Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Hybridization between Marine Medaka Oryzias dancena and Javanese Medaka Oryzias javanicus

바다송사리 Oryzias dancena와 자바송사리 Oryzias javanicus 간 잡종 유도

  • Song, Ha-Yeun (Risk Assessment Center of Marine Living Modified Organisms, Pukyong National University) ;
  • Nam, Yoon-Kwon (Risk Assessment Center of Marine Living Modified Organisms, Pukyong National University) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Kim, Dong-Soo (Risk Assessment Center of Marine Living Modified Organisms, Pukyong National University)
  • 송하연 (부경대학교 해양용 LMO 위해성 평가 센터) ;
  • 남윤권 (부경대학교 해양용 LMO 위해성 평가 센터) ;
  • 방인철 (순천향대학교 해양생명공학과) ;
  • 김동수 (부경대학교 해양용 LMO 위해성 평가 센터)
  • Received : 2010.06.28
  • Accepted : 2010.10.11
  • Published : 2010.10.31
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Abstract

Inductions of hybrids and reciprocal hybrids between Oryzias dancena and O. javanicus (ODJ and OJD) were conducted and backcross hybrids between female O. dancena and male ODJ were also produced for biological and cytogenetic analysis. Embryonic development of ODJ and OJD were compared with those of their parents. Developmental time was fastest in O. dancena and ODJ, followed by O. javanicus and OJD. Oryzias dancena hatched 11 days (d) after fertilization, ODJ at 13 d, O. javanicus at 14 d and OJD at 15 d. The abnormality of external morphology rate in ODJ was 10.6%; however, OJD showed a high degree of abnormality, over 90%. The proportion of males was 90.0% and 31.3% for ODJ and OJD, respectively. Cytogenetic analysis was conducted to obtain basic information for genetic identification of O. dancena, O. javanicus and their hybrids. The karyotypes of all experimental groups showed 2n=48 chromosomes and the fundamental number (FN) was 48. The first pair carried secondary constrictions near the centromeric regions. Erythrocyte area and volume were $9.8\;{\pm}\;0.5\;{\mu}m^2$ and $18.2\;{\pm}\;1.0\;{\mu}m^3$, respectively, for O. dancena, $8.3\;{\pm}\;0.5\;{\mu}m^2$ and $15.8\;{\pm}\;1.5\;{\mu}m^3$ in O. javanicus, and $18.3\;{\pm}\;0.5\;{\mu}m^2$ and $15.7\;{\pm}\;1.3\;{\mu}m^3$ in ODJ. Erythrocyte area and volume in ODJ were similar to those of O. javanicus. In backcross hybrids between female O. dancena and male ODJ, all embryos failed to develop and died in the late gastrula stage.

Keywords

References

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