Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Evaluation of Various PCR Assays for Detection of Emetic-Toxin-Producing Bacillus cereus

  • Kim, Jung-Beom (Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment) ;
  • Kim, Jae-Myung (School of Bioscience and Biotechnology, Kangwon National University) ;
  • Park, Yong-Bae (Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment) ;
  • Han, Jeong-A (Food Microbiology Division, Korea Food and Drug Administration) ;
  • Lee, Soon-Ho (Food Microbiology Division, Korea Food and Drug Administration) ;
  • Kwak, Hyo-Sun (Food Microbiology Division, Korea Food and Drug Administration) ;
  • Hwang, In-Gyun (Food Microbiology Division, Korea Food and Drug Administration) ;
  • Yoon, Mi-Hye (Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment) ;
  • Lee, Jong-Bok (Division of Health Research and Planning, Gyeonggi-do Research Institute of Health and Environment) ;
  • Oh, Deog-Hwan (School of Bioscience and Biotechnology, Kangwon National University)
  • Received : 2009.12.09
  • Accepted : 2010.02.26
  • Published : 2010.07.28
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Abstract

Because conventional methods for detecting emetic-toxin-producing B. cereus are laborious and costly, various PCR assays, which are easy and cheap, have recently been reported. Therefore, this study estimated and compared the ability of various PCR assays to detect emetic-toxin-producing B. cereus strains isolated in Korea. The PCR assays were performed on 160 B. cereus strains, including 40 emetic-toxin-producing strains. Although the species-specific PCR assays were all shown to be highly specific, the sensitivities varied greatly. The accuracies of the primers were 97.5% (CER), 95.6% (EM1), 96.3% (RE234), 89.4% (CES), and 83.1% (Ces3R/CESR2). Moreover, the CER primer had a higher sensitivity (100%) than all the other primers tested, and a specificity of 96.7%. Thus, the CER primer was shown to be the most effective for screening the emetic-toxin-producing B. cereus strains tested in this study. However, the ability of these PCR assays to identify emetic-toxin-producing B. cereus should also be confirmed using other methods.

Keywords

References

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