving strategies for the selection, dereplication and prioritization of antitumor and HIV-inhibitory natural products extracts. In: Bohlin, L., Bruhn, J.G. (Eds.), Bioassay Methods in Natural Product Research and Development. Kluwer Academic Publishers, Dordrecht, pp. 25-36 (1999).
  6. Chen, X.C., Xia, L., Hu, S., and Huang, G., Inhibitory effects of Acanthopanax gracilistylus saponins on human platelet aggregation and platelet factor 4 liberation in vitro. Zhongguo Yao Li Xue Bao, 17, 523-526 (1996).
  7. Chi, V.V., Dictionary of Vietnamese medicinal plants. Medical Publishing House, Hanoi, Vietnam (1997).
  8. da Rocha, A.B., Lopes, R.M., and Schwartsmann, G., Natural products in anticancer therapy. Curr. Opin. Pharmacol., 1, 364-336 (2001). https://doi.org/10.1016/S1471-4892(01)00063-7
  9. De Abreu, M.B., Malafronte, N., Van Kiem, P., and Braca, A., A new iridoid from Adenosma caeruleum R. Br. Fitoterapia, 80, 358-360 (2009). https://doi.org/10.1016/j.fitote.2009.05.003
  10. de Mesquita, M.L., de Paula, J.E., Pessoa, C., de Moraes, M.O., Costa- Lotufo, L.V., Grougnet, R., Michel, S., Tillequin, F., and Espindola, L.S., Cytotoxic activity of Brazilian Cerrado plants used in traditional medicine against cancer cell lines. J. Ethnopharmacol., 123, 439-445 (2009). https://doi.org/10.1016/j.jep.2009.03.018
  11. Frederiksen, H., Mortensen, A., Schroder, M., Frandsen, H., Bysted, A., Knuthsen, P., and Rasmussen, S.E., Effects of red grape skin and seed extract supplementation on atherosclerosis in Watanabe heritable hyperlipidemic rabbits. Mol. Nutr. Food Res., 51, 564-571 (2007). https://doi.org/10.1002/mnfr.200700009
  12. Giang, P.M., Son, P.T., and Otsuka, H., Ent-pimarane-type diterpenoids from Siegesbeckia orientalis L. Chem. Pharm. Bull., 53, 232-234 (2005). https://doi.org/10.1248/cpb.53.232
  13. Hwang, W.J., Park, E.J., Jang, C.H., Han, S.W., Oh, G.J., Kim, N.S., and Kim, H.M., Inhibitory effect of immunoglobulin E production by jindeuk- chal (Siegesbeckia orientalis). Immunopharmacol. Immunotoxicol., 23, 555-563 (2001). https://doi.org/10.1081/IPH-100108601
  14. Joy, S., Siow, R.C., Rowlands, D.J., Becker, M., Wyatt, A.W., Aaronson, P.I., Coen, C.W., Kallo, I., Jacob, R., and Mann, G.E., The Isoflavone Equol Mediates Rapid Vascular Relaxation Ca2+ independent activation of endothelial nitric-oxide synthase/Hsp90 involving ERK1/ 2 and Akt phosphorylation in human endothelial cells. J. Biol. Chem., 281, 27335-27345 (2006). https://doi.org/10.1074/jbc.M602803200
  15. Kaileh, M., Berghe, W.V., Boonec, E., and Essawi, T., and Haegeman, G., Screening of indigenous Palestinian medicinal plants for potential anti-inflammatory and cytotoxic activity. J. Ethnopharmacol., 113, 510-516 (2007). https://doi.org/10.1016/j.jep.2007.07.008
  16. Kamperdick, C., Thuy, T.T., Van, S.T., and Adam, G., Triterpenoids from Betula alnoides. Planta Med., 61, 486 (1995).
  17. Kiem, P.V., Cai, X.F., Minh, C.V., Lee, J.J., and Kim, Y.H., Lupanetriterpene carboxylic acids from the leaves of Acanthopanax trifolius. Chem. Pharm. Bull., 51, 1432-1435 (2003a). https://doi.org/10.1248/cpb.51.1432
  18. Kiem, P.V., Cai, X.F., Minh, C.V., Lee, J.J., and Kim, Y.H., Kaurane-type diterpene glycoside from the stem bark of Acanthopanax trifolius. Planta Med. 70, 282-284 (2004). https://doi.org/10.1055/s-2004-818926
  19. Kiem, P.V., Minh, C.V., Dat, N.T., Cai, X.F., Lee, J.J., and Kim, Y.H., Two new phenylpropanoid glycosides from the stem bark of Acanthopanax trifolius. Arch. Pharm. Res., 26, 1014-1017 (2003b). https://doi.org/10.1007/BF02994751
  20. Lee, Y.S., Kim, W.S., Kim, K.H., Yoon, M.J., Cho, H.J., Shen, Y., Ye, J.M., Lee, C.H., Oh, W.K., Kim, C.T., Hohnen-Behrens, C., Gosby, A., Kraegen, E.W., James, D.E., and Kim, J.B., Berberine, a natural plant product, activates AMP-activated protein kinase with beneficial metabolic effects in diabetic and insulin-resistant states. Diabetes, 55, 2256-2264 (2006). https://doi.org/10.2337/db06-0006
  21. Liu, X.Q., Chang, S.Y., Park, S.Y., Nohara, T., and Yook, C.S., A new lupane-triterpene glycoside from the leaves of Acanthopanax gracilistylus. Arch. Pharm. Res. 25, 831-836 (2002). https://doi.org/10.1007/BF02977000
  22. Loi, D.T., Vietnamese Medicinal Plants and Ingredients. Medical Publishing House, Hanoi, Vietnam (2004).
  23. Mann, J., Natural products in cancer chemotherapy: past, present and future. Nat. Rev.: Cancer, 2, 143-148 (2002).
  24. Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65, 55-63 (1983). https://doi.org/10.1016/0022-1759(83)90303-4
  25. Nam, N.H., Kim, H.M., Bae, K.H., and Ahn, B.Z., Inhibitory effects of Vietnamese medicinal plants on tube-like formation of human umbilical venous cells. Phytother. Res., 17, 107-111 (2003). https://doi.org/10.1002/ptr.934
  26. Newman, D.J. and Cragg, G.M., Natural products in drug discovery and development. J. Nat. Prod., 60, 52-60 (1997). https://doi.org/10.1021/np9604893
  27. Newman, D.J., Cragg, G.M., 2007. Natural products in drug discovery and development. J. Nat. Prod. 70, 461-477. https://doi.org/10.1021/np068054v
  28. Newman, D.J., Cragg, G.M., and Snader, K.M., Natural Products as Sources of New Drugs over the Period 1981-2002. J. Nat. Prod., 66, 1022-1037 (2003). https://doi.org/10.1021/np030096l
  29. Perry, L.M. and Metzger, J., Medicinal Plants of East and Southeast Asia. MIT Press, Cambridge, Massachusetts, USA (1980).
  30. Sena, L.M., Zucolotto, S.M., Reginatto, F.H., Schenkel, E.P., and De Lima, T.C., Neuropharmacological activity of the pericarp of Passiflora edulis flavicarpa degener: putative involvement of Cglycosylflavonoids. Exp. Biol. Med., 234, 967-975 (2003).
  31. Shan, B.E., Fu, X.M., Hua, Z.X., Li, Q., Liang, W., Liu, J., Zhang, H., and Liu, G., Study on mechanism of the anti-tumor activity of Acanthopanax gracilistylus. Zhongguo Zhong Xi Yi Jie He Za Zhi, 25, 825-828 (2005).
  32. Shan, B.E., Yoshita, Y., Sugiura, T., and Yamashita, U., Suppressive effect of Chinese medicinal herb, Acanthopanax gracilistylus, extract on human lymphocytes in vitro. Clin. Exp. Immunol., 118, 41-48 (1999). https://doi.org/10.1046/j.1365-2249.1999.01031.x
  33. Shan, B.E., Zeki, K., Sugiura, T., Yoshida, Y., and Yamashita, U., Chinese medicinal herb, Acanthopanax gracilistylus, extract induces cell cycle arrest of human tumor cells in vitro. Jpn. J. Cancer Res., 91, 383-389 (2000). https://doi.org/10.1111/j.1349-7006.2000.tb00956.x
  34. Sur, T.K., Pandit, S., Battacharyya, D., Kumar, C.K., Lakshmi, S.M., Chatttopadhyay, D., and Mandal, S.C., Studies on the antiinflammatory activity of Betula alnoides bark. Phytother. Res., 16, 669-671 (2002). https://doi.org/10.1002/ptr.942
  35. Walgren, R.A., Karnaky, K.J.J., Lindenmayer, G.E., and Walle, T., Efflux of dietary flavonoid quercetin 4'-beta-glucoside across human intestinal Caco-2 cell monolayers by apical multidrug resistanceassociated protein-2. J. Pharmacol. Exp. Ther., 294, 830-836 (2000).
  36. Wang, F., Cheng, X.L., Li, Y.J., Shi, S., and Liu, J.K., ent-Pimarane diterpenoids from Siegesbeckia orientalis and structure revision of a related compound. J. Nat. Prod., 72, 2005-2008 (2009). https://doi.org/10.1021/np900449r
  37. WHO, "Cancer" World Health Organization (2006).
  38. Xiang, Y., Zhang, H., Fan, C.Q., and Yue, J.M., Novel diterpenoids and diterpenoid glycosides from Siegesbeckia orientalis. J. Nat. Prod., 67, 1517-1521 (2004). https://doi.org/10.1021/np0400407
  39. Yan, L.P., Chan, S.W., Chan, A.S., Chen, S.L., Ma, X.J., and Xu, H.X., Puerarin decreases serum total cholesterol and enhances thoracic aorta endothelial nitric oxide synthase expression in diet-induced hypercholesterolemic rats. Life Sci., 79, 324-330 (2006). https://doi.org/10.1016/j.lfs.2006.01.016
  40. Yang, Y.P., Cheng, M.J., Teng, C.M., Chang, Y.L., Tsai, I.L., and Chen, I.S., Chemical and anti-platelet constituents from Formosan Zanthoxylum simulans. Phytochemistry, 61, 567-572 (2002) https://doi.org/10.1016/S0031-9422(02)00268-6
  41. Yook, C.S., Liu, X.Q., Chang, S.Y., Park, S.Y., and Nohara, T., Lupanetriterpene glycosides from the leaves of Acanthopanax gracilistylus. Chem. Pharm. Bull. 50, 1383-1385 (2002). https://doi.org/10.1248/cpb.50.1383
  42. Zucolotto, S.M., Goulart, S., Montanher, A.B., Reginatto, F.H., Schenkel, E.P., and Fröde, T.S., Bioassay-guided isolation of anti-inflammatory C-glucosylflavones from Passiflora edulis. Planta Med., 75, 1221-1226 (2009). https://doi.org/10.1055/s-0029-1185536