Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of 1,3-Dihydroxyacetone from Glycerol by Gluconobacter oxydans ZJB09112

  • Hu, Zhong-Ce (Institute of Bioengineering, Zhejiang University of Technology) ;
  • Liu, Zhi-Qiang (Institute of Bioengineering, Zhejiang University of Technology) ;
  • Zheng, Yu-Guo (Institute of Bioengineering, Zhejiang University of Technology) ;
  • Shen, Yin-Chu (Institute of Bioengineering, Zhejiang University of Technology)
  • Published : 2010.02.28
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Abstract

The culture variables were optimized to increase 1,3-dihydroxyacetone (DHA) production by Gluconohacter oxydans ZJB09112 in shake flasks and bubble column bioreactors. After fermentation in the optimized medium (g/l: yeast extract 5, glycerol 2.5, mannitol 22.5, $K_2HPO_4$ 0.5, $KH_2PO_4$ 0.5, $MgSO_4{\cdot}7H_2O$ 0.1, $CaCO_3$ 2.0, pH 5.0), when five times of glycerol feeding were applied, $161.9{\pm}5.9\;g/l$ of DHA was attained at a $88.7{\pm}3.2%$ conversion rate of glycerol to DHA.

Keywords

References

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