한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제43권5호
  • /
  • Pages.437-444
  • /
  • 2010
  • /
  • 0374-8111(pISSN)
  • /
  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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해양 미세조류 Nannochloropsis oculata 추출.분획물의 ACE, α-glucosidase 및 암세포 저해 활성

ACE, α-Glucosidase and Cancer Cell Growth Inhibitory Activities of Extracts and Fractions from Marine Microalgae, Nannochloropsis oculata

  • 차선희 (한국기초과학지원연구원 해양바이오연구팀) ;
  • 김민주 (한국기초과학지원연구원 해양바이오연구팀) ;
  • 양혜영 (한국기초과학지원연구원 해양바이오연구팀) ;
  • 진창범 (한국기초과학지원연구원 해양바이오연구팀) ;
  • 전유진 (제주대학교 해양의생명과학부) ;
  • ;
  • 김대경 (한국기초과학지원연구원 해양바이오연구팀)
  • Cha, Seon-Heui (Marine Bio Research Team, Korea Basic Science Institute (KBSI)) ;
  • Kim, Min-Joo (Marine Bio Research Team, Korea Basic Science Institute (KBSI)) ;
  • Yang, Hye-Young (Marine Bio Research Team, Korea Basic Science Institute (KBSI)) ;
  • Jin, Chang-Beum (Marine Bio Research Team, Korea Basic Science Institute (KBSI)) ;
  • Jeon, You-Jin (School of Marine Biomedical Sciences, Jeju National University) ;
  • Oda, Tatsuya (Division of Biochemistry, Faculty of fisheries, Nagasaki University) ;
  • Kim, Dae-Kyung (Marine Bio Research Team, Korea Basic Science Institute (KBSI))
  • 투고 : 2010.09.08
  • 심사 : 2010.10.11
  • 발행 : 2010.10.31
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초록

Extracts of the marine microalgae Nannochloropsis oculata were obtained using 80% methanol (MeOH) and water. The 80% MeOH extract was further fractionated with n-hexane, chloroform, ethyl acetate (EtOAc), n-butanol (n-BuOH), and water to isolate the active fraction. Seven samples were prepared and their angiotensin converting enzyme (ACE), $\alpha$-glucosidase, and cancer cell growth inhibitory activities in vitro were determined. The most profound ACE inhibitory activity was observed in the chloroform fraction, while the others had moderate effects. By contrast, greater $\alpha$-glucosidase inhibitory activity was found in the EtOAc fraction, n-hexane fraction, and water extract of N. oculata. The antiproliferative effects of the extracts and fractions against HL-60, U937, CT-26, and SK-Hep1 cancer cells were also determined. The n-BuOH fraction had the strongest antiproliferative effects on CT-26 cells in a time-dependant manner (P<0.05). These results suggest that the extracts and fractions from N. oculata could be used as a potential functional food or as pharmaceutical ingredients.

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피인용 문헌

  1. Development of Economical Fertilizer-Based Media for Mass Culturing of Nannochloropsis oceanica vol.14, pp.4, 2011, https://doi.org/10.5657/FAS.2011.0317