International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
권호 표지

Pattern of Methicillin-resistant Staphylococcus aureus in Dental and Medical Environments

  • Han, Seung-Ho (Department of Laboratory Medicine, Veterans Hospital) ;
  • Song, In-Sook (Department of Laboratory Medicine, Veterans Hospital) ;
  • Lee, Myeong-Jae (Department of Laboratory Medicine, Veterans Hospital) ;
  • Jeong, Seung-Il (Jeonju Biomaterials Institute) ;
  • Kim, Shin-Moo (Department of Clinical Laboratory Science, Wonkwang Health Science College) ;
  • Kim, Kang-Ju (Department of Oral Microbiology & Immunology, School of Dentistry, & Institute of Biotechnology Wonkwang University)
  • Received : 2010.10.28
  • Accepted : 2010.12.03
  • Published : 2010.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most prevalent pathogens in hospitals. To investigate cross contamination by this bacterium in both dental and medical settings, the pathogens that cause acute pyogenic infection and one of the major microbes responsible for nosocomial infection were isolated from health care providers, nurses and patients. We used VITEK II to measure drug sensitivity, and we further performed biochemical testing, coagulase serotype testing and pulse-field gel electrophoresis (PFGE) for isolated MRSA colonies. The isolation rate of Staphylococcus aureus from nasal swabs was 75.0% from dental health care providers and 18.8% from the medical health care providers. A total of 10 MRSA strains were isolated from 40 health care providers and 2 patients and the prevalent coagulase serotype from patients and health care providers was VII. The antimicrobial drug resistance and partial PFGE types of the isolated MRSA strains showed a similar pattern. These results suggest that MRSA may be one of the principal causes of nosocomial infection in dental and medical hospitals.

Keywords

References

  1. Aarestrup FM, Wegener HC, Rosdahl VT. Evaluation of phenotypic and genotypic methods for epidemiological typing of Staphylococcus aureus isolates from bovine mastitis in Denmark. Vet Microbiol. 1995;45:139-50. https://doi.org/10.1016/0378-1135(95)00043-A
  2. Boerlin P, Kuhnert P, Hussy D, Schaellibaum M. Methods for identification of Staphylococcus aureus isolates in cases of bovine mastitis. J Clin Microbiol. 2003;41:767-71. https://doi.org/10.1128/JCM.41.2.767-771.2003
  3. Chapin K, Musgnug M. Evaluation of three rapid methods for the direct identification of Staphylococcus aureus from positive blood cultures J Clin Microbiol. 2003;41:4324-7. https://doi.org/10.1128/JCM.41.9.4324-4327.2003
  4. Chong Y, Lee K. Present situation of antimicrobial resistance in Korea. J Infect Chemother. 2000;6:189-95 https://doi.org/10.1007/s101560070001
  5. CLSI. Performance standards for microbial susceptibility testing 19th informational supplement. M100-S19. CLSI 2009, Wayne Pa.
  6. Eriksen KR "Celbenin"-resistant staphylococci. Ugeskr Laeger. 1961;123:384-6.
  7. Etiene J, Brun Y, Fleurette J. Staphylococcus lugdunensis endocarditis. J Clin Pathol. 1989;42:892-3.
  8. Etiene J, Fleurette J, Ninet JF, Favet P, Gruer LD. Staphylococcus endocarditis after dental extraction. Lancet. 1986;2:511-2.
  9. Frency J, Brun Y, Bes M, Meugnier H, Grimont F, Grimon PAD, Newi C, Fleurette J. Staphylococcus lugdunensis sp. and Staphylococcus schleiferi sp. novel two species from human clinical specimens. Int Syst Bacteriol. 1998;38:168-72.
  10. Garner JS, Jarvis WR, Emori TG, Horan TC, Hughes JM. CDC definitions for nosocomial infections. Am J Infect Control. 1988;16:128-40. https://doi.org/10.1016/0196-6553(88)90053-3
  11. Han SH, Song IS, Kim JK, Park JG, Park JH, Lee MJ, Kim SM, Kim KJ. Monitoring of methicillin-resistant Staphylococcus aureus in Nasal Swabs Obtained from Dental Clinic Healthcare Providers and Medical Environment Nurses. Int J Oral Biol. 2010;35:7-12.
  12. Hisata K, Kuwahara-Arai K, Yamanoto M, Ito T, Nakatomi Y, Cui L, Baba T, Terasawa M, Sotozono C, Kinoshita S, Yamashiro Y, Hiramatsu K. Dissemination of methicillinresistant staphylococci among healthy Japanese children. J Clin Microbiol. 2005;43:3364-72. https://doi.org/10.1128/JCM.43.7.3364-3372.2005
  13. Holliday MG, Ford M, Perry JD, Gould FK. Rapid identification of Staphylococcus aureus by using fluorescent staphylocoagulase assays. J Clin Microbiol. 1999;37:1190-92.
  14. Hussain FM, Boyle-Vavra S, Daum RS. Community-acquired methicillin- resistant Staphylococcus aureus colonization in healthy children attending an outpatient pediatric clinic. Pediatr Infect Dis J. 2001;763-7. https://doi.org/10.1097/00006454-200108000-00009
  15. Hwang SM, Seki K, Sakurada J, Ogasawara M, Murai M, Ohmayu S, Kurosaka K, Masuda S. Improved methods for detection and serotyping of coagulase from Staphylococcus aureus. Microbiol Immunol. 1989;33:175-82. https://doi.org/10.1111/j.1348-0421.1989.tb01511.x
  16. Kim HB, Sa CM, Yoo J, Kim BS, Yun OJ, Yoon HR, Lee YS. Antibiotic resistance patterns of Staphylococcus aureus isolated from the patients admitted to non-tertiary hospitals. Korean J Infect Dis. 2000;32;259-63.
  17. Kim JM, Park ES, Jeong JS, Kim KM, Kim JM, Oh HS, Yoon SW, Chang HS, Chang KH, Lee SI, Lee MS, Song JH, Kang MW, Park SC, Choe KW, Pai CH. Multicenter surveillance study for nosocomial infections in major hospitals in Korea. Nosocomial Infection Surveillance Committee of the Korean Society for Nosocomial Infection Control. Am J Infect Control. 2000;28:454-8. https://doi.org/10.1067/mic.2000.107592
  18. Kim JS, Kim EC, Ki CS, Lee NY. Nosocomial outbreak of methicillin-resistant Staphylococcus aureus analyzed by molecular epidemiology. 1996;1:63-72
  19. Kim SM, Lee DC, Park SD, Kim BS, Kim JK, Choi MR, Park SY, Hwang SM, Shin NY, Shim ES, Kwon PS, Kwon DY, Hur SH, Kim HJ, Lim HB, Chong Y. Genotype, coagulase type and antimicrobial susceptibility of methicillin- resistant Staphylococcus aureus Isolated from dermatology patients and healthy individuals in Korea. Journal of Bacteriology and Virology. 2009;39:307-16. https://doi.org/10.4167/jbv.2009.39.4.307
  20. Kim SM, Song NK, Shin SH, Chung JO, Lee GS, Kim YH, Oh JS, Cha CD, Moon SE, Kim KJ, Shim ES, Kim EC, Seong CN, Chong Y. Nasal carrige of MRSA among healthy individual and detection of mec A and fem A gene methicillin-resistant Staphylococcus aureus. Korean J Clin Lab Sci. 1999;31:91-104.
  21. Kundsin RB. Documentation of airborne infection during surgery. Ann N Y Acad Sci. 1980;353:255-61. https://doi.org/10.1111/j.1749-6632.1980.tb18928.x
  22. Lee MS, Chong Y. Characteristics of methicillin-resistant Staphylococcus aureus isolated from wounds in Korean patients. J Infect Chemother. 1996;2:130-5. https://doi.org/10.1007/BF02351564
  23. Miller LG, Perdreau-Remington F, Rieg G, Mehdi S, Perlroth J, Bayer AS, Tang AW, Phung TO, Spellberg B. Necrotizing fasciitis caused by community- associated methicillinresistant Staphylococcus aureus in Los Angeles. N Engl J Med. 2005;352:1445-53. https://doi.org/10.1056/NEJMoa042683
  24. Moriwaki T. Genotyping of methicillin-resistant Staphylococcus aureus isolated from inpatients and medical workers in orthopaedics ward. Kansenshogaku Zasshi. 2003;77:1058-66. https://doi.org/10.11150/kansenshogakuzasshi1970.77.1058
  25. Parras F, Guerrero MC, Bouza E, Blázquez MJ, Moreno S, Menarguez MC, Cercenado E. Comparative study of mupirocin and oral co-trimoxazole plus topical fusidic acid in eradication of nasal carriage of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 1995;39:175-9. https://doi.org/10.1128/AAC.39.1.175
  26. Prevost G, Jaulhac B, Piemont Y. DNA fingerprinting by pulsefield gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates. J Clin Microbiol. 1992;30:967-73.
  27. Saravolatz LD, Markowitz N, Arking L, Pohlid D, Fisher E. Methicillin-resistant Staphylococcus aureus. Epidemiolgic observations during a community- acquired outbreak. Ann Intern Med. 1982;96:11-6. https://doi.org/10.7326/0003-4819-96-1-11
  28. Saulnier P, Bourneix C, Prévost G, Andremont A. Random amplified polymorphic DNA assay is less discriminant than pulsed-field gel electrophoresis for typing strains of methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 1993;31:982-5.
  29. Seong HK, Bae YS, Kim YH, The epidemiological of nasal colonization of methicillin-resistant Staphylococcus aureus in patients and doctors. J Exp Biomed Sci. 2004;10:309-15.
  30. Suggs AH, Maranan MC, Boyle-Vavra S, Danum RS. methicillin-resistant and borderline methicillin-resistant asymptomatic Staphylococcus aureus colonization in children without identifiable resk factors. Pediatr Infect Dis J. 1999;18:410-4. https://doi.org/10.1097/00006454-199905000-00003
  31. Tenover FC, Arbeit R, Archer G, Biddle J, Byrne S, Goering R, Hancock G, Hébert GA, Hill B, Hollis R, et al. Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus. J Clin Microbiol. 1994;32:407-15.
  32. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis criteria for bacterial strain typing. J Clin Microbiol. 1995;33:2233-9.