Browse > Article

The Effect of Estrogen Deficiency on Ethanol Metabolism in Ovariectomized Rats  

Chang, Bo-Yoon (College of Pharmacy, Institute of Pharmaceutical Research and Development, Wonkwang University)
Kim, Sung-Yeon (College of Pharmacy, Institute of Pharmaceutical Research and Development, Wonkwang University)
Publication Information
YAKHAK HOEJI / v.54, no.6, 2010 , pp. 522-528 More about this Journal
Abstract
This study was designed to examine the effect of estrogen deficiency on the metabolism of ethanol in ovariectomized rats. Female rats were assigned to an ovariectomy (OVX) and a sham (SHAM) surgery group. Gain body weight was greater in incresed in OVX group and especially uterus weight significantly decrease depending on the concentration of estrogen after 3 month of ovariectomy. Ethanol at the tolerative dose (6 g/kg) was injected to rats by oral administration to measure the concentration of ethanol in blood. The area under the blood concentration time curve (AUC) was significantly lower in OVX group than SHAM group. The significant decrease in AUC in OVX group indicates that the estrogen deficiency leads to changes of the factors related to ethanol metabolism. Activity of hepatic alcohol dehydrogenase was not significantly influenced by the ovariectomy and also the ethanol elimination rate in vivo was not different. Cytochrome P450 isozymes did not show any changes except CYP 1A1 and 2E1. Level of hepatic glutathione in OVX group was higher after treatment of ethanol. Therefore the reduction of AUC appears not to be directly associated with the difference of ethanol metabolizing enzyme, but to be related with the physical factors like body weight.
Keywords
estrogen deficiency; ethanol; metabolism;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Das, S. K. and Vasudevan, D. M. : Alcohol-induced oxidative stress. Life Sci. 81, 177 Review (2007).   DOI   ScienceOn
2 Morris, E. and Rymer, J. : Menopausal symptoms. Clin Evid. 10, 2138 (2003).
3 Wronski, T. J., Dann, L. M., Qi, H. and Yen, C. F. : Skeletal effects of withdrawal of estrogen and diphosphonate treatment in ovariectomized rats. Calcif Tissue Int. 53, 210 (1993).   DOI   ScienceOn
4 Hamden, K., Carreau, S., Ayadi, F. and Masmoudi, H. : A Inhibitory effect of estrogens, phytoestrogens, and caloric restriction on oxidative stress and hepato-toxicity in aged rats. Biomed. Environ. Sci. 22, 381 (2009).   DOI   ScienceOn
5 Ramirez, M. E., McMurry, M. P., Wiebke, G. A., Felten, K. J., Ren, K., Meikle, A. W. and Iverius P. H. : Evidence for sex steroid inhibition of lipoprotein lipase in men: comparison of abdominal and femoral adipose tissue. Metabolism. 46, 179 (1997).   DOI   ScienceOn
6 Gandhi, M., Aweeka, F., Greenblatt, R. M. and Blaschke, T. F. : Sex differences inpharmacokinetics and pharmacodynamics. Annu. Rev. Pharmacol. Toxicol. 44, 499 (2004).   DOI   ScienceOn
7 Gloria, Rachamin J., Alain, M., Samina, W. and Jeremy, J. : Modulation of alcohol dehydrogenase and ethanol metabolism by sex hormones in the spontaneously hypertensive rat. Effect of chronic ethanol administration. Biochem. J. 15, 483 (1990).
8 Lu, Y. and Cederbaum, A. I. : CYP2E1 and oxidative liver injury by alcohol. Free Radic. Biol. Med. 44, 723 (2008).   DOI   ScienceOn
9 Liu, D., Deschamps, A., Korach, K. S. and Murphy, E. : Estrogen-enhanced gene expression of lipoprotein lipase in heart is antagonized by progesterone. Endocrinology 149, 711 (2008).   DOI
10 Zych, M., Folwarczna, J. and Trzeciak, H. I. : Natural phenolic acids may increase serum estradiol level in ovariectomized rats. Acta. Biochim. Pol. 56, 503 (2009).
11 Hidaka, I., Hino, K., Korenaga, M., Gondo, T., Nishina, S., Ando, M., Okuda, M. and Sakaida, I. : Stronger Neo- Minophagen C, a glycyrrhizin-containing preparation, protects liver against carbon tetrachloride-induced oxidative stress in transgenic mice expressing the hepatitis C virus polyprotein. Liver Int. 27, 845 (2007).   DOI   ScienceOn
12 Legros, J. J. : ADH and oxytocin in age-related minor cognitive impairment. Aging (Milano) 9, 36 (1997).
13 Harada, S., Tachiyashiki, K. and Imaizumi, K. : Effect of sex hormones on rat liver cytosolic alcohol dehydrogenase activity. J. Nutr. Sci. Vitaminol. (Tokyo) 44, 625 (1998).   DOI   ScienceOn
14 Harper C. and Kril J. : An introduction to alcohol-induced brain damage and its causes. Alcohol. Alcohol. Suppl. 2, 237 (1994).
15 Crow, K. E., Braggins, T. J., Batt, R. D. and Hardman, M. J. : Rat liver cytosolic malate dehydrogenase: purification, kinetic properties, role in control of free cytosolic NADH concentration. Analysis of control of ethanol metabolism using computer simulation. J. Biol. Chem. 257, 14217 (1982).
16 Griffith, O. W. : Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal. Biochem. 106, 207 (1980).   DOI   ScienceOn
17 Cole-Harding, S. and Wilson, J. R. : Ethanol metabolism in men and women. Journal of Studies on Alcoholism. 48, 380 (1987).   DOI
18 Ke, H. Z., Jee, W. S. S., Zeng, Q. Q., Li, M. and Lin, B. Y. : Prostaglandin E2 increased rat cortical bone mass when administered immediately following ovariectomy. Bone Miner. 21, 189 (1993).   DOI   ScienceOn
19 Allsworth, J. E., Clarke, J., Peipert, J. F., Hebert, M. R., Cooper A. and Boardman L. A. : The influence of stress on the menstrual cycle among newly incarcerated women. Womens Health Issues. 17, 202 (2007).   DOI   ScienceOn
20 Holm, A., Andersson, K. E., Nordstrom, I. and Hellstrand, P. : Nilsson BO.Down-regulation of endothelial cell estrogen receptor expression by the inflammation promoter LPS. Mol. Cell. Endocrinol. 319, 8 (2010).   DOI   ScienceOn
21 Li, X. X., Hara, I. and Matsumiya, T. : Effects of osthole on postmenopausal osteoporosis using ovariectomized rats; comparison to the effects of estradiol. Biol. Pharm. Bull. 25, 738 (2002).   DOI   ScienceOn
22 Sato, N., Lindros, K. O., Baraona, E., Ikejima, K., Mezey, E., Järveläinen, H. A. and Ramchandani, V. A. : Sex difference in alcohol-related organ injury. Alcohol. Clin. Exp. Res. 25, 40S (2001).   DOI   ScienceOn
23 Muller, C. : Liver, alcohol and gender. Wien. Med. Wochenschr. 156, 523 (2006).   DOI   ScienceOn
24 Eagon, P. K. : Alcoholic liver injury: influence of gender and hormones. World J. Gastroenterol. 16, 1377 (2010).   DOI   ScienceOn
25 Ramchandani, V. A., Bosron, W. F. and Li, T. K. : Research advances in ethanol metabolism. Pathol. Biol. 49, 676 (2001).   DOI   ScienceOn
26 Naresh, C. and Robert, H. : Analysis of alcohol 2. A review of gas chromatogaraphic method. J. Chromatogaraph Sci. 6, 263 (1972).
27 Koop, D. R., Crump, B. L., Nordblom, G. D. and Coon, M. J. : Immunochemical evidence for induction of the alcoholoxidizing cytochrome P-450 of rabbit liver microsomes by diverse agents: ethanol, imidazole, trichloroethylene, acetone, pyrazole, and isoniazid. Proc. Natl. Acad. Sci. USA 82, 4065 (1985).   DOI   ScienceOn
28 Bradford, M. M. : A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 7, 248 (1976).
29 Toran-Allerand, C. D. : Gonadal hormones and brain development: implications for the genesis of sexual differentiation. Ann. N Y Acad. Sci. 435, 101 (1984).   DOI
30 Joornvall, H., Hoooog, J. O., Bahr-Lindstroom, H., Johanson, J., Kaiser, R. and Person, R. : Alcohol dehydrogenase and aldehyde dehydrogenase. Biochem. Soc. Trans. 16, 223 (1988).   DOI
31 Mendoza-Nunez, V. M., Beristain-Perez, A., Perez-Vera, S. P. and Altamirano-Lozano, M. A. : Age-related sex differences in glutathione peroxidase and oxidative DNA damage in a healthy Mexican population. J. Womens Health (Larchmt) 19, 919 (2010).   DOI   ScienceOn
32 Ann, Macgregor, 이병석역 : Menopuase and HRT, 아카데미아, 서울 p. 26 (2005).
33 Lieber, C. S. : Alcohol and the liver: Gastroenterol. 106, 1085 (1994).   DOI
34 통계청 : 인구동채통계연보(혼인편), 통계청, 서울 (2009).
35 질병관리본부 : 국민건강영양조사(건강설문조사부분) 질병관리본부, 서울 (2009).
36 통계청 : 음주인구비율(구분별), 통계청, 서울 (1995).