Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of In-vitro Anticoagulation Activity of 35 Different Seaweed Extracts

35종 해조류 추출물의 in-vitro 항혈전 활성 평가

  • Ahn, Seon-Mi (Department of Food and Nutrition, Andong National University) ;
  • Hong, Yong-Ki (Department of Biotechnology, Pukyong National University) ;
  • Kwon, Gi-Seok (The School of Bioresource Science, Andong National University) ;
  • Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
  • 안선미 (안동대학교 식품영양학과) ;
  • 홍용기 (부경대학교 생물공학과) ;
  • 권기석 (안동대학교 생명자원과학부) ;
  • 손호용 (안동대학교 식품영양학과)
  • Received : 2010.08.11
  • Accepted : 2010.10.01
  • Published : 2010.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Seaweeds have been recognized as a health food, having anti-obesity, anti-constipation and anticoagulation activities, and the use of seaweeds in the food, medicine, and cosmetic industries have recently significantly increased. In this study, methanol extracts were prepared from 35 different seaweeds (17 phaeophyta, 11 rhodophyta and 7 chlorophyta), and thrombin time (TT), prothrombin time (PT) and activated partial thromboplastin time (aPTT) were determined in order to develop safe and novel anticoagulation agents from natural products. In TT experiments, Ecklonia cava, Ecklonia stolonifera, Eisenia bicyclis (Kjellman) Setchell, Ishige foliacea, I. okamurai, Sargassum confusum and S. yamade showed strong thrombin inhibition activity among the 35 different seaweeds. In PT experiments, the inhibitions of prothrombin were identified in the selected seaweeds from TT experiment, with the exception of S. yamade. In aPTT experiments, the seaweeds with blood coagulation inhibition factors were E. cava, E. stolonifera, E. bicyclis (Kjellman) Setchell, I. foliacea, I. okamurai, S. confusum and Hixikia fusiforme Okamura. Further anticoagulation assay with the selected 8 seaweeds suggested that S. confusum is most effective in antithrombosis, and E. stolonifera, E. bicyclis (Kjellman) Setchell, and I. foliacea have high potential as antithrombosis agents. Based on components-activity correlation analysis, flavonoids are considered as active anticoagulation components of seaweeds These results suggest that edible seaweeds, especially S. confusum, have potential as safe and novel anticoagulants, and S. yamade and H. fusiforme Okamura could be used as a thrombin-specific and coagulation factor-specific inhibitors.

혈전성 질환을 예방, 개선할 수 있는 안전한 항혈전제를 개발하기 위해, 일반적으로 항혈전 효과가 있다고 알려진 식용 해조류 35종(갈조류 17종, 홍조류 11종 및 녹조류 7종)을 대상으로 메탄올 추출물을 조제하고, 이들의 thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (aPTT)을 평가하였다. 트롬빈 저해(TT)의 경우 35종 중에서 감태, 곰피, 대황, 넓패, 패, 알송이모자반 및 야마다모자반에서 강력한 활성을, 프로트롬빈 저해(PT)의 경우 트롬빈 저해활성이 나타난 7종 중 야마다모자반을 제외한 6종에서 강력한 활성을 확인하였으며, 혈액응고인자 저해(aPTT)에서는 감태, 곰피, 대황, 넓패, 패, 알송이모자반 및 톳에서 강력한 활성이 나타났다. 선별된 8종의 시료를 대상으로 다양한 농도에서 항혈전 활성을 평가한 결과 알송이모자반에서 가장 강력한 활성을 확인하였으며, 곰피, 대황 및 넓패가 효과적이었다. 항혈전 활성과 해조류 추출물의 총 flavonoid, 총 polyphenol, 총당 및 환원당 함량과의 상관 관계를 검토한 결과, 활성물질은 flavonoid성 물질로 추측되었으며, 선별된 8종은 총 flavonoid 함량 및 총 polyphenol 함량이 매우 높은 특징을 나타내었다. 본 연구 결과는 경제적이면서 대량공급이 가능한 식용 해조류로부터 신규의 안전한 항혈전제 개발이 가능함을 제시하며, 야마다모자반과 톳은 트롬빈 또는 혈장내 응고 인자에 대한 특이 저해제로 개발 가능함을 제시하고 있다.

Keywords

References

  1. Butenas, S. and S. Mullertz. 2002. Blood coagulation. Biochemistry (Moscow) 67, 3-12. https://doi.org/10.1023/A:1013985911759
  2. Choi, I. W., S. U. Kim, D. C. Seo, B. H. Kang, B. K. Sohn, Y. S. Rim, J. S. Heo, and J. S. Cho. 2005. Biosorption of heavy metals biomass of seaweeds, Laminaria species, Ecklonia stolonifera, Gelidium amansii, and Undaria pinnatifida. Korean J. Environ. Agri. 24, 370-378. https://doi.org/10.5338/KJEA.2005.24.4.370
  3. Eikelboom, J. W., S. L. Zelenkofske, and C. P. Rusconi. 2010. Coagulation factor IXa as a target for treatment and prophylaxis of venous thromboembolism. Arterioscler. Thromb. Vasc. Biol. 30, 382-387. https://doi.org/10.1161/ATVBAHA.110.203117
  4. Hsieh, K. H. 1997. Thrombin interaction with fibrin polymerization sites. Thromb. Res. 86, 301-316. https://doi.org/10.1016/S0049-3848(97)00073-X
  5. Jin, H. J. and D. H. Jin. 2007. Screening of seaweed extracts for algicidal substances using a photosensitization effect. J. Korean Fish Soc. 40, 122-127. https://doi.org/10.5657/kfas.2007.40.3.122
  6. Joo, D. S., J. K. Lee, Y. S. Choi, S. Y. Cho, Y. K. Je, and J. W. Choi. 2003. Effect of sea tangle oligosaccharide drink on serum and hepatic lipids in rats fed a hyperlipidemic diet. J. Korean Soc. Food Sci. Nutr. 32, 1364-1369. https://doi.org/10.3746/jkfn.2003.32.8.1364
  7. Kang, M. C., J. Y. Lee, R. K. Ko, H. B. Kim, S. H. Hong, and G. O. Kim. 2008. Melanin inhibitory effect and anti-inflammatory effects of Dietyota coriacea extracts derived from adjacent sea of the Jeju island. Korean J. Biotechnol. Bioeng. 23, 311-316.
  8. Kang, S. Y., M. J. Oh, and J. A. Shin. 2005. Antimicrobial activity of korean marine algae against fish pathogenic bacteria. J. Fish Pathol. 18, 147-156.
  9. Kim, D. W., M. J. Kim, T. S. Shin, S. J. Kim, and B. M. Jung. 2008. Application of hydrogen peroxide on the bacterial control of seaweed Capsosiphon fulvescens (Mesaengi). Korean J. Food Preserv. 15, 169-173.
  10. Kim, J. A. and J. M. Lee. 2004. The changes of biologically functional compounds and antioxidant activities in Hizikia fusiformis with drying methods. Korean J. Food Culture 19, 200-208.
  11. Kim, J. H., D. S. Lee, C. W. Lim, H. Y. Park, and J. H. Park. 2002. Antibacterial activity of sea-mustard, Laminaria japonica extracts on the cariogenic bacteria, Streptococcus mutans. J. Korean Fish Soc. 35, 191-195. https://doi.org/10.5657/kfas.2002.35.2.191
  12. Kim, M. D., S. K. Rhee, and J. H. Seo. 2001. Enhanced production of anticoagulant hirudin in recombinant Saccharomyces cerevisiae by chromosomal $\delta$-integration. J. Biotechnol. 85, 41-48. https://doi.org/10.1016/S0168-1656(00)00376-X
  13. Kim, S. A., J. Kim, M. K. Woo, C. S. Kwak, and M. S. Lee. Antimutagenic and cytotoxic effects of ethanol extracts from five kinds of seaweeds. J. Korean Soc. Food Sci. Nutr. 34, 451-459. https://doi.org/10.3746/jkfn.2005.34.4.451
  14. Kong, C. S., Y. R. Um, J. I. Lee, Y. A. Kim, J. S. Lee, and Y. W. Seo. 2008. Inhibition effects of extracts and its solvent fractions isolated from Limonium tetragonum on growth of human cancer cells. J. Biotechnol. Bioeng. 23, 177-182.
  15. Koo, J. G., Y. S. Choi, and J. K. Kwak. 2001. Blood-anticoagulation activity of fucoidans from sporophylls of Undaria pinnatifida, Laminaria religiosa, Hizikia fusiforme, and Sargassum fulvellum in Korea. J. Korean Fish Soc. 34, 515-520.
  16. Kwon, C. S., Y. S. Kwon, Y. S. Kim, G. S. Kwon, I. N. Jin, G. C. Ryu, and H. Y. Sohn. 2004. Inhibitory activities of edible and medicinal herbs against human thrombin. J. Life Sci. 14, 509-513. https://doi.org/10.5352/JLS.2004.14.3.509
  17. Lee, H. O., D. S. Kim, J. R. Do, and Y. S. Ko. 1999. Angiotensin-I converting enzyme inhibitory activity of algae. J. Korean Fish Soc. 32, 427-431.
  18. Lee, H. S., J. H. Suh, and K. H. Suh. 2000. Preparation of antibacterial agent from seaweed extract and its antibacterial effect. J. Korean Fish. Soc. 33, 32-37.
  19. Lim, C. W. 2000. Structure and some properties of the antimicrobial compounds in the red alga, Symphylocladia latiuscula. J. Korean Fish Soc. 33, 280-287.
  20. Lim, J. H., K. S. Jung, J. S. Lee, E. S. Jung, D. K. Kim, Y. S. Kim, Y. W. Kim, and D. H. Park. 2008. The study on antimicrobial and antifungal activity of the wild seaweeds of Jeju island. J. Soc. Cosmet Sci. Korean 34, 201-207.
  21. Nikapitiya, C., M. Zoysa, P. M. Ekanyake, H. J. Park, and J. H. Lee. 2006. Isolation and purification of anticoagulant polysaccharide compound from fermented edible brown seaweed, Laminaria ochotensis. J. Aquaculture 19, 33-39.
  22. Oh, J. K., Y. O. Shin, H. S. Sohn, and R. M. Seo. 2003. Effect of functional food including seaweeds extracts supplementation on hematological variables and antioxidant system. Korean J. Physical Education 42, 895-903.
  23. Ryu, H. Y., E. J. Kum, K. H. Bae, Y. K. Kim, I. S. Kwun, and H. Y. Sohn. 2007. Evaluation for the antimicrobial, antioxidant and anti-thrombosis activity of Korean traditional liquors. Korean J. Microbiol. Biotechnol. 35, 238-244.
  24. Ryu, H. Y., J. C. Heo, J. S. Hwang, S. W. Kang, C. Y. Yun, S. H. Lee, and H. Y. Sohn. 2008. Screening of thrombin inhibitor and its DPPH radical scavenging activity from wild insects. J. Life Sci. 18, 363-368. https://doi.org/10.5352/JLS.2008.18.3.363
  25. Ryu, H. Y., K. H. Bae, E. J. Kum, S. J. Park, B. H. Lee, and H. Y. Sohn. 2007. Evaluation of antimicrobial, antioxidant and antithrombosis activity of natural spices for fresh-cut yam. J. Life Sci. 17, 652-657. https://doi.org/10.5352/JLS.2007.17.5.652
  26. Ryu, H. Y., S. M. Ahn, J. S. Kim, and H. Y. Sohn. 2010. Evaluation of in-vitro anticoagulation activity of 33 different medicinal herbs. J. Life Sci. 20, 922-928. https://doi.org/10.5352/JLS.2010.20.6.922
  27. Sohn, H. Y., C. S. Kwon, K. H. Son, G. S. Kwon, W. S. Kwon, H. Y. Ryu, and E. J. Kum. 2005. Antithrombosis and antioxidant activity of methanol extract from different brands of rice. J. Korean Soc. Food Sci. Nutr. 34, 593-598. https://doi.org/10.3746/jkfn.2005.34.5.593
  28. Sohn, H. Y., H. Y. Ryu, Y. S. Kwon, E. J. Kum, C. S. Kwon, G. S. K won, K. W. Kim, and K. H. Son. 2005. Screening of thrombin inhibitor from medicinal and wild plants (II). Korean J. Pharmacogn. 36, 263-272.
  29. Sohn, H. Y., Y. S. Kwon, Y. S. Kim, H. Y. Kwon, G. S. Kwon, G. J. Kim, C. S. Kwon, and K. H. Son. 2004. Screening of thrombin inhibitors from medicinal and wild plants. Korean J. Pharmacogn. 35, 52-61.
  30. Yoon, J. A., K. W. Yu, W. J. Jun, H. Y. Cho, Y. S. Son, and H. C. Yang. 2000. Screening of anticoagulant activity in the extracts of edible seaweeds and optimization of extraction condition. J. Korean Soc. Food Sci. Nutr. 29, 1098-1106.

Cited by

  1. Laminaria japonica increases plasma exposure of glycyrrhetinic acid following oral administration of Liquorice extract in rats vol.13, pp.7, 2015, https://doi.org/10.1016/S1875-5364(15)30049-2
  2. Beneficial effect of seaweed on high-fat diet-induced oxidative stress and insulin resistance in rats vol.24, pp.6, 2015, https://doi.org/10.1007/s10068-015-0291-9
  3. Antioxidant Effect and Tyrosinase Inhibition Activity of Seaweeds Ethanol Extracts vol.42, pp.12, 2013, https://doi.org/10.3746/jkfn.2013.42.12.1893
  4. Antioxidant and α-glucosidase inhibition activity of seaweed extracts vol.22, pp.2, 2015, https://doi.org/10.11002/kjfp.2015.22.2.290
  5. Effect of Sea Tangle (<italic>Laminaria japonica</italic>) and Charcoal Supplementation as Alternatives to Antibiotics on Growth Performance and Meat Quality of Ducks vol.27, pp.2, 2014, https://doi.org/10.5713/ajas.2013.13314
  6. Evaluation of Antioxidant, Anti-Inflammatory, Antithrombotic, and Antiobesity Activities in Cultured Edible Plants to Increase Farm Income vol.28, pp.1, 2017, https://doi.org/10.7856/kjcls.2017.28.1.29
  7. In vitro antioxidant potential and inhibitory effect of seaweed on enzymes relevant for hyperglycemia vol.23, pp.6, 2014, https://doi.org/10.1007/s10068-014-0277-z
  8. Characteristics of marine algae extracts using subcritical water extract method vol.21, pp.1, 2014, https://doi.org/10.11002/kjfp.2014.21.1.62
  9. Evaluation of Antioxidant and Nitrite Scavenging Activity of Seaweed Extracts vol.21, pp.4, 2011, https://doi.org/10.5352/JLS.2011.21.4.576
  10. Anti-thrombosis and anti-oxidative activity of the root of Arctium lappa L. vol.21, pp.5, 2014, https://doi.org/10.11002/kjfp.2014.21.5.727