약학회지 (YAKHAK HOEJI)

  • 제54권6호
  • /
  • Pages.522-528
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  • 2010
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  • 0377-9556(pISSN)
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  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

난소절제 랫트에서 에스트로겐 저하가 알코올 대사에 미치는 영향

The Effect of Estrogen Deficiency on Ethanol Metabolism in Ovariectomized Rats

  • Chang, Bo-Yoon (College of Pharmacy, Institute of Pharmaceutical Research and Development, Wonkwang University) ;
  • Kim, Sung-Yeon (College of Pharmacy, Institute of Pharmaceutical Research and Development, Wonkwang University)
  • 투고 : 2010.10.21
  • 심사 : 2010.11.30
  • 발행 : 2010.12.31
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초록

This study was designed to examine the effect of estrogen deficiency on the metabolism of ethanol in ovariectomized rats. Female rats were assigned to an ovariectomy (OVX) and a sham (SHAM) surgery group. Gain body weight was greater in incresed in OVX group and especially uterus weight significantly decrease depending on the concentration of estrogen after 3 month of ovariectomy. Ethanol at the tolerative dose (6 g/kg) was injected to rats by oral administration to measure the concentration of ethanol in blood. The area under the blood concentration time curve (AUC) was significantly lower in OVX group than SHAM group. The significant decrease in AUC in OVX group indicates that the estrogen deficiency leads to changes of the factors related to ethanol metabolism. Activity of hepatic alcohol dehydrogenase was not significantly influenced by the ovariectomy and also the ethanol elimination rate in vivo was not different. Cytochrome P450 isozymes did not show any changes except CYP 1A1 and 2E1. Level of hepatic glutathione in OVX group was higher after treatment of ethanol. Therefore the reduction of AUC appears not to be directly associated with the difference of ethanol metabolizing enzyme, but to be related with the physical factors like body weight.

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참고문헌

  1. 통계청 : 인구동채통계연보(혼인편), 통계청, 서울 (2009).
  2. 질병관리본부 : 국민건강영양조사(건강설문조사부분) 질병관리본부, 서울 (2009).
  3. 통계청 : 음주인구비율(구분별), 통계청, 서울 (1995).
  4. Lieber, C. S. : Alcohol and the liver: Gastroenterol. 106, 1085 (1994). https://doi.org/10.1016/0016-5085(94)90772-2
  5. Ramchandani, V. A., Bosron, W. F. and Li, T. K. : Research advances in ethanol metabolism. Pathol. Biol. 49, 676 (2001). https://doi.org/10.1016/S0369-8114(01)00232-2
  6. Joornvall, H., Hoooog, J. O., Bahr-Lindstroom, H., Johanson, J., Kaiser, R. and Person, R. : Alcohol dehydrogenase and aldehyde dehydrogenase. Biochem. Soc. Trans. 16, 223 (1988). https://doi.org/10.1042/bst0160223
  7. Toran-Allerand, C. D. : Gonadal hormones and brain development: implications for the genesis of sexual differentiation. Ann. N Y Acad. Sci. 435, 101 (1984). https://doi.org/10.1111/j.1749-6632.1984.tb13743.x
  8. Mendoza-Nunez, V. M., Beristain-Perez, A., Perez-Vera, S. P. and Altamirano-Lozano, M. A. : Age-related sex differences in glutathione peroxidase and oxidative DNA damage in a healthy Mexican population. J. Womens Health (Larchmt) 19, 919 (2010). https://doi.org/10.1089/jwh.2009.1684
  9. Ann, Macgregor, 이병석역 : Menopuase and HRT, 아카데미아, 서울 p. 26 (2005).
  10. Sato, N., Lindros, K. O., Baraona, E., Ikejima, K., Mezey, E., Järveläinen, H. A. and Ramchandani, V. A. : Sex difference in alcohol-related organ injury. Alcohol. Clin. Exp. Res. 25, 40S (2001). https://doi.org/10.1111/j.1530-0277.2001.tb02371.x
  11. Muller, C. : Liver, alcohol and gender. Wien. Med. Wochenschr. 156, 523 (2006). https://doi.org/10.1007/s10354-006-0348-8
  12. Eagon, P. K. : Alcoholic liver injury: influence of gender and hormones. World J. Gastroenterol. 16, 1377 (2010). https://doi.org/10.3748/wjg.v16.i11.1377
  13. Naresh, C. and Robert, H. : Analysis of alcohol 2. A review of gas chromatogaraphic method. J. Chromatogaraph Sci. 6, 263 (1972).
  14. Koop, D. R., Crump, B. L., Nordblom, G. D. and Coon, M. J. : Immunochemical evidence for induction of the alcoholoxidizing cytochrome P-450 of rabbit liver microsomes by diverse agents: ethanol, imidazole, trichloroethylene, acetone, pyrazole, and isoniazid. Proc. Natl. Acad. Sci. USA 82, 4065 (1985). https://doi.org/10.1073/pnas.82.12.4065
  15. Bradford, M. M. : A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 7, 248 (1976).
  16. Crow, K. E., Braggins, T. J., Batt, R. D. and Hardman, M. J. : Rat liver cytosolic malate dehydrogenase: purification, kinetic properties, role in control of free cytosolic NADH concentration. Analysis of control of ethanol metabolism using computer simulation. J. Biol. Chem. 257, 14217 (1982).
  17. Griffith, O. W. : Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal. Biochem. 106, 207 (1980). https://doi.org/10.1016/0003-2697(80)90139-6
  18. Cole-Harding, S. and Wilson, J. R. : Ethanol metabolism in men and women. Journal of Studies on Alcoholism. 48, 380 (1987). https://doi.org/10.15288/jsa.1987.48.380
  19. Allsworth, J. E., Clarke, J., Peipert, J. F., Hebert, M. R., Cooper A. and Boardman L. A. : The influence of stress on the menstrual cycle among newly incarcerated women. Womens Health Issues. 17, 202 (2007). https://doi.org/10.1016/j.whi.2007.02.002
  20. Holm, A., Andersson, K. E., Nordstrom, I. and Hellstrand, P. : Nilsson BO.Down-regulation of endothelial cell estrogen receptor expression by the inflammation promoter LPS. Mol. Cell. Endocrinol. 319, 8 (2010). https://doi.org/10.1016/j.mce.2010.01.002
  21. Ke, H. Z., Jee, W. S. S., Zeng, Q. Q., Li, M. and Lin, B. Y. : Prostaglandin E2 increased rat cortical bone mass when administered immediately following ovariectomy. Bone Miner. 21, 189 (1993). https://doi.org/10.1016/S0169-6009(08)80230-9
  22. Li, X. X., Hara, I. and Matsumiya, T. : Effects of osthole on postmenopausal osteoporosis using ovariectomized rats; comparison to the effects of estradiol. Biol. Pharm. Bull. 25, 738 (2002). https://doi.org/10.1248/bpb.25.738
  23. Zych, M., Folwarczna, J. and Trzeciak, H. I. : Natural phenolic acids may increase serum estradiol level in ovariectomized rats. Acta. Biochim. Pol. 56, 503 (2009).
  24. Gloria, Rachamin J., Alain, M., Samina, W. and Jeremy, J. : Modulation of alcohol dehydrogenase and ethanol metabolism by sex hormones in the spontaneously hypertensive rat. Effect of chronic ethanol administration. Biochem. J. 15, 483 (1990).
  25. Legros, J. J. : ADH and oxytocin in age-related minor cognitive impairment. Aging (Milano) 9, 36 (1997).
  26. Harada, S., Tachiyashiki, K. and Imaizumi, K. : Effect of sex hormones on rat liver cytosolic alcohol dehydrogenase activity. J. Nutr. Sci. Vitaminol. (Tokyo) 44, 625 (1998). https://doi.org/10.3177/jnsv.44.625
  27. Harper C. and Kril J. : An introduction to alcohol-induced brain damage and its causes. Alcohol. Alcohol. Suppl. 2, 237 (1994).
  28. Hidaka, I., Hino, K., Korenaga, M., Gondo, T., Nishina, S., Ando, M., Okuda, M. and Sakaida, I. : Stronger Neo- Minophagen C, a glycyrrhizin-containing preparation, protects liver against carbon tetrachloride-induced oxidative stress in transgenic mice expressing the hepatitis C virus polyprotein. Liver Int. 27, 845 (2007). https://doi.org/10.1111/j.1478-3231.2007.01492.x
  29. Hamden, K., Carreau, S., Ayadi, F. and Masmoudi, H. : A Inhibitory effect of estrogens, phytoestrogens, and caloric restriction on oxidative stress and hepato-toxicity in aged rats. Biomed. Environ. Sci. 22, 381 (2009). https://doi.org/10.1016/S0895-3988(10)60015-3
  30. Ramirez, M. E., McMurry, M. P., Wiebke, G. A., Felten, K. J., Ren, K., Meikle, A. W. and Iverius P. H. : Evidence for sex steroid inhibition of lipoprotein lipase in men: comparison of abdominal and femoral adipose tissue. Metabolism. 46, 179 (1997). https://doi.org/10.1016/S0026-0495(97)90299-7
  31. Gandhi, M., Aweeka, F., Greenblatt, R. M. and Blaschke, T. F. : Sex differences inpharmacokinetics and pharmacodynamics. Annu. Rev. Pharmacol. Toxicol. 44, 499 (2004). https://doi.org/10.1146/annurev.pharmtox.44.101802.121453
  32. Lu, Y. and Cederbaum, A. I. : CYP2E1 and oxidative liver injury by alcohol. Free Radic. Biol. Med. 44, 723 (2008). https://doi.org/10.1016/j.freeradbiomed.2007.11.004
  33. Das, S. K. and Vasudevan, D. M. : Alcohol-induced oxidative stress. Life Sci. 81, 177 Review (2007). https://doi.org/10.1016/j.lfs.2007.05.005
  34. Liu, D., Deschamps, A., Korach, K. S. and Murphy, E. : Estrogen-enhanced gene expression of lipoprotein lipase in heart is antagonized by progesterone. Endocrinology 149, 711 (2008). https://doi.org/10.1210/en.2007-0620
  35. Morris, E. and Rymer, J. : Menopausal symptoms. Clin Evid. 10, 2138 (2003).
  36. Wronski, T. J., Dann, L. M., Qi, H. and Yen, C. F. : Skeletal effects of withdrawal of estrogen and diphosphonate treatment in ovariectomized rats. Calcif Tissue Int. 53, 210 (1993). https://doi.org/10.1007/BF01321840