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바다송사리 Oryzias dancena와 자바송사리 Oryzias javanicus 간 잡종 유도

Hybridization between Marine Medaka Oryzias dancena and Javanese Medaka Oryzias javanicus

  • 송하연 (부경대학교 해양용 LMO 위해성 평가 센터) ;
  • 남윤권 (부경대학교 해양용 LMO 위해성 평가 센터) ;
  • 방인철 (순천향대학교 해양생명공학과) ;
  • 김동수 (부경대학교 해양용 LMO 위해성 평가 센터)
  • Song, Ha-Yeun (Risk Assessment Center of Marine Living Modified Organisms, Pukyong National University) ;
  • Nam, Yoon-Kwon (Risk Assessment Center of Marine Living Modified Organisms, Pukyong National University) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Kim, Dong-Soo (Risk Assessment Center of Marine Living Modified Organisms, Pukyong National University)
  • 투고 : 2010.06.28
  • 심사 : 2010.10.11
  • 발행 : 2010.10.31

초록

Inductions of hybrids and reciprocal hybrids between Oryzias dancena and O. javanicus (ODJ and OJD) were conducted and backcross hybrids between female O. dancena and male ODJ were also produced for biological and cytogenetic analysis. Embryonic development of ODJ and OJD were compared with those of their parents. Developmental time was fastest in O. dancena and ODJ, followed by O. javanicus and OJD. Oryzias dancena hatched 11 days (d) after fertilization, ODJ at 13 d, O. javanicus at 14 d and OJD at 15 d. The abnormality of external morphology rate in ODJ was 10.6%; however, OJD showed a high degree of abnormality, over 90%. The proportion of males was 90.0% and 31.3% for ODJ and OJD, respectively. Cytogenetic analysis was conducted to obtain basic information for genetic identification of O. dancena, O. javanicus and their hybrids. The karyotypes of all experimental groups showed 2n=48 chromosomes and the fundamental number (FN) was 48. The first pair carried secondary constrictions near the centromeric regions. Erythrocyte area and volume were $9.8\;{\pm}\;0.5\;{\mu}m^2$ and $18.2\;{\pm}\;1.0\;{\mu}m^3$, respectively, for O. dancena, $8.3\;{\pm}\;0.5\;{\mu}m^2$ and $15.8\;{\pm}\;1.5\;{\mu}m^3$ in O. javanicus, and $18.3\;{\pm}\;0.5\;{\mu}m^2$ and $15.7\;{\pm}\;1.3\;{\mu}m^3$ in ODJ. Erythrocyte area and volume in ODJ were similar to those of O. javanicus. In backcross hybrids between female O. dancena and male ODJ, all embryos failed to develop and died in the late gastrula stage.

키워드

참고문헌

  1. Ari K. 1988. Viability of allotriploids in salmonids. Bull Jap Soc Sci Fish 54, 1695-1701. https://doi.org/10.2331/suisan.54.1695
  2. Benfey TJ. 1989. A bibliography of triploid fish, 1943 to 1988. Can Tech Rep Fish Aquat Sci 1682, 33.
  3. Cho YS, Lee SY, Kim DS and Nam YK. 2010. Tolerance capacity to salinity changes in adult and larva of Oryzias dancena, a euryhaline medaka. Kor J Ichthyol 22, 9-16.
  4. Derenzini M. 2000. The AgNORs. Micron 31, 117-120. https://doi.org/10.1016/S0968-4328(99)00067-0
  5. Glamuzina B, Glavi N, Skaramuca B, Koul V and Tutman P. 2001. Early development of the hybrid Epinephelus costae ${\times}$ E. marginatus . Aquaculture 198, 55-61. https://doi.org/10.1016/S0044-8486(00)00511-1
  6. Glamuzina B, Skaramuca B, Glavi N and Koul V. 1998. Egg and larval development of the dusky grouper, Epinephelus marginatus (Lowe, 1834). Sci Mar 62, 373-378.
  7. Gupta SD, Khan HA, Reddy PVGK and Kowtal GV. 1986. Studies on the intergenetic hybrid, common carp, Cyprinus carpio communis L. x mrigal, cirrhinus mrigala (Hamilton). Establishment of a Network of Aquaculture Centres in Asia project reports, FAO Fisheries & Aquaculture, 1-10.
  8. Hori R and Iwamatsu T. 1996. Experiments on interspecific hybridization among Oryzias melastigma, Oryzias javanicus and Oryzias latipes. Bull Oraki Women's College 37, 1-6.
  9. Inoue K and Takei Y. 2003. Asian medaka fishes offer new models for studying mechanisms of seawater adaptation. Comp Biochem Physiol B 136, 635-645. https://doi.org/10.1016/S1096-4959(03)00204-5
  10. Iwamatsu T, Kobayashi H, Yamashita M, Shibata Y and Yusa A. 2003. Experiments hybridization among Oryzias species. II. Karyogamy and abnormality of chromosome separation in the cleavage of interspecific hybrids between Oryzias latipes and O. javanicus. Zool Sci 20, 1381-1387. https://doi.org/10.2108/zsj.20.1381
  11. Iwamatsu T, Mori T and Hori R. 1994. Experimental hybridization among Oryzias species I. O. celebensis, O. javanicus, O. latipes, O. luzoenesis and O. melastigma. Bull Aichi Univ of Edu 43, 103-112.
  12. Iwamatsu T, Watanabe T, Hori R, Lam TJ and Saxena OP. 1986. Experiments on interspecific hybridization between Oryzias melastigma and Oryzias javanicus. Zool Sci 3, 287-293.
  13. Iwamatsu T and Hirata K. 1984. Normal course of development of the Java medaka, Oryzias javanicus. Bull Aichi Univ Edu 33, 87-109.
  14. Iwamatsu T, Uwa H, Inden A and Hirata K. 1984. Experiments on interspecific hybridization between Oryzias latipes and Oryzias celebensis. Zool Sci 1, 653-663.
  15. Kakuno A, Fujii K and Koyama J. 2001. Histological studies on the gonadal development in Java medaka (Oryzias javanicus). Bull Fish Res Agen 1, 35-37.
  16. Kim DS, Nam YK and Park IS. 1995a. Performance of diploid and triploid hybrid loach, Misgurnus mizolepis female ${\times}$ M. anguillicaudatus male. Aquaculture 137, 326-327.
  17. Kim KK, Bang IC, Kim Y and Kim DS. 1995b. Survival and growth in larval stage of induced hybrid between olive flounder (Paralichthys olivaceus) and spotted flounder (Verasper variegatus). J Aquaculture 8, 69-76.
  18. Kim IS and Kim SY. 1993. Interspecific Hybridization between Oryzias sinensis and O. latipes from Korea. Kor J Ichthyol 5, 113-121.
  19. Kim DS, Park EH and Kim JS. 1982. Karyotypes of nine species of the Korean catfihses (Teleostomi: Siluriformes). Kor J Genet 4, 57-68.
  20. Lemoine HL Jr and Smith LT. 1980. Polyploidy induced in brook trout by cold shock. Trans Fish Soc 109, 626-631. https://doi.org/10.1577/1548-8659(1980)109<626:PIIBTB>2.0.CO;2
  21. Li J, Zhang Q, Qi J, Wang X and Wang Z. 2006. Embryonic and larval development in hybrid between Paralichthys olivaceus (female) and Verasper variegates (male). J Fish Sci China 13, 732-739.
  22. Nam YK. 1998. Cytogenetic analysis of induced hybrid between common carp (Cyprinus carpio) and crucian carp (Carassius auratus). J Aquaculture 1, 77-81.
  23. Naruse K. 1996. Classification and phylogeny of fishes of the genus Oryzias and its relatives. Fish Biol J Medaka 8, 1-9.
  24. Nelson JS. 2006. Fishes of the World. 4th edition, Wiley & Sons Inc, New York, U.S.A. 276-277.
  25. Palti Y, Nochols KM, Waller KI, Parsons JE and Thorgaard GH. 2001. Association between DNA polymorphisms tightly linked to MHC class II genes and IHN virus resistance in backcrosses of rainbow and cutthroat trout. Aquaculture 194, 283-289. https://doi.org/10.1016/S0044-8486(00)00526-3
  26. Park IS, Kim BS, Hur JW, Iraida GS, Kim DS, Im JH and Park IS. 2004. Cytogenitic analysis of an artificial Red () Black seabream () Hybid. Kor J Genet 26, 283-288.
  27. Park IS, Nam YK, Douglas SE, Johnson SC and Kim DS. 2003. Genetic characterization, morphometrics and gonad development of induced interspecific hybrids between yellowtail flounder, Pleuronectes ferruginesu (Storer) and winter flounder, Pleuronectes americanus (Walbaum). Aquacult Res 34, 389-396. https://doi.org/10.1046/j.1365-2109.2003.00816.x
  28. Park IS, Kim BS, Im JH, Park HM, Nam YK, Jeong CH and Kim DS. 1997. Improved early survival in backcrosses of male mud loach (Misgurnus mizolepis) ${\times}$ cyprinid loach (M. anguillicaudatus) hybrid to female cyprinid loach. J Aquaculture 10, 363-371.
  29. Park IS, Kim PK, Kim JM, Choi GC and Kim DS. 1996. Production of hybrid and allotriploid between rainbow trout (Oncorhynchus mykiss) and coho Salmon (O. kisutch). J Aquaculture 9, 133-140.
  30. Phillips RB and Ihssen PE. 1985. Chromosome banding in salmonid fishes; nucleolar organizer regions in Salmo and Salvelinus. Can J Genet Cytol 27, 433-440. https://doi.org/10.1139/g85-064
  31. Robert TR. 1998. Systematic observations on tropical Asian medakas or ricefishes of the genus Oryzias, with descriptions of four new species. Ichthyol Res 45, 213-224. https://doi.org/10.1007/BF02673919
  32. Sakai C, Konno F, Nakani O, Iwai T, Yokota T, Lee J, Nishida-Umehara C, Kuroiwa A, Matsuda Y and Yamashita M. 2007. Chromosome elimination in the interspecific hybrid medaka between Oryzias latipes and O. hubbsi. Chromosome Research 15, 697-709. https://doi.org/10.1007/s10577-007-1155-9
  33. Sezaki K and Kobayashi H. 1978. Comparison of erythrocytic size between diploid and tetraploid in spinous loach, Cobitis biwae. Bull Jap Soc Sci Fish 41, 851-854.
  34. Shinomiya A, Kato M, Yaezawa M, Sakaizumi M and Hamaguchi S. 2006. Interspecific hybridization between Oryzias latipes and Oryzias curvinotus causes XY sex reversal. J Exp Zoolo 305, 890-896.
  35. Song HY, Bang IC, Nam YK and Kim DS. 2009a. Early gonadogenesis and sex differentiation of a marine medaka, Oryzias dancena (Beloniformes; Teleostei). Kor J Ichthyol 21, 141-148.
  36. Song HY, Bang IC, Nam YK and Kim DS. 2009b. Embryogenesis and early ontogenesis of a marine medaka, Oryzias dancena. Kor J Ichthyol 21, 227-238.
  37. Susuki N and Hibiya T. 1984. Development of eggs larvae of two bitterlings, Rhodeus atremius and R. suigensis (Cyprinidae). Jap J Ichthyol 31, 287-296.
  38. Takehana Y, Hamaguchi S and Sakaizumi M. 2008. Different origins of ZZ/ZW sex chromosomes in closely related medaka fishes, Oryzias javanicus and O. hubbsi. Chromosome Research 16, 801-811. https://doi.org/10.1007/s10577-008-1227-5
  39. Takehana Y, Naruse K, Hamaguchi S and Sakaizumi M. 2007a. Evolution of ZZ/ZW and XX/XY sex-determination systems in the closely related medaka species, Oryzias hubbsi and O. dancena. Chromosoma 116, 463-470. https://doi.org/10.1007/s00412-007-0110-z
  40. Takehana Y, Demiyah D, Naruse K, Hamaguchi S and Sakaizumi M. 2007b. Evolution of different Y chromosomes in two medaka species, Oryzias dancena and O. latipes. Genetics 175, 1335-1340.
  41. Takehana Y, Naruse K and Sakaizumi M. 2005. Molecular phylogeny oh the medaka fishes genus Oryzias (Beloniformes: Adrianichthyidae) based on nuclear and mitochondrial DNA sequences. Mol Phylohenet Evol 36, 417-428. https://doi.org/10.1016/j.ympev.2005.01.016
  42. Takeuchi K. 1960. A study of the mutant (wavy) in medaka, Oryzias latipes. Annot Zool Japon 33, 124-131.
  43. Uwa H, Uyeno T, Arai R, Taniuchi T and Sakaizumi M. 1986. Karyotype evolution and geographical distribution in the ricefish, genus Oryzias (Oryziidae) Indo-Pacific fish biology: proceedings of the second international conference on Indo-Pacific fishers. Ichthyological Society of Japan, Tokyo, 867-876.
  44. Uwa H and Iwata A. 1981. Karyotype and cellular DNA content of Oryzias javanicus (Oryziatidae, Pisces). Chromosome Inf Serv 31, 24-26.

피인용 문헌

  1. Genomic Organization, Intronic Duplications, and Promoter Characteristics of the Fast Skeletal Myosin Light Chain-2 Gene (mlc2f) from Javanese Ricefish Oryzias javanicus vol.15, pp.4, 2012, https://doi.org/10.5657/FAS.2012.0325
  2. Molecular Characterization of Cytoskeletal Beta-Actin and its Promoter in the Javanese Ricefish Oryzias javanicus vol.15, pp.4, 2012, https://doi.org/10.5657/FAS.2012.0317
  3. Cytogenetic analysis of hybrids and hybrid triploids between the river puffer, Takifugu obscurus , and the tiger puffer, Takifugu rubripes vol.49, pp.2, 2018, https://doi.org/10.1111/are.13493
  4. Isolation of Two Hepcidin Paralogs, Hamp1 and Hamp2, from a Euryhaline Javanese Ricefish (Oryzias javanicus: Beloniformes) vol.14, pp.2, 2011, https://doi.org/10.5657/FAS.2011.0093
  5. 바다송사리, Oryzias dancena와 자바송사리, O. javanicus 간 잡종에 대한 비스페놀 A의 효과 vol.24, pp.2, 2010, https://doi.org/10.7847/jfp.2011.24.2.131