Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Electronic Detection of Biomarkers by Si Field-Effect Transistor from Undiluted Sample Solutions with High Ionic Strengths

  • Ah, Chil-Seong (Biosensor Research Team, Electronics and Telecommunications Research Institute) ;
  • Kim, An-Soon (Biosensor Research Team, Electronics and Telecommunications Research Institute) ;
  • Kim, Wan-Joong (Biosensor Research Team, Electronics and Telecommunications Research Institute) ;
  • Park, Chan-Woo (Biosensor Research Team, Electronics and Telecommunications Research Institute) ;
  • Ahn, Chang-Geun (Biosensor Research Team, Electronics and Telecommunications Research Institute) ;
  • Yang, Jong-Heon (Biosensor Research Team, Electronics and Telecommunications Research Institute) ;
  • Baek, In-Bok (Biosensor Research Team, Electronics and Telecommunications Research Institute) ;
  • Kim, Tae-Youb (Biosensor Research Team, Electronics and Telecommunications Research Institute) ;
  • Sung, Gun-Yong (Biosensor Research Team, Electronics and Telecommunications Research Institute)
  • Received : 2010.01.07
  • Accepted : 2010.03.29
  • Published : 2010.06.20
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Abstract

In this study, we have developed a new detection method using Si field effect transistor (FET)-type biosensors, which enables the direct monitoring of antigen-antibody binding within very high-ionic-strength solutions such as 1$\times$PBS and human serum. In the new method, as no additional dilution or desalting processes are required, the FET-type biosensors can be more suitable for ultrasensitive and real-time analysis of raw sample solutions. The new detection scheme is based on the observation that the strength of antigen-antibody-specific binding is significantly influenced by the ionic strength of the reaction solutions. For a prostate specific antigen (PSA), in some conditions, the binding reaction between PSA and anti-PSA in a low-ionic strength reaction solution such as 10 ${\mu}M$ phosphate buffer is weak (reversible), while that in high-ionic strength reaction solutions such as 1$\times$PBS or human serum is strong.

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