Reproductive and Developmental Biology

  • 제34권3호
  • /
  • Pages.175-180
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  • 2010
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Efficient Labeling of Porcine Hematopoietic Cells by Fluorescence-Conjugated Nanoparticles

  • Lee, Hyun-Joo (Dept. of Biomedical Science, College of Life Science, CHA University) ;
  • Park, Eun-Ji (Dept. of Biomedical Science, College of Life Science, CHA University) ;
  • Lee, Yong-Soo (Dept. of Biomedical Science, College of Life Science, CHA University) ;
  • Park, Sung-Won (Dept. of Biomedical Science, College of Life Science, CHA University) ;
  • Kim, Jae-Hwan (Dept. of Biomedical Science, College of Life Science, CHA University) ;
  • Kim, Dong-Ku (Dept. of Biomedical Science, College of Life Science, CHA University)
  • 투고 : 2010.08.26
  • 심사 : 2010.09.17
  • 발행 : 2010.09.30
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초록

Nanotechnology is currently receiving considerable attention in various fields of biotechnology. The uptake of nanoparticles by cells for labeling and tracking is a critical process for many biomedical therapeutic applications. However, nanoparticle labeling of porcine hematopoietic cells has not been demonstrated so far. In the present study, silica-coated nanoparticles conjugated with rhodamine B isothiocyanate (SR-RITC) were used to investigate the uptake of nanoparticles by porcine hematopoietic cells. Flow cytometric and confocal microscopic analyses reveled that the cells were efficiently internalized by the silica-coated nanoparticles. Furthermore, biocompatibility tests demonstrated that the SR nanoparticles were not cytotoxic, and they had no impact on proliferation. Our study demonstrates that silica-coated nanoparticles are taken up very rapidly and with high efficiency into porcine hematopoietic cells, with no apparent deleterious effects. Therefore, silica-coated nanoparticles appear to be a promising tool for tracking porcine hematopoietic cells.

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참고문헌

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